1887

Abstract

New Delhi metallo-β-lactamase (NDM)-producing (NPE) shows prevalence in China. Little is known about the mechanisms related to the spread of NPE. Recently, a total of 51 non-duplicated NPE isolates were collected from a tertiary-care hospital in China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility by Etest and sequence type by multilocus sequence typing. S1-PFGE and Southern blot analysis or PCR amplification were used for plasmid profiling. Between 2014 and 2015, 22 , 10 , 9 , 2 , 3 , 1 , 1 , 1 , 1 and 1 were identified as NPE. Results of PFGE and multilocus sequence typing showed that most strains were genetically unrelated. Among the 45 -carrying plasmids, there were 25 IncX3 plasmids with a size of about 30 to 50 kb, one 100 kb IncX3 plasmid, 11 IncA/C plasmids with a size range from 70 to 300 kb, six 90 to 120 kb IncB/O plasmids, one IncN plasmid with a size of 100 kb and one 140 kb IncFrep plasmid. An NDM-1-producing isolate of was identified, which had not been reported previously. An strain was found acquiring a -carrying IncFrep plasmid during infection. In conclusion, an NDM-1-producing isolate of was identified. An strain acquired a -carrying plasmid . IncX3 and IncA/C plasmids with various sizes might have emerged as the main platforms mediating the spread of the genes in China.

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2016-11-16
2024-03-29
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References

