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Volume 65, Issue 7

Rapid and combined detection of , Epstein–Barr virus and human cytomegalovirus using AllGlo quadruplex quantitative PCR Free

Abstract

Acute respiratory infections (ARIs) cause substantial morbidity and mortality worldwide. The causes of ARI are dynamic, and co-infections of , Epstein–Barr virus and human cytomegalovirus are recently developed causes of ARI. Here, we established a quadruplex quantitative PCR (qPCR) method to rapidly identify and simultaneously detect a single infection or co-infection of these three pathogens and an internal control in a single tube using AllGlo probes. The analysis demonstrated a wide linear range of detection from 10to 10 copies per test and a low coefficient of variation of less than 5 %. The amplification efficiencies were all close to 1, and the correlation coefficients ( ) were all greater than 0.99. We found no significant difference in a comparative reagent test ( >0.05). Moreover, the results of tests on clinical samples using AllGlo quadruplex qPCR and TaqMan uniplex qPCR were in near-perfect agreement ( =0.97). Clinically, the availability of this method will enable better differential diagnosis, disease surveillance and controlled outcomes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): AllGlo probes , Epstein–Barr virus , internal control , Mycoplasma pneumoniae and quadruplex quantitative PCR
© 2016 The Authors
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/content/journal/jmm/10.1099/jmm.0.000266
2016-07-01
2024-04-20
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Rapid and combined detection of Mycoplasma pneumoniae, Epstein–Barr virus and human cytomegalovirus using AllGlo quadruplex quantitative PCR
J. Med. Microbiol. 65, 590 (2016); https://doi.org/10.1099/jmm.0.000266
/content/journal/jmm/10.1099/jmm.0.000266
/content/journal/jmm/10.1099/jmm.0.000266
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