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Journal of Medical Microbiology logo

Volume 65, Issue 9

after 50 years: is it a neglected pathogen? Free

Abstract

It has been 50 years since the first case of primary amoebic meningoencephalitis (PAM), an acute and rapidly fatal disease of the central nervous system (CNS), was reported in Australia. It is now known that the aetiological agent of PAM is , an amoeba that is commonly known as ‘the brain-eating amoeba’. infects humans of different ages who are in contact with water contaminated with this micro-organism. is distributed worldwide and is found growing in bodies of freshwater in tropical and subtropical environments. The number of PAM cases has recently increased, and the rate of recovery from PAM has been estimated at only 5 %. Amphotericin B has been used to treat patients with PAM. However, it is important to note that there is no specific treatment for PAM. Moreover, this amoeba is considered a neglected micro-organism. Researchers have exerted great effort to design effective drugs to treat PAM and to understand the pathogenesis of PAM over the past 50 years, such as its pathology, molecular and cellular biology, diagnosis and prevention, and its biological implications, including its pathogenic genotypes, its distribution and its ecology. Given the rapid progression of PAM and its high mortality rate, it is important that investigations continue and that researchers collaborate to gain better understanding of the pathogenesis of this disease and, consequently, to improve the diagnosis and treatment of this devastating infection of the CNS.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Diagnosis , Naegleria fowleri , Pathogenicity , Primary amoebic meningoencephalitis and Treatment
© 2016 The Authors
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2016-09-01
2024-04-19
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References

  1. Adl S. M., Simpson A. G., Farmer M. A., Andersen R. A., Anderson O. R., Barta J. R., Bowser S. S., Brugerolle G., Fensome R. A. et al. 2005; The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J Eukaryot Microbiol 52:399–451 [View Article][PubMed]
     [Google Scholar]
  2. Aldape K., Huizinga H., Bouvier J., McKerrow J. 1994; Naegleria fowleri: characterization of a secreted histolytic cysteine protease. Exp Parasitol 78:230–241 [View Article][PubMed]
     [Google Scholar]
  3. Alonso P., Zubiaur E. 1985; Phagocytic activity of three Naegleria strains in the presence of erythrocytes of various types. J Protozool 32:661–664[PubMed] [CrossRef]
     [Google Scholar]
  4. Anderson K., Jamieson A. 1972; Agglutination test for the investigation of the genus Naegleria . Pathology 4:273–278 [View Article][PubMed]
     [Google Scholar]
  5. Apley J., Clarke S. K., Roome A. P., Sandry S. A., Saygi G., Silk B., Warhurst D. C. 1970; Primary amoebic meningoencephalitis in Britain. Br Med J 1:596–599 [View Article][PubMed]
     [Google Scholar]
  6. Baig A. M., Khan N. A. 2015; Tackling infection owing to brain-eating amoeba. Acta Trop 142:86–88 [View Article][PubMed]
     [Google Scholar]
  7. Behets J., Seghi F., Declerck P., Verelst L., Duvivier L., Van Damme A., Ollevier F. 2003; Detection of Naegleria spp. and Naegleria fowleri: a comparison of flagellation tests, ELISA and PCR. Water Sci Technol 47:117–122[PubMed]
     [Google Scholar]
  8. Belofsky G., Carreno R., Goswick S. M., John D. T. 2006; Activity of isoflavans of Dalea aurea (Fabaceae) against the opportunistic amoeba Naegleria fowleri . Planta Med 72:383–386 [View Article][PubMed]
     [Google Scholar]
  9. Brajtburg J., Bolard J. 1996; Carrier effects on biological activity of amphotericin B. Clin Microbiol Rev 9:512–531[PubMed]
     [Google Scholar]
  10. Brown R. L. 1991; Successful treatment of primary amebic meningoencephalitis. Arch Intern Med 151:1201–1202 [View Article][PubMed]
     [Google Scholar]
