1887
  1. Home
  2. Publications
  3. Journal of Medical Microbiology
  4. Volume 65, Issue 9
  5. Article

f Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis-related infertility in women

PDF
252.01 Kb
HTML
64.10 Kb
  • Authors: Shruti Menon1​ ,  Scott H. Stansfield1​ ,  Benignus Logan2​ ,  Jane S. Hocking3​ ,  Peter Timms4​ ,  Luk Rombauts5​ ,  John A. Allan6​ ,7​ , Wilhelmina M. Huston1​ ,6​ ,8​
    • VIEW AFFILIATIONS
    • 1​ 1​ Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia 2​ 2​ Griffith University and UC Health Clinical School, The Wesley Hospital, Auchenflower, Queensland 4066, Australia 3​ 3​ Centre for Epidemiology and Biostatistics, Melbourne School of Population Health, University of Melbourne, Carlton 3053, Australia 4​ 4​ Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia 5​ 5​ MIMR-PH Institute of Medical Research, Monash, Victoria, Australia 6​ 6​ Wesley Medical Research, The Wesley Hospital, Auchenflower, Queensland 4066, Australia 7​ 7​ UC Health Clinical School, The Wesley Hospital, Auchenflower, Queensland 4066, Australia 8​ 8​ School of Life Sciences, Faculty of Science, University of Technology Sydney, Broadway, New South Wales 2007, Australia
  • Correspondence Wilhelmina M. Huston [email protected] or [email protected]
  • First Published Online: 01 September 2016, Journal of Medical Microbiology 65: 915-922, doi: 10.1099/jmm.0.000311
  • Subject: Clinical Microbiology
  • Received:
  • Accepted:
  • Cover date:
Preview this:
Free Preview thumbnail Magnify
Zoom in
Zoomout

Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis-related infertility in women, Page 1 of 1

| /docserver/preview/fulltext/jmm/65/9/915_jmm000311-1.gif

  • Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without subsequent surgical investigation during the infertility investigation. This study aimed to develop a highly specific serological assay for chlamydial tubal factor infertility in women that could be used to recommend direct progression to in vitro fertilization (IVF) treatment for women who are positive. Women were recruited from a variety of settings including women seeking fertility treatment, sexual health and general practitioner (GP) consultations or antenatal care (n=259). The serological assay was developed using sera from a large group of women by using infertile microimmunofluorescence (MIF)-positive women with tubal damage as the positives compared to infertile or acute infection and/or fertile controls (negatives). The new multi-peptide ELISA was highly specific for the detection of tubal factor infertility (P=0.011) compared to another ELISA (P=0.022) and MIF (P=0.099). The sensitivity of the assay should be improved before clinical utility. Potentially, a two-step testing protocol could be used during the initial infertility investigation, where MIF followed by a highly specific ELISA could be used to recommend direct progression to IVF for women who are positive.

  • Four supplementary tables are available with the online Supplementary Material.

  • Abbreviations:
    HREC Human Research Ethics Committee
    HRP horseradish peroxidase
    IVF in vitro fertilization
    MIF microimmunofluorescence
    ROC receiver operating characteristic
    STI sexually transmitted infection

