1887

Abstract

We present the first evaluation of a novel molecular assay, the Speed-Oligo complex (SO-MTBC), which is based on PCR combined with a dipstick for the differentiation of complex (MTBC) members. The results of this assay were compared with findings obtained using the Genotype MTBC assay. In this study, 189 strains of MTBC isolates from 2011 to 2014 were evaluated to determine the MTBC species. Most (174, 92 %) of the strains were identified as , 7 (3.7 %) as , 5 (2.6 %) as bacillus Calmette–Guérin, 2 (1.1 %) as and 1 (0.5 %) as ; no strains belonged to and subsp. The concordance κ coefficient obtained was 0.96 with the results of the Genotype MTBC assay. SO-MTBC may represent a fast and easy-to-use alternative for differentiating among MTBC subspecies in laboratories with standard equipment.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000308
2016-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/65/9/910.html?itemId=/content/journal/jmm/10.1099/jmm.0.000308&mimeType=html&fmt=ahah

References

  1. Bouakaze C., Keyser C., de Martino S. J., Sougakoff W., Veziris N., Dabernat H., Ludes B. 2010; Identification and genotyping of Mycobacterium tuberculosis complex species by use of a SNaPshot Minisequencing-based assay. J Clin Microbiol 48:1758–1766 [View Article][PubMed]
    [Google Scholar]
  2. Brosch R., Gordon S., Marmiesse M., Brodin P., Buchrieser C., Eiglmeier K., Garnier T., Gutierrez C., Hewinson G. et al. 2002; A new evolutionary scenario for the Mycobacterium tuberculosis complex. Proc Natl Acad Sci U S A 99:3684–3689 [View Article][PubMed]
    [Google Scholar]
  3. Bruker Daltonics Inc. 2013 Standard Operating Procedure: Mycobacteria Extraction (MycoEX) Method (Version 2.0) Germany: Bremen;
    [Google Scholar]
  4. Bruker Daltonics Inc. 2014 Standard operating procedure: Mycobacteria extraction (MycoEX) method (version 3.0) Germany: Bremen;
    [Google Scholar]
  5. de Jong B. C., Antonio M., Gagneux S. 2010; Mycobacterium africanum-review of an important cause of human tuberculosis in West Africa. PLoS Negl Trop Dis 4:e744 [View Article][PubMed]
    [Google Scholar]
  6. Devallois A., Goh K. S., Rastogi N. 1997; Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species. J Clin Microbiol 35:2969–2973[PubMed]
    [Google Scholar]
  7. Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman B. W. (editors) 2012; Bergey’s Manual® of Systematic Bacteriology. The Actinobacteria, Part A and B, 2nd edn. Vol. 5 pp. XLVIII–2083 New York, NY: Springer-Verlag;
    [Google Scholar]
  8. Gordon S., Brosch R., Billault A., Garnier T., Eiglmeier K., Cole S. T. 1999; Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays. Mol Microbiol 32:643–655 [View Article]
    [Google Scholar]
  9. Halse T. A., Escuyer V. E., Musser K. A. 2011; Evaluation of a single-tube multiplex real-time PCR for differentiation of members of the Mycobacterium tuberculosis complex in clinical specimens. J Clin Microbiol 49:2562–2567 [View Article][PubMed]
    [Google Scholar]
  10. Herrera-León L., Pozuelo-Díaz R., Molina Moreno T., Valverde Cobacho A., Saiz Vega P., Jiménez Pajares M. S. 2009; Differentiation of species within the Mycobacterium tuberculosis complex by molecular techniques. Enferm Infecc Microbiol Clin 27:496–502 [View Article][PubMed]
    [Google Scholar]
  11. Huard R. C., Lazzarini L. C., Butler W. R., van Soolingen D., Ho J. L. 2003; PCR-based method to differentiate the subspecies of the Mycobacterium tuberculosis complex on the basis of genomic deletions. J Clin Microbiol 41:1637–1650 [View Article][PubMed]
    [Google Scholar]
  12. Kasai H., Ezaki T., Harayama S. 2000; Differentiation of phylogenetically related slowly growing mycobacteria by their gyrB sequences. J Clin Microbiol 38:301–308[PubMed]
    [Google Scholar]