  11. Budge P. J., Lazensky B., Van Zile K. W., Elliott K. E., Dooyema C. A., Visvesvara G. S., Beach M. J., Yoder J. S. 2013; Primary amebic meningoencephalitis in Florida: a case report and epidemiological review of Florida cases. J Environ Health 75:26–31[PubMed]
     [Google Scholar]
  12. Butt C. G., Baro C., Knorr R. W. 1968; Naegleria (sp.) identified in amebic encephalitis. Am J Clin Pathol 50:568–574 [View Article][PubMed]
     [Google Scholar]
  13. Centers for Disease Control and Prevention (CDC) 2013; Investigational drug available directly from CDC for the treatment of infections with free-living amoebae. MMWR Morb Mortal Wkly Rep 62:666[PubMed]
     [Google Scholar]
  14. Carrasco-Yepez M., Campos-Rodriguez R., Godinez-Victoria M., Rodriguez-Monroy M. A., Jarillo-Luna A., Bonilla-Lemus P., De Oca A. C., Rojas-Hernandez S. 2013; Naegleria fowleri glycoconjugates with residues of alpha-D-mannose are involved in adherence of trophozoites to mouse nasal mucosa. Parasitol Res 112:3615–3625 [View Article][PubMed]
     [Google Scholar]
  15. Carter R. F. 1969; Sensitivity to amphotericin B of a Naegleria sp. isolated from a case of primary amoebic meningoencephalitis. J Clin Pathol 22:470–474 [View Article][PubMed]
     [Google Scholar]
  16. Carter R. F. 1970; Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis, and of the experimental pathological changes induced by it. J Pathol 100:217–244 [View Article][PubMed]
     [Google Scholar]
  17. Carter R. F., Cullity G. J., Ojeda V. J., Silberstein P., Willaert E. 1981; A fatal case of meningoencephalitis due to a free-living amoeba of uncertain identity – probably Acanthamoeba sp. Pathology 13:51–68 [View Article][PubMed]
     [Google Scholar]
  18. Cavalier-Smith T., Fiore-Donno A. M., Chao E., Kudryavtsev A., Berney C., Snell E. A., Lewis R. 2015; Multigene phylogeny resolves deep branching of Amoebozoa. Mol Phylogenet Evol 83:293–304 [View Article][PubMed]
     [Google Scholar]
  19. Cerva L. 1969; Amoebic meningoencephalitis: axenic culture of Naegleria . Science 163:576[PubMed]
     [Google Scholar]
  20. Cerva L., Novak K. 1968; Amoebic meningoencephalitis: 16 fatalities. Science 160:92 [CrossRef]
     [Google Scholar]
  21. Cerva L., Novak K., Culbertson C. G. 1968; An outbreak of acute, fatal amebic meningoencephalitis. Am J Epidemiol 88:436–444
     [Google Scholar]
  22. Cervantes-Sandoval I., Serrano-Luna J., Garcia-Latorre E., Tsutsumi V., Shibayama M. 2008a; Characterization of brain inflammation during primary amoebic meningoencephalitis. Parasitol Int 57:307–313 [CrossRef]
     [Google Scholar]
  23. Cervantes-Sandoval I., Serrano-Luna J., Garcia-Latorre E., Tsutsumi V., Shibayama M. 2008b; Mucins in the host defence against Naegleria fowleri and mucinolytic activity as a possible means of evasion. Microbiology 154:3895–3904 [CrossRef]
     [Google Scholar]
  24. Cervantes-Sandoval I., Serrano-Luna J., Pacheco-Yépez J., Silva-Olivares A., Tsutsumi V., Shibayama M. 2010; Differences between Naegleria fowleri and Naegleria gruberi in expression of mannose and fucose glycoconjugates. Parasitol Res 106:695–701 [View Article][PubMed]
     [Google Scholar]
  25. Chang S. L. 1979; Pathogenesis of pathogenic Naegleria amoeba. Folia Parasitol 26:195–200[PubMed]
     [Google Scholar]
  26. Chávez-Munguía B., Omaña-Molina M., Castañón G., Bonilla P., González-Lázaro M., Hernández-Martínez D., Salazar-Villatoro L., Esparza-García A., Martínez-Palomo A., Ortega-Pierres G. 2009; Ultrastructural study of the encystation and excystation processes in Naegleria sp. J Eukaryot Microbiol 56:66–72 [View Article][PubMed]
     [Google Scholar]
  27. Chávez-Munguía B., Segovia-Gamboa N., Salazar-Villatoro L., Omaña-Molina M., Espinosa-Cantellano M., Martínez-Palomo A. 2011; Naegleria fowleri: enolase is expressed during cyst differentiation. J Eukaryot Microbiol 58:463–468 [View Article][PubMed]
     [Google Scholar]
  28. Cogo P. E., Scagli M., Gatti S., Rossetti F., Alaggio R., Laverda A. M., Zhou L., Xiao L., Visvesvara G. S. 2004; Fatal Naegleria fowleri meningoencephalitis, Italy. Emerg Infect Dis 10:1835–1837 [View Article][PubMed]
