1887

Abstract

The ultrastructural alterations caused by polymyxin B and meropenem and by the association between polymyxin B and meropenem were investigated in two multiresistant isolates of (K3-A2 and K12-A2) carriers of , coming from infection and colonization in patients in a public hospital in Recife, Brazil. The ultrastructural changes were detected by transmission electron microscopy and scanning. The susceptibility of the isolates to antimicrobials was tested by the disc diffusion method and microdilution in broth. The analysis by electron microscopy showed that the isolates presented morphological and ultrastructural cellular changes when subjected to a clinically relevant concentration of antimicrobials alone or in combination. When subjected to meropenem, they presented retraction of the cytoplasmic material, rupture of the cell wall and extravasation of the cytoplasmic content. When submitted to polymyxin B, the isolates showed condensation of the ribosomes, DNA clotting, cell wall thickening and the presence of membrane compartment. When subjected to polymyxin B and meropenem in combination, the isolates showed a higher intensity of the ultrastructural changes visualized. This is the first report of the ultrastructural changes caused by polymyxin B and meropenem in multiresistant isolates of carriers of the gene. It should be noted that even when the isolates were multiresistant carriers of the gene, they underwent important structural change owing to the action of polymyxin B and meropenem.

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2016-12-16
2024-03-28
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References

  1. ANVISA (Agência Nacional de Vigilância Sanitária), nota técnica n° 01/2013 Measures for the prevention and control of enterobacterial infections Multiresistant on April 17, 2013
  2. Bassetti M., Merelli M., Temperoni C., Astilean A. 2013; New antibiotics for bad bugs: where are we?. Ann Clin Microbiol Antimicrob 12:22 [View Article][PubMed]
    [Google Scholar]
  3. Brayner F. A., Araújo H. R. C., Cavalcanti M. G. S., Alves L. C., Peixoto C. A. 2005; Ultrastructural characterization of the hemocytes of Culex quinquefasciatus (Diptera: Culicidae). Micron 36:359367 [View Article][PubMed]
    [Google Scholar]
  4. Buijs J., Dofferhoff A. S., Mouton J. W., Wagenvoort J. H., van der Meer J. W. 2008; Concentration-dependency of β-lactam-induced filament formation in Gram-negative bacteria. Clin Microbiol Infect 14:344349 [View Article][PubMed]
    [Google Scholar]
  5. Cabral A. B., Melo R. C. A., Maciel M. A. V., Lopes A. C. S. 2012; Multidrug resistance genes, including blaKPC and blaCTX-M-2, among Klebsiella pneumoniae isolated in Recife, Brazil. Rev Soc Bras Med Trop 45:572578 [View Article][PubMed]
    [Google Scholar]
  6. Campos M. A., Vargas M. A., Regueiro V., Llompart C. M., Alberti S., Bengoechea J. A. 2004; Capsule polysaccharide mediates bacterial resistance to antimicrobial peptides. Infect Immun 72:71077114 [View Article][PubMed]
    [Google Scholar]
  7. CLSI 2014 Performance Standards for Antimicrobial Susceptibility Testing Twenty-fourth Informational Supplement, M100-S24 Wayne, PA: Clinical Laboratory Standards Institute;
    [Google Scholar]
  8. Dimech G. S., Soares L. A., Ferreira M. A., de Oliveira A. G., Carvalho M., Ximenes E. A. C. 2013; Phytochemical and antibacterial investigations of the extracts and fractions from the stem bark of Hymenaea stigonocarpa mart. ex Hayne and effect on ultrastructure of Staphylococcus aureus induced by hydroalcoholic extract. Sci World J 14:862763862771
    [Google Scholar]
  9. Dubrovskaya Y., Chen T. Y., Scipione M. R., Esaian D., Phillips M. S., Papadopoulos J., Mehta S. A. 2013; Risk factors for treatment failure of polymyxin B monotherapy for carbapenem-resistant Klebsiella pneumoniae infections. Antimicrob Agents Chemother 57:53945397 [View Article][PubMed]
    [Google Scholar]
  10. Jain R., Walk S. T., Aronoff D. M., Young V. B., Newton D. W., Chenoweth C. E., Washer L. L. 2013; Emergence of carbapenemase-producing Klebsiella pneumoniae of sequence type 258 in Michigan, USA. Infect Dis Rep 5:520 [View Article]