  1. Chen D., Gong L., Walsh T. R., Lan R., Wang T., Zhang J., Mai W., Ni N., Lu J. et al. 2016; Infection by and dissemination of NDM-5-producing Escherichia coli in China. J Antimicrob Chemother 71:563–565 [View Article][PubMed]
    [Google Scholar]
  2. Chmelnitsky I., Shklyar M., Hermesh O., Navon-Venezia S., Edgar R., Carmeli Y. 2013; Unique genes identified in the epidemic extremely drug-resistant KPC-producing Klebsiella pneumoniae sequence type 258. J Antimicrob Chemother 68:74–83 [View Article][PubMed]
    [Google Scholar]
  3. CLSI 2014 Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement (M100-S24) Vol. 34, No. 1. Wayne, PA 19087, USA Clinical and Laboratory Standards Institute
    [Google Scholar]
  4. Ho P. L., Li Z., Lo W. U., Cheung Y. Y., Lin C. H., Sham P. C., Cheng V. C., Ng T. K., Que T. L., Chow K. H. 2012; Identification and characterization of a novel incompatibility group X3 plasmid carrying blaNDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. Emerg Microbes Infect 1:e39 [View Article][PubMed]
    [Google Scholar]
  5. Hu L., Zhong Q., Tu J., Xu Y., Qin Z., Parsons C., Zhang B., Hu X., Wang L. et al. 2013; Emergence of blaNDM-1 among Klebsiella pneumoniae ST15 and novel ST1031 clinical isolates in China. Diagn Microbiol Infect Dis 75:373–376 [View Article][PubMed]
    [Google Scholar]
  6. Jin Y., Shao C., Li J., Fan H., Bai Y., Wang Y., Fan Y. B. 2015; Outbreak of multidrug resistant NDM-1-producing Klebsiella pneumoniae from a neonatal unit in Shandong Province, China. PLoS One 10:e0119571 [View Article][PubMed]
    [Google Scholar]
  7. Johnson T. J., Wannemuehler Y. M., Johnson S. J., Logue C. M., White D. G., Doetkott C., Nolan L. K. 2007; Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Appl Environ Microbiol 73:1976–1983 [View Article][PubMed]
    [Google Scholar]
  8. Johnson T. J., Bielak E. M., Fortini D., Hansen L. H., Hasman H., Debroy C., Nolan L. K., Carattoli A. 2012; Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae. Plasmid 68:43–50 [View Article][PubMed]
    [Google Scholar]
  9. Kumarasamy K. K., Toleman M. A., Walsh T. R., Bagaria J., Butt F., Balakrishnan R., Chaudhary U., Doumith M., Giske C. G. et al. 2010; Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis 10:597–602 [View Article][PubMed]
    [Google Scholar]
  10. Li J., Lan R., Xiong Y., Ye C., Yuan M., Liu X., Chen X., Yu D., Liu B. et al. 2014; Sequential isolation in a patient of Raoultella planticola and Escherichia coli bearing a novel ISCR1 element carrying blaNDM-1. PLoS One 9:e89893 [View Article][PubMed]
    [Google Scholar]
  11. Liu C., Qin S., Xu H., Xu L., Zhao D., Liu X., Lang S., Feng X., Liu H. M. 2015; New Delhi metallo-β-lactamase 1 (NDM-1), the dominant carbapenemase detected in carbapenem-resistant Enterobacter cloacae from Henan Province, China. PLoS One 10:e0135044 [View Article][PubMed]
    [Google Scholar]
  12. Liu Z., Li W., Wang J., Pan J., Sun S., Yu Y., Zhao B., Ma Y., Zhang T. et al. 2013; Identification and characterization of the first Escherichia coli strain carrying NDM-1 gene in China. PLoS One 8:e66666 [View Article][PubMed]
    [Google Scholar]
  13. Nordmann P., Dortet L., Poirel L. 2012; Carbapenem resistance in Enterobacteriaceae: here is the storm. Trends Mol Med 18:263–272 [View Article][PubMed]
    [Google Scholar]
  14. Qin S., Fu Y., Zhang Q., Qi H., Wen J. G., Xu H., Xu L., Zeng L., Tian H. et al. 2014; High incidence and endemic spread of NDM-1-positive Enterobacteriaceae in Henan Province, China. Antimicrob Agents Chemother 58:4275–4282 [View Article]
    [Google Scholar]
  15. Qin S., Qi H., Zhang Q., Zhao D., Liu Z. Z., Tian H., Xu L., Xu H., Zhou M. et al. 2015; Emergence of extensively drug-resistant Proteus mirabilis harboring a conjugative NDM-1 plasmid and a novel Salmonella genomic island 1 variant, SGI1-Z. Antimicrob Agents Chemother 59:6601–6604 [View Article][PubMed]
    [Google Scholar]
  16. Qu H., Wang X., Ni Y., Liu J., Tan R., Huang J., Li L., Sun J. 2015; NDM-1-producing Enterobacteriaceae in a teaching hospital in Shanghai, China: IncX3-type plasmids may contribute to the dissemination of blaNDM-1. Int J Infect Dis 34:8–13 [View Article][PubMed]
    [Google Scholar]
  17. Sun F., Yin Z., Feng J., Qiu Y., Zhang D., Luo W., Yang H., Yang W., Wang J. et al. 2015; Production of plasmid-encoding NDM-1 in clinical Raoultella ornithinolytica and Leclercia adecarboxylata from China. Front Microbiol 6:458 [View Article][PubMed]
    [Google Scholar]
  18. Tzouvelekis L. S., Markogiannakis A., Psichogiou M., Tassios P. T., Daikos G. L. 2012; Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions. Clin Microbiol Rev 25:682–707 [View Article][PubMed]
    [Google Scholar]
  19. Wailan A. M., Paterson D. L. 2014; The spread and acquisition of NDM-1: a multifactorial problem. Expert Rev Anti Infect Ther 12:91–115 [View Article][PubMed]
    [Google Scholar]
  20. Wang X., Xu X., Li Z., Chen H., Wang Q., Yang P., Zhao C., Ni M., Wang H. 2014; An outbreak of a nosocomial NDM-1-producing Klebsiella pneumoniae ST147 at a teaching hospital in mainland China. Microb Drug Resist 20:144–149 [View Article][PubMed]
    [Google Scholar]
  21. Wang X., Chen G., Wu X., Wang L., Cai J., Chan E. W., Chen S., Zhang R. 2015; Increased prevalence of carbapenem resistant Enterobacteriaceae in hospital setting due to cross-species transmission of the blaNDM-1 element and clonal spread of progenitor resistant strains. Front Microbiol 6:595 [View Article][PubMed]
    [Google Scholar]
  22. Yang J., Ye L., Guo L., Zhao Q., Chen R., Luo Y., Chen Y., Tian S., Zhao J. et al. 2013; A nosocomial outbreak of KPC-2-producing Klebsiella pneumoniae in a Chinese hospital: dissemination of ST11 and emergence of ST37, ST392 and ST395. Clin Microbiol Infect 19:E509E515 [View Article][PubMed]
    [Google Scholar]
  23. Yang P., Xie Y., Feng P., Zong Z. 2014; BlaNDM-5 carried by an IncX3 plasmid in Escherichia coli sequence type 167. Antimicrob Agents Chemother 58:7548–7552 [View Article][PubMed]
    [Google Scholar]
  24. Yang Q., Fang L., Fu Y., Du X., Shen Y., Yu Y. 2015; Dissemination of NDM-1-producing Enterobacteriaceae mediated by the IncX3-type plasmid. PLoS One 10:e0129454 [View Article][PubMed]
    [Google Scholar]
  25. Zhang X., Li X., Wang M., Yue H., Li P., Liu Y., Cao W., Yao D., Liu L. et al. 2015; Outbreak of NDM-1-producing Klebsiella pneumoniae causing neonatal infection in a teaching hospital in mainland China. Antimicrob Agents Chemother 59:4349–4351 [View Article][PubMed]
    [Google Scholar]
  26. Zheng R., Zhang Q., Guo Y., Feng Y., Liu L., Zhang A., Zhao Y., Yang X., Xia X. 2016; Outbreak of plasmid-mediated NDM-1-producing Klebsiella pneumoniae ST105 among neonatal patients in Yunnan, China. Ann Clin Microbiol Antimicrob 15: [View Article][PubMed]
    [Google Scholar]
  27. Zhou G., Guo S., Luo Y., Ye L., Song Y., Sun G., Guo L., Chen Y., Han L. et al. 2014; NDM-1-producing strains, family Enterobacteriaceae, in hospital, Beijing, China. Emerg Infect Dis 20:340–342 [View Article][PubMed]
    [Google Scholar]
  28. Zhou T., Zhang Y., Li M., Yu X., Sun Y., Xu J., Yu Y. S. 2015; An outbreak of infections caused by extensively drug-resistant Klebsiella pneumoniae strains during a short period of time in a Chinese teaching hospital: epidemiology study and molecular characteristics. Diagn Microbiol Infect Dis 82:240–244 [View Article][PubMed]
    [Google Scholar]
  29. Zhu J., Ding B., Xu X., Zhu D., Yang F., Zhang H., Hu F. 2015; Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia. Front Microbiol 6:1399 [View Article][PubMed]
    [Google Scholar]
  30. Zhu J., Sun L., Ding B., Yang Y., Xu X., Liu W., Zhu D., Yang F., Zhang H., Hu F. 2016; Outbreak of NDM-1-producing Klebsiella pneumoniae ST76 and ST37 isolates in neonates. Eur J Clin Microbiol Infect Dis 35:611–618 [View Article][PubMed]
    [Google Scholar]
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