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f Enterohepatic Helicobacter spp. in cats with non-haematopoietic intestinal carcinoma: a survey of 55 cases

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  • Authors: Alton G. Swennes1​ ,† ,  Nicola M. A. Parry1​ ,  Yan Feng1​ ,  Erin Sawyer1​ ,‡ ,  Bryan R. Lohr2​ ,  David C. Twedt3​ , James G. Fox1​
    • VIEW AFFILIATIONS
    • 1​ 1​ Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA 2​ 2​ Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA 3​ 3​ Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA
  • Correspondence Alton G. Swennes [email protected]
  • First Published Online: 01 August 2016, Journal of Medical Microbiology 65: 814-820, doi: 10.1099/jmm.0.000274
  • Subject: Pathogenicity and Virulence/Host Response
  • Received:
  • Accepted:
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  • Several enterohepatic Helicobacter spp. (EHS) have been isolated from cats. Despite the reported association between EHS infection and intestinal neoplasia in other species, this association has not been explored in cats. In this study, 55 non-haematopoietic feline intestinal carcinoma cases were histopathologically evaluated. In contrast with prior reports, large intestinal (LI) carcinoma was observed with greater frequency (61 %) relative to small intestinal (SI) carcinoma (35 %). There was a significant association between intestinal location and animal gender. Of males examined, 83 % had LI carcinoma, while no such trend was observed in females. Previously described associations between Siamese breed and intestinal carcinoma could not be definitively confirmed, although the Siamese breed may be predisposed to SI carcinoma location. Of all carcinomas examined in this study, 62 % were classified as adenocarcinoma, although mucinous adenocarcinoma (28 %) and solid carcinoma (11 %) were also identified. Tumours were all moderately or poorly differentiated. When considered by intestinal location and histopathologic classification, LI adenocarcinoma was associated with significantly advanced mean age (13 years) when compared to SI adenocarcinoma and LI mucinous adenocarcinoma (mean, 9 years in both cases), which were also frequently encountered. To determine whether EHS might play a role in feline intestinal neoplasia, Helicobacter genus- and species-specific fluorescence in situ hybridization was performed. Of these carcinoma cases, 56 % were positive for Helicobacter spp. and one or more species-specific assay for Helicobacter bilis, Helicobacter canis or Helicobacter marmotae. The presence of EHS was significantly associated with both LI location (68 %) and mucinous adenocarcinoma (92 %). These findings suggest a role for intestinal bacteria in non-haematopoietic feline intestinal neoplasia.

  • †

    Center for Comparative Medicine and Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA

  • ‡

    Laboratory Animal Science Center, Boston University Medical Campus, Boston, MA, USA

  • One supplementry table is available with the online Supplementary Material.

  • Abbreviations:
    EHS enterohepatic Helicobacter spp.
    FISH fluorescence in situ hybridization
    ICC ileocecocolic
    LI large intestinal
    SI small intestinal

© 2016 The Authors | Published by the Microbiology Society

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