     [Google Scholar]
  29. Cope J. R., Conrad D. A., Cohen N., Cotilla M., DaSilva A., Jackson J., Visvesvara G. S. 2016; Use of the novel therapeutic agent miltefosine for the treatment of primary amoebic meningoencephalitis: report of 1 fatal and 1 surviving case. Clin Infect Dis 62:774–776 [View Article][PubMed]
     [Google Scholar]
  30. Cubero-Menéndez O., Cubero-Rego D. 2004; Primary amoebic meningoencephalitis: a case report. Rev Neurol 38:336–338[PubMed]
     [Google Scholar]
  31. da Rocha-Azevedo B., Tanowitz H. B., Marciano-Cabral F. 2009; Diagnosis of infections caused by pathogenic free-living amoebae. Interdis Perspect Infect Dis 2009:1–14 [View Article]
     [Google Scholar]
  32. De Jonckheere J. F. 1982; Isoenzyme patterns of pathogenic and non-pathogenic Naegleria spp. using agarose isoelectricfocusing. Ann Microbiol 133:319–342
     [Google Scholar]
  33. De Jonckheere J. F. 2004; Molecular definition and the ubiquity of species in the genus Naegleria . Protist 155:89–103 [View Article][PubMed]
     [Google Scholar]
  34. De Jonckheere J. F. 2011; Origin and evolution of the worldwide distributed pathogenic amoeboflagellate Naegleria fowleri . Infect Genet Evol 11:1520–1528 [View Article][PubMed]
     [Google Scholar]
  35. De Jonckheere J. F. 2012; The impact of man on the occurrence of the pathogenic free-living amoeboflagellate Naegleria fowleri . Future Microbiol 7:5–7 [View Article][PubMed]
     [Google Scholar]
  36. De Jonckheere J. F. 2014; What do we know by now about the genus Naegleria?. Exp Parasitol 145:1–8 [View Article][PubMed]
     [Google Scholar]
  37. Debnath A., Tunac J. B., Galindo-Gomez S., Silva-Olivares A., Shibayama M., McKerrow J. H. 2012; Corifungin, a new drug lead against Naegleria, identified from a high-throughput screen. Antimicrobial Agen Chemother 56:5450–5457 [View Article]
     [Google Scholar]
  38. Diaz J. H., Boudreaux J. P. 2013; Emerging trends in free-living amebic infections of the brain: implications for organ transplantation. J La State Med Soc 165:314–318[PubMed]
     [Google Scholar]
  39. Dorlo T. P., Balasegaram M., Beijnen J. H., De Vries P. J. 2012; Miltefosine: a review of its pharmacology and therapeutic efficacy in the treatment of leishmaniasis. J Antimicrob Chemother 67:2576–2597 [View Article][PubMed]
     [Google Scholar]
  40. Duma R. J., Rosenblum W. I., McGehee R. F., Jones M. M., Nelson E. C. 1971; Primary amoebic meningoencephalitis caused by Naegleria. Two new cases, response to amphotericin B, and a review. Ann Intern Med 74:923–931[PubMed] [CrossRef]
     [Google Scholar]
  41. Dyková I., Kyselová I., Pecková H., Oborník M., Lukes J. 2001; Identity of Naegleria strains isolated from organs of freshwater fishes. Dis Aquat Organ 46:115–121 [View Article][PubMed]
     [Google Scholar]
  42. Feldman M. R. 1977; Naegleria fowleri: fine structural localization of acid phosphatase and heme proteins. Exp Parasitol 41:283–289 [View Article][PubMed]
     [Google Scholar]
  43. Ferrante A., Thong Y. H. 1979; Antibody induced capping and endocytosis of surface antigens in Naegleria fowleri . Int J Parasitol 9:599–601 [View Article][PubMed]
     [Google Scholar]
  44. Fowler M., Carter R. F. 1965; Acute pyogenic meningitis probably due to Acanthamoeba sp.: a preliminary report. Br Med J 2:740–742 [View Article][PubMed]
     [Google Scholar]
  45. Fritz-Laylin L. K., Cande W. Z. 2010; Ancestral centriole and flagella proteins identified by analysis of Naegleria differentiation. J Cell Sci 123:4024–4031 [View Article]
     [Google Scholar]
  46. Fritz-Laylin L. K., Prochnik S. E., Ginger M. L., Dacks J. B., Carpenter M. L., Field M. C., Kuo A., Paredez A., Chapman J. et al. 2010; The genome of Naegleria gruberi illuminates early eukaryotic versatility. Cell 140:631–642 [View Article]
     [Google Scholar]