© 2016 | Published by the Microbiology Society

  • Akande V. A. , Hunt L. P. , Cahill D. J. , Caul E. O. , Ford W. C. , Jenkins J. M. . ( 2003;). Tubal damage in infertile women: prediction using chlamydia serology. . Hum Reprod 18: 1841––1847. [CrossRef] [PubMed]
    [Google Scholar]
  • Broeze K. A. , Opmeer B. C. , Coppus S. F. , Van Geloven N. , Alves M. F. , Anestad G. , Bhattacharya S. , Allan J. , Guerra-Infante M. F. et al. ( 2011;). Chlamydia antibody testing and diagnosing tubal pathology in subfertile women: an individual patient data meta-analysis. . Hum Reprod Update 17: 301––310. [CrossRef] [PubMed]
    [Google Scholar]
  • Buderer N. M. . ( 1996;). Statistical methodology: I. Incorporating the prevalence of disease into the sample size calculation for sensitivity and specificity. . Acad Emerg Med 3: 895––900. [CrossRef] [PubMed]
    [Google Scholar]
  • Claman P. , Honey L. , Peeling R. W. , Jessamine P. , Toye B. . ( 1997;). The presence of serum antibody to the chlamydial heat shock protein (CHSP60) as a diagnostic test for tubal factor infertility. . Fertil Steril 67: 501––504. [CrossRef] [PubMed]
    [Google Scholar]
  • Coppus S. F. , Land J. A. , Opmeer B. C. , Steures P. , Eijkemans M. J. , Hompes P. G. , Bossuyt P. M. , Van der Veen F. , Mol B. W. , Van der Steeg J. W. . ( 2011;). Chlamydia trachomatis IgG seropositivity is associated with lower natural conception rates in ovulatory subfertile women without visible tubal pathology. . Hum Reprod 26: 3061––3067. [CrossRef] [PubMed]
    [Google Scholar]
  • Forsbach-Birk V. , Simnacher U. , Pfrepper K. I. , Soutschek E. , Kiselev A. O. , Lampe M. F. , Meyer T. , Straube E. , Essig A. . ( 2010;). Identification and evaluation of a combination of chlamydial antigens to support the diagnosis of severe and invasive Chlamydia trachomatis infections. . Clin Microbiol Infect 16: 1237––1244. [CrossRef] [PubMed]
    [Google Scholar]
  • Gijsen A. P. , Land J. A. , Goossens V. J. , Leffers P. , Bruggeman C. A. , Evers J. L. . ( 2001;). Chlamydia pneumoniae and screening for tubal factor subfertility. . Hum Reprod 16: 487––491. [CrossRef] [PubMed]
    [Google Scholar]
  • Keltz M. D. , Gera P. S. , Moustakis M. . ( 2006;). Chlamydia serology screening in infertility patients. . Fertil Steril 85: 752––754. [CrossRef] [PubMed]
    [Google Scholar]
  • Keltz M. D. , Sauerbrun-Cutler M. T. , Durante M. S. , Moshier E. , Stein D. E. , Gonzales E. . ( 2013;). Positive Chlamydia trachomatis serology result in women seeking care for infertility is a negative prognosticator for intrauterine pregnancy. . Sex Transm Dis 40: 842––845. [CrossRef] [PubMed]
    [Google Scholar]
  • Land J. A. , Gijsen A. P. , Kessels A. G. , Slobbe M. E. , Bruggeman C. A. . ( 2003;). Performance of five serological chlamydia antibody tests in subfertile women. . Hum Reprod 18: 2621––2627. [CrossRef] [PubMed]
    [Google Scholar]
  • Mehanna M. T. , Rizk M. A. , Eweiss N. Y. , Ramadan M. , Zaki S. A. , Sadek A. , Chow J. M. , Schachter J. . ( 1995;). Chlamydial serology among patients with tubal factor infertility and ectopic pregnancy in Alexandria, Egypt. . Sex Transm Dis 22: 317––321. [CrossRef] [PubMed]
    [Google Scholar]
  • Meikle S. F. , Zhang X. , Marine W. M. , Calonge B. N. , Hamman R. F. , Betz G. . ( 1994;). Chlamydia trachomatis antibody titers and hysterosalpingography in predicting tubal disease in infertility patients. . Fertil Steril 62: 305––312. [CrossRef] [PubMed]
    [Google Scholar]
  • Menon S. , Timms P. , Allan J. A. , Alexander K. , Rombauts L. , Horner P. , Keltz M. , Hocking J. , Huston W. M. . ( 2015;). Human and pathogen factors associated with Chlamydia trachomatis-related infertility in women. . Clin Microbiol Rev 28: 969––985. [CrossRef] [PubMed]
    [Google Scholar]
  • Mueller B. A. , Luz-Jimenez M. , Darling J. R. , Moore D. E. , McKnight B. , Weiss N. S. . ( 1991;). Risk factors for tubal infertility; influence of history of prior pelvic inflammatory disease. . Sex Transm Dis 19: 28––34.[CrossRef]
