RT Journal Article SR Electronic(1) A1 Brum, Alexandre Antunes A1 Rezende, Andrea de Fatima Silva A1 Brilhante, Francisco Silvestre A1 Collares, Thais A1 Begnine, Karine A1 Seixas, Fabiana Kommling A1 Collares, Tiago Veiras A1 Dellagostin, Odir Antônio A1 Azevedo, Vasco A1 Santos, Anderson A1 Portela, Ricardo Wagner A1 Borsuk, SibeleYR 2017 T1 Recombinant esterase from Corynebacterium pseudotuberculosis in DNA and subunit recombinant vaccines partially protects mice against challenge JF Journal of Medical Microbiology, VO 66 IS 5 SP 635 OP 642 DO https://doi.org/10.1099/jmm.0.000477 PB Microbiology Society, SN 1473-5644, AB Purpose. We tested the efficacy of the esterase encoded by cp1002_RS09720 from Corynebacterium pseudotuberculosis in recombinant subunit and DNA caseous lymphadenitis (CLA) vaccines. This target was predicted as one of the best CLA vaccine candidates by mature epitope density analysis. Methodology. Gene cp1002_RS09720 was cloned into two different vectors (pAE for subunit vaccine and pTARGET for DNA vaccine). Four groups of 15 mice each were immunized with the recombinant esterase rCP09720 associated with aluminium hydroxide adjuvant (G1), pTARGET/cp09720 DNA vaccine (G2), a naked pTARGET (G3) or PBS as a negative control (G4). Immunization occurred in two doses intercalated by a 21 day interval. Twenty-one days after the last dose administration, animals were challenged with a virulent C. pseudotuberculosis MIC-6 strain. Results. G1 showed high levels of IgG1 and IgG2a on days 21 and 42 post-immunization and a significant level of IFN-γ (P<0.05), suggesting a Th1 response. The protection levels obtained were 58.3 and 16.6 % for G1 and G2, respectively. Conclusion. The subunit vaccine composed of the recombinant esterase rCP09720 and Al(OH)3 is a promising antigenic formulation for use against CLA., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000477