  47. Fritz-Laylin L. K., Ginger M. L., Walsh C., Dawson S. C., Fulton C. 2011; The Naegleria genome: a free-living microbial eukaryote lends unique insights into core eukaryotic cell biology. Res Microbiol 162:607–618 [View Article][PubMed]
     [Google Scholar]
  48. Fulton C. 1977; Intracellular regulation of cell shape and motility in Naegleria. First insights and a working hypothesis. J Supramol Struct 6:13–43 [View Article][PubMed]
     [Google Scholar]
  49. Han K. L., Lee H. J., Shin M. H., Shin H. J., Im K. I., Park S. J. 2004; The involvement of an integrin-like protein and protein kinase C in amoebic adhesion to fibronectin and amoebic cytotoxicity. Parasitol Res 94:53–60 [View Article][PubMed]
     [Google Scholar]
  50. Hannisch W., Hallagan L. F. 1997; Primary amebic meningoencephalitis: a review of the clinical literature. Wild Environ Med 8:211–213 [View Article]
     [Google Scholar]
  51. Hara T., Fukuma T. 2005; Diagnosis of the primary amoebic meningoencephalitis due to Naegleria fowleri . Parasitol Int 54:219–221 [View Article]
     [Google Scholar]
  52. Heggie T. W. 2010; Swimming with death: Naegleria fowleri infections in recreational waters. Travel Med Infect Dis 8:201–206 [View Article][PubMed]
     [Google Scholar]
  53. Herbst R., Ott C., Jacobs T., Marti T., Marciano-Cabral F., Leippe M. 2002; Pore-forming polypeptides of the pathogenic protozoon Naegleria fowleri . J Biol Chem 277:22353–22360 [View Article][PubMed]
     [Google Scholar]
  54. Hysmith R. M., Franson R. C. 1982; Elevated levels of cellular and extracellular phospholipases from pathogenic Naegleria fowleri . Biochim Biophys Acta 711:26–32 [View Article][PubMed]
     [Google Scholar]
  55. Jaffar-Bandjee M. C., Alessandri J. L., Molet B., Clouzeau J., Jacquemot L., Sampériz S., Saly J. C. 2005; Primary amebic meningoencephalitis: 1st case observed in Madagascar. Bull Soc Pathol Exot 98:11–13[PubMed]
     [Google Scholar]
  56. Jain R., Prabhakar S., Modi M., Bhatia R., Sehgal R. 2002; Naegleria meningitis: a rare survival. Neurol India 50:470–472[PubMed]
     [Google Scholar]
  57. Jamerson M., Da Rocha-Azevedo B., Cabral G. A., Marciano-Cabral F. 2012; Pathogenic Naegleria fowleri and non-pathogenic Naegleria lovaniensis exhibit differential adhesion to, and invasion of, extracellular matrix proteins. Microbiology 158:791–803 [View Article][PubMed]
     [Google Scholar]
  58. Jarolim K. L., McCosh J. K., Howard M. J., John D. T. 2000; A light microscopy study of the migration of Naegleria fowleri from the nasal submucosa to the central nervous system during the early stage of primary amebic meningoencephalitis in mice. J Parasitol 86:50–55 [View Article][PubMed]
     [Google Scholar]
  59. John D. T. 1982; Primary amebic meningoencephalitis and the biology of Naegleria fowleri . Ann Rev Microbiol 36:101–123 [View Article]
     [Google Scholar]
  60. Kaur H., Seifert K., Hawkes G. E., Coumbarides G. S., Alvar J., Croft S. L. 2015; Chemical and bioassay techniques to authenticate quality of the anti-leishmanial drug miltefosine. Am J Trop Med Hyg 92:31–38 [View Article][PubMed]
     [Google Scholar]
  61. Kidney D. D., Kim S. H. 1998; CNS infections with free-living amebas: neuroimaging findings. AJR Am J Roentgenol 171:809–812 [View Article][PubMed]
     [Google Scholar]
  62. Kim J. H., Jung S. Y., Lee Y. J., Song K. J., Kwon D., Kim K., Park S., Im K. I., Shin H. J. 2008a; Effect of therapeutic chemical agents in vitro and on experimental meningoencephalitis due to Naegleria fowleri . Antimicrob Agen Chemother 52:4010–4016 [View Article]
     [Google Scholar]
  63. Kim J. H., Kim D., Shin H. J. 2008b; Contact-independent cell death of human microglial cells due to pathogenic Naegleria fowleri trophozoites. Korean J Parasitol 46:217–221 [View Article]
     [Google Scholar]
  64. Kim J. H., Lee Y. J., Sohn H. J., Song K. J., Kwon D., Kwon M. H., Im K. I., Shin H. J. 2008c; Therapeutic effect of rokitamycin in vitro and on experimental meningoencephalitis due to Naegleria fowleri . Int J Antimicrob Agen 32:411–417 [View Article]
