RT Journal Article SR Electronic(1) A1 Hatrongjit, Rujirat A1 Kerdsin, Anusak A1 Gottschalk, Marcelo A1 Hamada, Shigeyuki A1 Oishi, Kazunori A1 Akeda, YukihiroYR 2016 T1 Development of a multiplex PCR assay to detect the major clonal complexes of Streptococcus suis relevant to human infection JF Journal of Medical Microbiology, VO 65 IS 5 SP 392 OP 396 DO https://doi.org/10.1099/jmm.0.000239 PB Microbiology Society, SN 1473-5644, AB Multilocus sequence typing (MLST) is considered a reliable method for providing insight into the Streptococcus suis population structure, clonal relationships and the potential of particular clones to cause disease. Indeed, MLST has revealed the presence of several clonal complexes (CCs) within the Streptococcus suis population. However, the method is costly, time-consuming and difficult to use for screening large numbers of isolates. In this study, a multiplex PCR assay was developed to identify Streptococcus suis CCs that are relevant to human infections. The multiplex PCR assay was capable of simultaneously distinguishing CC1, CC25, CC28, CC104, CC221/234 and CC233/379, which are related to human infections in Thailand, in a single reaction. The multiplex PCR assay is useful for low-cost screening of large numbers of isolates with rapid analytical capacity and could be utilized in most laboratories., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000239