1887

Abstract

We investigated the transcription of adhesin-encoding genes , and in strain J99. Each possesses a repeating homopolymeric nucleotide tract within their promoter regions, and and possess repeats within their 5′ coding regions.

We altered the repeat lengths associated with the adhesin genes and quantified mRNA levels by real-time quantitative PCR. Using adherence to AGS cells and IL-8 assays, we examined the effects of altered transcript levels. We assessed the role of ArsRS in transcription using an null mutant and by examining ArsR binding to promoter regions via electrophoretic mobility shift assays.

Extensions or truncations of promoter region repeats in and increased transcript levels, mirroring results shown by our lab and others for mutations in the promoter. Altered lengths of the poly-cytosine thymine tract within the 5′ coding region of demonstrated that switching from phase-off to phase-on significantly increased mRNA levels. However, mutations in the poly-thymine tract of , which increased mRNA levels, do not behave synergistically with phase-on mutations. Phase-on mutations of resulted in increased adherence to AGS cells, but only a modest effect on IL-8. and , and paralogue transcript levels were increased in an mutant and ArsR bound the promoter regions for each of these genes .

This work highlights the complex nature of adhesin regulation, its impact on attachment and the pervasive role of ArsRS in adhesin expression. Such regulation may help facilitate the decades-long persistence of infection.

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2017-06-01
2024-03-29
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