     [Google Scholar]
  65. Klemba M., Goldberg D. E. 2002; Biological roles of proteases in parasitic protozoa. Annu Rev Biochem 71:275–305 [View Article][PubMed]
     [Google Scholar]
  66. Kyle D. E., Noblet G. P. 1985; Vertical distribution of potentially pathogenic free-living amoebae in freshwater lakes. J Protozool 32:99–105[PubMed] [CrossRef]
     [Google Scholar]
  67. Kyle D. E., Noblet G. P. 1987; Seasonal distribution of thermotolerant free-living amoebae. II. Lake Issaqueena. J Protozool 34:10–15 [View Article][PubMed]
     [Google Scholar]
  68. Lares-Villa F., De Jonckheere J. F., De Moura H., Rechi-Iruretagoyena A., Ferreira-Guerrero E., Fernandez-Quintanilla G., Ruiz-Matus C., Visvesvara G. S. 1993; Five cases of primary amebic meningoencephalitis in Mexicali, Mexico: study of the isolates. J Clin Microbiol 31:685–688[PubMed]
     [Google Scholar]
  69. Lares-Villa F. 2001; Free-living amoebae infections in Mexico. In Proceedings IXth International Meeting on the Biology and Pathogenicity of Free-Living Amoebae Paris, France: John Libbey Eurotext;
     [Google Scholar]
  70. Lares-Villa F., Hernández-Peña C. 2010; Concentration of Naegleria fowleri in natural waters used for recreational purposes in Sonora, Mexico (November 2007–October 2008). Exp Parasitol 126:33–36 [View Article][PubMed]
     [Google Scholar]
  71. Lee J., Kim J. H., Sohn H. J., Yang H. J., Na B. K., Chwae Y. J., Park S., Kim K., Shin H. J. 2014; Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory-secretory proteins and their biochemical properties. Parasitol Res 113:2765–2776 [View Article][PubMed]
     [Google Scholar]
  72. Lee Y. J., Kim J. H., Jeong S. R., Song K. J., Kim K., Park S., Park M. S., Shin H. J. 2007; Production of Nfa1-specific monoclonal antibodies that influences the in vitro cytotoxicity of Naegleria fowleri trophozoites on microglial cells. Parasitol Res 101:1191–1196 [View Article][PubMed]
     [Google Scholar]
  73. Levine N. D., Corliss J. O., Cox F. E., Deroux G., Grain J., Honigberg B. M., Leedale G. F., Loeblich A. R., Lom J. et al. 1980; A newly revised classification of the protozoa. J Protozool 27:37–58 [View Article][PubMed]
     [Google Scholar]
  74. Linam W. M., Ahmed M., Cope J. R., Chu C., Visvesvara G. S., Da Silva A. J., Qvarnstrom Y., Green J. 2015; Successful treatment of an adolescent with Naegleria fowleri primary amebic meningoencephalitis. Pediatrics 135:e744748 [View Article][PubMed]
     [Google Scholar]
  75. Loschiavo F., Ventura-Spagnolo T., Sessa E., Bramanti P. 1993; Acute primary meningoencephalitis from Naegleria fowleri: report of a clinical case with a favourable outcome. Acta Neurol 15:333–340
     [Google Scholar]
  76. López-Corella E., De León B., De Jonckheere J. F. 1989; Primary amebic meningoencephalitis caused by Naegleria fowleri in an adolescent from Huetamo, Michoacan, Mexico. Bol Med Hosp Infant Mex 46:619–622[PubMed]
     [Google Scholar]
  77. Maclean R. C., Richardson D. J., LePardo R., Marciano-Cabral F. 2004; The identification of Naegleria fowleri from water and soil samples by nested PCR. Parasitol Res 93:211–217 [View Article][PubMed]
     [Google Scholar]
  78. Madarová L., Trnková K., Feiková S., Klement C., Obernauerová M. 2010; A real-time PCR diagnostic method for detection of Naegleria fowleri . Exp Parasitol 126:37–41 [View Article][PubMed]
     [Google Scholar]
  79. Marciano-Cabral F., John D. T. 1983; Cytopathogenicity of Naegleria fowleri for rat neuroblastoma cell cultures: scanning electron microscopy study. Infect Immun 40:1214–1217[PubMed]
     [Google Scholar]
  80. Marciano-Cabral F. 1988; Biology of Naegleria spp. Microbiol Rev 52:114–133[PubMed]
     [Google Scholar]
  81. Marciano-Cabral F., Cabral G. A. 2007; The immune response to Naegleria fowleri amebae and pathogenesis of infection. FEMS Immunol Med Microbiol 51:243–259 [View Article][PubMed]
     [Google Scholar]