  13. Köser C. U., Piñero-Fernández S., Niemann S., Summers D. K. 2011; The validity of Mycobacterium tuberculosis complex species identification. Comments on the article: “Differentiation of species within the Mycobacterium tuberculosis complex by molecular techniques”. Enferm Infecc Microbiol Clin 29, :320 [View Article][PubMed]
    [Google Scholar]
  14. Liébana E., Aranaz A., Francis B., Cousins D. 1996; Assessment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex. J Clin Microbiol 34:933–938[PubMed]
    [Google Scholar]
  15. Mostowy S., Onipede A., Gagneux S., Niemann S., Kremer K., Desmond E. P., Kato-Maeda M., Behr M. 2004; Genomic analysis distinguishes Mycobacterium africanum . J Clin Microbiol 42:3594–3599 [View Article][PubMed]
    [Google Scholar]
  16. Niemann S., Harmsen D., Rüsch-Gerdes S., Richter E. 2000; Differentiation of clinical Mycobacterium tuberculosis complex isolates by gyrB DNA sequence polymorphism analysis. J Clin Microbiol 38:3231–3234[PubMed]
    [Google Scholar]
  17. Okazaki T., Ebihara S., Takahashi H., Asada M., Sato A., Seki M., Ohto H., Sasaki H. 2005; Multiplex PCR-identified cutaneous tuberculosis evoked by Mycobacterium bovis BCG vaccination in a healthy baby. J Clin Microbiol 43:523–525 [View Article][PubMed]
    [Google Scholar]
  18. Parsons L. M., Brosch R., Cole S. T., Somoskövi A., Loder A., Bretzel G., Van Soolingen D., Hale Y. M., Salfinger M. 2002; Rapid and simple approach for identification of Mycobacterium tuberculosis complex isolates by PCR-based genomic deletion analysis. J Clin Microbiol 40:2339–2345[PubMed] [CrossRef]
    [Google Scholar]
  19. Prodinger W. M., Brandstätter A., Naumann L., Pacciarini M., Kubica T., Boschiroli M. L., Aranaz A., Nagy G., Cvetnic Z. et al. 2005; Characterization of Mycobacterium caprae isolates from Europe by mycobacterial interspersed repetitive unit genotyping. J Clin Microbiol 43:4984–4992 [View Article][PubMed]
    [Google Scholar]
  20. Reddington K., Zumla A., Bates M., van Soolingen D., Niemann S., Barry T., O’Grady J. 2012; SeekTB, a two-stage multiplex real-time-PCR-based method for differentiation of the Mycobacterium tuberculosis complex. J Clin Microbiol 50:2203–2206 [View Article][PubMed]
    [Google Scholar]
  21. Richter E., Weizenegger M., Rüsch-Gerdes S., Niemann S. 2003; Evaluation of genotype MTBC assay for differentiation of clinical Mycobacterium tuberculosis complex isolates. J Clin Microbiol 41:2672–2675[PubMed] [CrossRef]
    [Google Scholar]
  22. Richter E., Weizenegger M., Fahr A. M., Rüsch-Gerdes S. 2004; Usefulness of the GenoType MTBC assay for differentiating species of the Mycobacterium tuberculosis complex in cultures obtained from clinical specimens. J Clin Microbiol 42:4303–4306 [View Article][PubMed]
    [Google Scholar]
  23. Somoskovi A., Dormandy J., Rivenburg J., Pedrosa M., McBride M., Salfinger M. 2008; Direct comparison of the genotype MTBC and genomic deletion assays in terms of ability to distinguish between members of the Mycobacterium tuberculosis complex in clinical isolates and in clinical specimens. J Clin Microbiol 46:1854–1857 [View Article][PubMed]
    [Google Scholar]
  24. Ueyama M., Chikamatsu K., Aono A., Murase Y., Kuse N., Morimoto K., Okumura M., Yoshiyama T., Ogata H. et al. 2014; Sub-speciation of Mycobacterium tuberculosis complex from tuberculosis patients in Japan. Tuberculosis 94:15–19 [View Article][PubMed]
    [Google Scholar]
  25. Wayne L., Kubica G. 1986; The mycobacteria. In Bergey’s Manual of Systematic Bacteriology vol. 2 pp. 1435–1457 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  26. World Health Organization 2014 Global Tuberculosis Report 2014 Geneva:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000308
Loading
/content/journal/jmm/10.1099/jmm.0.000308
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error