    [Google Scholar]
  • Oakeshott P. , Kerry S. , Aghaizu A. , Atherton H. , Hay S. , Taylor-Robinson D. , Simms I. , Hay P. . ( 2010;). Randomised controlled trial of screening for Chlamydia trachomatis to prevent pelvic inflammatory disease: the POPI (prevention of pelvic infection) trial. . BMJ 340: c1642. [CrossRef] [PubMed]
    [Google Scholar]
  • Persson K. , Osser S. , Birkelund S. , Christiansen G. , Brade H. . ( 1999;). Antibodies to Chlamydia trachomatis heat shock proteins in women with tubal factor infertility are associated with prior infection by C. trachomatis but not by C. pneumoniae . . Hum Reprod 14: 1969––1973. [CrossRef] [PubMed]
    [Google Scholar]
  • Price M. J. , Ades A. E. , Welton N. J. , Macleod J. , Turner K. , Simms I. , Horner P. J. . ( 2012;). How much tubal factor infertility is caused by Chlamydia? Estimates based on serological evidence corrected for sensitivity and specificity. . Sex Transm Dis 39: 608––613. [CrossRef] [PubMed]
    [Google Scholar]
  • Rodgers A. K. , Wang J. , Zhang Y. , Holden A. , Berryhill B. , Budrys N. M. , Schenken R. S. , Zhong G. . ( 2010;). Association of tubal factor infertility with elevated antibodies to Chlamydia trachomatis caseinolytic protease P. . Am J Obstet Gynecol 203: 494 e7––94 e14. [CrossRef] [PubMed]
    [Google Scholar]
  • Rodgers A. K. , Budrys N. M. , Gong S. , Wang J. , Holden A. , Schenken R. S. , Zhong G. . ( 2011;). Genome-wide identification of Chlamydia trachomatis antigens associated with tubal factor infertility. . Fertil Steril 96: 715––721. [CrossRef] [PubMed]
    [Google Scholar]
  • Sanchez-Campillo M. , Bini L. , Comanducci M. , Raggiaschi R. , Marzocchi B. , Pallini V. , Ratti G. . ( 1999;). Identification of immunoreactive proteins of Chlamydia trachomatis by Western blot analysis of a two-dimensional electrophoresis map with patient sera. . Electrophoresis 20: 2269––2279. [CrossRef] [PubMed]
    [Google Scholar]
  • Sexually transmitted diseases surveillence ( 2014;). http://www.cdc.gov/std/stats14/. Accessed 30/11/2015.
  • Stansfield S. H. , Patel P. , Debattista J. , Armitage C. W. , Cunningham K. , Timms P. , Allan J. , Mittal A. , Huston W. M. . ( 2013;). Proof of concept: a bioinformatic and serological screening method for identifying new peptide antigens for Chlamydia trachomatis related sequelae in women. . Results Immunol 3: 33––39. [CrossRef] [PubMed]
    [Google Scholar]
  • Swart P. , Mol B. W. , Van der Veen F. , Van Beurden M. , Redekop W. K. , Bossuyt P. M. . ( 1995;). The accuracy of hysterosalpingography in the diagnosis of tubal pathology: a meta-analysis. . Fertil Steril 64: 486––491. [CrossRef] [PubMed]
    [Google Scholar]
  • Toye B. , Laferrière C. , Claman P. , Jessamine P. , Peeling R. . ( 1993;). Association between antibody to the chlamydial heat-shock protein and tubal infertility. . J Infect Dis 168: 1236––1240. [CrossRef] [PubMed]
    [Google Scholar]
  • Wills G. S. , Horner P. J. , Reynolds R. , Johnson A. M. , Muir D. A. , Brown D. W. , Winston A. , Broadbent A. J. , Parker D. , McClure M. O. . ( 2009;). Pgp3 antibody enzyme-linked immunosorbent assay, a sensitive and specific assay for seroepidemiological analysis of Chlamydia trachomatis infection. . Clin Vaccine Immunol 16: 835––843. [CrossRef] [PubMed]
    [Google Scholar]
http://sgm.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000311
Loading

Supplementary Data

 Data loading....

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000311
2016-09-01
2019-02-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/65/9/915.html?itemId=/content/journal/jmm/10.1099/jmm.0.000311&mimeType=html&fmt=ahah

Author and Article Information

 Figure data loading....

Footnotes:

Validated antibodies in this article

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error