  82. Martinez A. J., Nelson E. C., Jones M. M., Duma R. J., Rosenblum W. I. 1971; Experimental Naegleria meningoencephalitis in mice. An electron microscope study. Lab Invest 25:465–475[PubMed]
     [Google Scholar]
  83. Martinez A. J., Visvesvara G. S. 1991; Laboratory diagnosis of pathogenic free-living amoebas: Naegleria, Acanthamoeba, and Leptomyxid . Clin Lab Med 11:861–872[PubMed]
     [Google Scholar]
  84. Martinez A. J., Visvesvara G. S. 1997; Free-living, amphizoic and opportunistic amebas. Brain Pathol 7:583–598 [View Article][PubMed]
     [Google Scholar]
  85. Martinez J., Duma R. J., Nelson E. C., Moretta F. L. 1973; Experimental naegleria meningoencephalitis in mice. Penetration of the olfactory mucosal epithelium by Naegleria and pathologic changes produced: a light and electron microscope study. Lab Invest 29:121–133[PubMed]
     [Google Scholar]
  86. Martínez-Castillo M., Ramírez-Rico G., Serrano-Luna J., Shibayama M. 2015; Iron-binding protein degradation by cysteine proteases of Naegleria fowleri . Biomed Res Int 2015:416712–416718 [View Article][PubMed]
     [Google Scholar]
  87. Mat Amin N. 2004; Proteinases in Naegleria fowleri (strain NF3), a pathogenic amoeba: a preliminary study. Trop Biomed 21:57–60[PubMed]
     [Google Scholar]
  88. McKerrow J. H., Rosenthal P. J., Swenerton R., Doyle P. 2008; Development of protease inhibitors for protozoan infections. Curr Opin Infect Dis 21:668–672 [View Article][PubMed]
     [Google Scholar]
  89. Mishra B. B., Gundra U. M., Teale J. M. 2009; Toll-like receptors in CNS parasitic infections. Curr Top Microbiol Immunol 336:83–104 [View Article][PubMed]
     [Google Scholar]
  90. Naqi R., Azeemuddin M. 2013; Naeglaeria infection of the central nervous system, CT scan findings: a case series. J Pak Med Assoc 63:399–402[PubMed]
     [Google Scholar]
  91. Norton R., Harris P., Ryan P., Simpson S. 2010; Another killer of the Australian bush: a rapidly fatal meningoencephalitis in a child. Clin Infect Dis 50:1422–1424 [CrossRef]
     [Google Scholar]
  92. Pantchev N., Tappe D. 2011; Pentastomiasis and other parasitic zoonoses from reptiles and amphibians. Berl Munch Tierarztl Wochenschr 124:528–535[PubMed]
     [Google Scholar]
  93. Patterson M., Woodworth T. W., Marciano-Cabral F., Bradley S. G. 1981; Ultrastructure of Naegleria fowleri enflagellation. J Bacteriol 147:217–226[PubMed]
     [Google Scholar]
  94. Poungvarin N., Jariya P. 1991; The fifth nonlethal case of primary amoebic meningoencephalitis. J Med Assoc Thai 74:112–115[PubMed]
     [Google Scholar]
  95. Pélandakis M., Serre S., Pernin P. 2000; Analysis of the 5.8S rRNA gene and the internal transcribed spacers in Naegleria spp. and in N. fowleri . J Eukaryot Microbiol 47:116–121 [View Article][PubMed]
     [Google Scholar]
  96. Qvarnstrom Y., Da Silva A. J., Schuster F. L., Gelman B. B., Visvesvara G. S. 2009; Molecular confirmation of Sappinia pedata as a causative agent of amoebic encephalitis. J Infect Dis 199:1139–1142 [View Article][PubMed]
     [Google Scholar]
  97. Rice C. A., Colon B. L., Alp M., Goker H., Boykin D. W., Kyle D. E. 2015; Bis-Benzimidazole hits against Naegleria fowleri discovered with new high-throughput screens. Antimicrob Agen Chemother 59:2037–2044 [View Article]
     [Google Scholar]
  98. Rivera-Aguilar V., Hernández-Martínez D., Rojas-Hernández S., Oliver-Aguillón G., Tsutsumi V., Herrera-González N., Campos-Rodríguez R. 2000; Immunoblot analysis of IgA antibodies to Naegleria fowleri in human saliva and serum. Parasitol Res 86:775–780 [View Article][PubMed]
     [Google Scholar]
  99. Robinson B. S., Monis P. T., Dobson P. J. 2006; Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis. Appl Environ Microbiol 72:5857–5863 [View Article][PubMed]
     [Google Scholar]
  100. Rodríguez-Zaragoza S. 1994; Ecology of free-living amoebae. Crit Rev Microbiol 20:225–241 [View Article][PubMed]
     [Google Scholar]
  101. Rojas-Hernández S., Jarillo-Luna A., Rodríguez-Monroy M., Moreno-Fierros L., Campos-Rodríguez R. 2004; Immunohistochemical characterization of the initial stages of Naegleria fowleri meningoencephalitis in mice. Parasitol Res 94:31–36 [View Article][PubMed]