    [Google Scholar]
  11. Lee G. C., Burgessc D. S. 2013; Polymyxins and doripenem combination against KPC-producing Klebsiella pneumoniae . J Clin Med Res 5:97100 [View Article][PubMed]
    [Google Scholar]
  12. Lin M. Y., Lyles-Banks R. D., Lolans K., Hines D. W., Spear J. B., Petrak R., Trick W. E., Weinstein R. A., Hayden M. K. 2013; The importance of long-term acute care hospitals in the regional epidemiology of Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae. Centers for Disease Control and Prevention Epicenters Program. Diagn Microbiol Infect Dis 77:169173
    [Google Scholar]
  13. Lolans K., Calvert K., Won S., Clark J., Hayden M. K. 2010; Direct Ertapenem disk screening method for identification of KPC-producing Klebsiella pneumoniae and Escherichia coli in surveillance swab specimens. J Clin Microbiol 48:836841 [View Article][PubMed]
    [Google Scholar]
  14. Melo R. C. A., Barros E. M. R., Loureiro N. G., Melo H. R., Maciel M. A. V, Lopes A. C. S. 2014; Presence of fimH, mrkD, and irp2 virulence genes in KPC-2-producing Klebsiella pneumoniae isolates in Recife-PE, Brazil. Current Microbiology 69:824831
    [Google Scholar]
  15. Mendes C. A. C., Burdmann E. A. 2009; Polymyxins – a review focusing on their nephrotoxicity. Rev Assoc Med Bras 55:752759
    [Google Scholar]
  16. Moffatt J. H., Harper M., Harrison P., Hale J. D. F., Vinogradov E., Seemann T., Henry R., Crane B., St. Michael F. et al. 2010; Colistin resistance in Acinetobacter baumannii is mediated by complete loss of lipopolysaccharide production. Antimicrob Agents Chemother 54:49714977 [View Article]
    [Google Scholar]
  17. Munoz-Price L. S., Poirel L., Bonomo R. A., Schwaber M. J., Daikos G. L., Cormican M., Cornaglia G., Garau J., Gniadkowski M. et al. 2013; Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases. Lancet Infect Dis 13:785796 [View Article][PubMed]
    [Google Scholar]
  18. Olaitan A. O., Morand S., Rolain J. M. 2014; Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria. Front Microbiol 5:118 [View Article][PubMed]
    [Google Scholar]
  19. Padilla E., Llobet E., Doménech-Sánchez A., Martínez-Martínez L., Bengoechea J. A., Albertí S. 2010; Klebsiella pneumoniae AcrAB efflux pump contributes to antimicrobial resistance and virulence. Antimicrob Agents Chemother 54:177183 [View Article][PubMed]
    [Google Scholar]
  20. Papadimitriou-Olivgeris M., Marangos M., Fligou F., Christofidou M., Sklavou C., Vamvakopoulou S., Anastassiou E. D., Filos K. S. 2013; KPC-producing Klebsiella pneumoniae enteric colonization acquired during intensive care unit stay: the significance of risk factors for its development and its impact on mortality. Diagn Microbiol Infect Dis 77:169173 [View Article][PubMed]
    [Google Scholar]
  21. Romano B., Pagano E., Montanaro V., Fortunato A. L., Milic N., Borrelli F. 2013; Novel insights into the pharmacology of flavonoids. Phytother Res 27:15881596 [View Article][PubMed]
    [Google Scholar]
  22. Santhana Raj L., Hing H. L., Baharudin O., Teh Hamidah Z., Aida Suhana R., Nor Asiha C. P., Vimala B., Paramsarvaran S., Sumarni G., Hanjeet K. 2007; Mesosomes are a definite event in antibiotic-treated Staphylococcus aureus ATCC 25923. Trop Biomed 24:105109[PubMed]
    [Google Scholar]
  23. Tang S. S., Apisarnthanarak A., Hsu L. Y. 2014; Mechanisms of β-lactam antimicrobial resistance and epidemiology of major community- and healthcare-associated multidrug-resistant bacteria. Adv Drug Deliv Rev 78:313 [View Article][PubMed]
    [Google Scholar]
  24. Walther-Rasmussen J., Høiby N. 2007; Class A carbapenemases. J Antimicrob Chemother 60:470482 [View Article][PubMed]
    [Google Scholar]
  25. Whitfield C., Naismith J. H. 2008; Periplasmic export machines for outer membrane assembly. Curr Opin Struct Biol 18:466474 [View Article][PubMed]
    [Google Scholar]
  26. Yigit H., Queenan A. M., Anderson G. J., Domenech-Sanchez A., Biddle J. W., Steward C. D., Alberti S., Bush K., Tenover F. C. 2001; Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother 45:11511161 [View Article][PubMed]
    [Google Scholar]
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