First description of methyltransferases in extensively drug-resistant Klebsiella pneumoniae isolates from Saudi Arabia
Abdalhamid, Baha and Elhadi, Nasreldin and Albunayan, Samar and Alsamman, Khaldoon and Aljindan, Reem,, 66, 859-863 (2017),
doi = https://doi.org/10.1099/jmm.0.000480,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= Purpose. The resistance determinants for carbapenems, fluoroquinolones and aminoglycosides were characterized in 16 extensively drug-resistant Klebsiella pneumoniae (XDRKPN) strains collected from Saudi hospitals during 2014. Methodology. PCR and sequencing were used to detect: bla KPC, bla NDM, bla VIM, bla IMP-1, bla OXA-48, bla CTX-M, bla TEM, bla SHV and amp C for β-lactam resistance; qnr A, qnr B, qnrS, aac(6′)-Ib-cr, qep A and mutations of gyr A and par C for fluoroquinolone resistance; and aac A4, aac C2, aad A1, aph A6, arm A and rmt B for aminoglycoside resistance. Enterobacterial repetitive intergenic consensus sequence-based PCR was performed to detect the clonal relatedness. Results. All isolates encoded bla CTX-M, aac C2 and aph A6, together with mutations in gyr A and par C. b la OXA-48, bla NDM-1, aad A1, aac A4, qnr B, aac(6′)-Ib-cr, arm A and/or rmt B were detected in different strains. At least 93.2 % clonal relatedness was detected among these strains. Conclusions. To our knowledge, this is the first report describing XDRKPN encoding at least seven resistance determinants and harbouring methyltransferases in Saudi Arabia.,
language=,
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