     [Google Scholar]
  102. Réveiller F. L., Cabanes P. A., Marciano-Cabral F. 2002; Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri . Parasitol Res 88:443–450 [View Article][PubMed]
     [Google Scholar]
  103. Sajid M., McKerrow J. H. 2002; Cysteine proteases of parasitic organisms. Mol Biochem Parasitol 120:1–21 [View Article][PubMed]
     [Google Scholar]
  104. Scaglia M., Gatti S., Brustia R., Chichino G., Rondanelli E. G. 1991; Phagocytosis of human erythrocytes by Naegleria is not related to species pathogenicity. A phase-contrast cinemicrographic study. Microbiologica 14:45–53[PubMed]
     [Google Scholar]
  105. Schuster F. 1963; An electron microscope study of the amoebo-flagellate, Naegleria gruberi (Schardinger). I. The amoeboid and flagellate stages. J Protozool 10:297–313 [CrossRef]
     [Google Scholar]
  106. Schuster F. L., Rechthand E. 1975; In vitro effects of amphotericin B on growth and ultrastructure of the amoeboflagellates Naegleria gruberi and Naegleria fowleri . Antimicrob Agents Chemother 8:591–605 [View Article][PubMed]
     [Google Scholar]
  107. Schuster F. L., Visvesvara G. S. 2004; Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol 34:1001–1027 [View Article][PubMed]
     [Google Scholar]
  108. Serrano-Luna J., Cervantes-Sandoval I., Tsutsumi V., Shibayama M. 2007; A biochemical comparison of proteases from pathogenic Naegleria fowleri and non-pathogenic Naegleria gruberi . J Eukaryot Microbiol 54:411–417 [View Article][PubMed]
     [Google Scholar]
  109. Shakoor S., Beg M. A., Mahmood S. F., Bandea R., Sriram R., Noman F., Ali F., Visvesvara G. S., Zafar A. 2011; Primary amebic meningoencephalitis caused by Naegleria fowleri, Karachi, Pakistan. Emerg Infect Dis 17:258–261 [View Article][PubMed]
     [Google Scholar]
  110. Sharma A., Guleria S. 2015; Successful treatment of a case of primary amoebic meningoencephalitis: how important is history taking. Indian J Crit Care Med 19:126–127 [View Article][PubMed]
     [Google Scholar]
  111. Shibayama M., Serrano-Luna J., Rojas-Hernández S., Campos-Rodríguez R., Tsutsumi V. 2003; Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I. Can J Microbiol 49:164–170 [View Article][PubMed]
     [Google Scholar]
  112. Shibayama M., Martínez-Castillo M., Silva-Olivares A., Galindo-Gómez S., Navarro-García F., Escobar-Herrera J., Sabanero M., Tsutsumi V., Serrano-Luna J. 2013; Disruption of MDCK cell tight junctions by the free-living amoeba Naegleria fowleri . Microbiology 159:392–401 [View Article][PubMed]
     [Google Scholar]
  113. Siddiqui R., Khan N. A. 2014; Primary amoebic meningoencephalitis caused by Naegleria fowleri: an old enemy presenting new challenges. PLoS Negl Trop Dis 8:e3017 [View Article][PubMed]
     [Google Scholar]
  114. Sifuentes L. Y., Choate B. L., Gerba C. P., Bright K. R. 2014; The occurrence of Naegleria fowleri in recreational waters in Arizona. J Environ Sci Health, Part A 49:1322–1330 [View Article]
     [Google Scholar]
  115. Simpson A. G. 2003; Cytoskeletal organization, phylogenetic affinities and systematics in the contentious taxon Excavata (Eukaryota). Int J Syst Evol Microbiol 53:1759–1777 [View Article][PubMed]
     [Google Scholar]
  116. Sohn H. J., Kim J. H., Shin M. H., Song K. J., Shin H. J. 2010; The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri . Parasitol Res 106:917–924 [View Article][PubMed]
     [Google Scholar]
  117. Song K. J., Song K. H., Na B. K., Kim J. H., Kwon D., Park S., Pak J. H., Im K. I., Shin H. J. 2007; Molecular cloning and characterization of a cytosolic heat shock protein 70 from Naegleria fowleri . Parasitol Res 100:1083–1089 [View Article][PubMed]
     [Google Scholar]
  118. Song K. J., Song K. H., Kim J. H., Sohn H. J., Lee Y. J., Park C. E., Shin H. J. 2008; Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity. Parasitol Res 103:313–317 [View Article][PubMed]
     [Google Scholar]
