@article{mbs:/content/journal/jmm/10.1099/jmm.0.000256, author = "Singh, Namita and Prasad, Priya and Singh, Laishram Chandreshwar and Das, Banashree and Rastogi, Sangita", title = "Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters", journal= "Journal of Medical Microbiology", year = "2016", volume = "65", number = "6", pages = "476-483", doi = "https://doi.org/10.1099/jmm.0.000256", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000256", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "progesterone", keywords = "recurrent spontaneous abortion", keywords = "gestational age", keywords = "prostaglandin receptors", keywords = "Chlamydia trachomatis", keywords = "endometrial curettage tissue", abstract = "A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched controls (Group II) at the Department of Obstetrics and Gynecology, Safdarjung Hospital, New Delhi (India). PCR was performed for diagnosis of C. trachomatis cryptic plasmid; mRNA expression of PG receptor genes was assessed by real-time PCR (q-PCR), while serum progesterone/estrogen levels were determined by respective commercial kits. Data were evaluated statistically. A total of 15.4 % RSA (GroupI) were diagnosed as C. trachomatis-positive (200 bp), whereas controls were uninfected. q-PCR showed significant upregulation (P<0.0001) of PGE2 (EP-1, EP-2, EP-3, EP-4), PGF2α (FP) and PGI2 (IP) receptors in Group I versus Group II. The expression of PG receptors increased significantly with advanced gestational age (P<0.002); however, only contractile receptors, EP-1, EP-3 and FP, were positively correlated with gestational age in Group-I. In infected RSA, mean serum progesterone level was significantly low (P<0.0001) while serum oestrogen was high (P<0.0001). Overall, the data suggest that increased expression of PG receptors, particularly contractile gene receptors (EP-1, EP-3, FP), with advanced gestational age and altered steroid levels could be a possible risk factor for abortion in Chlamydia-infected RSA.", }