  119. Sood A., Chauhan S., Chandel L., Jaryal S. C. 2014; Prompt diagnosis and extraordinary survival from Naegleria fowleri meningitis: a rare case report. Indian J Med Microbiol 32:193–196 [View Article][PubMed]
     [Google Scholar]
  120. Sugita Y., Fujii T., Hayashi I., Aoki T., Yokoyama T., Morimatsu M., Fukuma T., Takamiya Y. 1999; Primary amebic meningoencephalitis due to Naegleria fowleri: an autopsy case in Japan. Pathol Int 49:468–470 [View Article][PubMed]
     [Google Scholar]
  121. Tiewcharoen S., Komalamisra N., Junnu V. 2004; Zymogram patterns of Naegleria spp isolated from natural water sources in Taling Chan district, Bangkok. Southeast Asian J Trop Med Publ Health 35:275–280
     [Google Scholar]
  122. Tiphine M., Letscher-Bru V., Herbrecht R. 1999; Amphotericin B and its new formulations: pharmacologic characteristics, clinical efficacy, and tolerability. Transpl Infect Dis 1:273–283 [View Article][PubMed]
     [Google Scholar]
  123. Trabelsi H., Dendana F., Sellami A., Sellami H., Cheikhrouhou F., Neji S., Makni F., Ayadi A. 2012; Pathogenic free-living amoebae: epidemiology and clinical review. Pathol Biol 60:399–405 [View Article][PubMed]
     [Google Scholar]
  124. Tyndall R. L., Domingue E. L. 1982; Cocultivation of Legionella pneumophila and free-living amoebae. Appl Environ Microbiol 44:954–959[PubMed]
     [Google Scholar]
  125. Valenzuela G., López-Corella E., De Jonckheere J. F. 1984; Primary amoebic meningoencephalitis in a young male from northwestern Mexico. Trans R Soc Trop Med Hyg 78:558–559 [View Article][PubMed]
     [Google Scholar]
  126. Vargas-Zepeda J., Gómez-Alcalá A. V., Vásquez-Morales J. A., Licea-Amaya L., De Jonckheere J. F., Lares-Villa F. 2005; Successful treatment of Naegleria fowleri meningoencephalitis by using intravenous amphotericin B, fluconazole and rifampicin. Arch Med Res 36:83–86 [View Article][PubMed]
     [Google Scholar]
  127. Visvesvara G. S., Moura H., Schuster F. L. 2007; Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea . FEMS Immunol Med Microbiol 50:1–26 [View Article][PubMed]
     [Google Scholar]
  128. Visvesvara G. S. 2013; Infections with free-living amebae. Handb Clin Neurol 114:153–168 [View Article][PubMed]
     [Google Scholar]
  129. Wang A., Kay R., Poon W. S., Ng H. K. 1993; Successful treatment of amoebic meningoencephalitis in a Chinese living in Hong Kong. Clin Neurol Neurosurg 95:249–252 [View Article][PubMed]
     [Google Scholar]
  130. WHO 2009 www.who.int/topics/epidemiology/en/
  131. Yadav D., Aneja S., Dutta R., Maheshwari A., Seth A. 2013; Youngest survivor of Naegleria meningitis. Indian J Pediatr 80:253–254 [View Article][PubMed]
     [Google Scholar]
  132. Yoder J. S., Eddy B. A., Visvesvara G. S., Capewell L., Beach M. J. 2010; The epidemiology of primary amoebic meningoencephalitis in the USA, 1962–2008. Epidemiol Infect 138:968–975 [View Article][PubMed]
     [Google Scholar]
  133. Yoder J. S., Straif-Bourgeois S., Roy S. L., Moore T. A., Visvesvara G. S., Ratard R. C., Hill V. R., Wilson J. D., Linscott A. J. et al. 2012; Primary amebic meningoencephalitis deaths associated with sinus irrigation using contaminated tap water. Clin Infect Dis 55:e7985 [View Article][PubMed]
     [Google Scholar]
  134. Yousuf F. A., Siddiqui R., Subhani F., Khan N. A. 2013; Status of free-living amoebae (Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris) in drinking water supplies in Karachi, Pakistan. J Water Health 11:371–375 [View Article][PubMed]
     [Google Scholar]
  135. Zhang L., Marciano-Cabral F., Bradley S. G. 1988; Effects of cyclophosphamide and a metabolite, acrolein, on Naegleria fowleri in vitro and in vivo . Antimicrob Agents Chemother 32:962–965 [View Article]
     [Google Scholar]
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Naegleria fowleri after 50 years: is it a neglected pathogen?
J. Med. Microbiol. 65, 885 (2016); https://doi.org/10.1099/jmm.0.000303
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