%0 Journal Article %A Sardi, Janaina de Cássia Orlandi %A Polaquini, Carlos Roberto %A Freires, Irlan Almeida %A Galvão, Livia Câmara de Carvalho %A Lazarini, Josy Goldoni %A Torrezan, Guilherme Silva %A Regasini, Luis Octávio %A Rosalen, Pedro Luiz %T Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion %D 2017 %J Journal of Medical Microbiology, %V 66 %N 6 %P 816-824 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000494 %K diacetylcurcumin %K anti-adhesion %K antibiofilm %K Staphylococcus aureus %K curcumin %K toxicity %I Microbiology Society, %X Purpose. Staphylococcus aureus infections have contributed to the global healthcare burden, particularly with regard to hospital-acquired meticillin-resistant S. aureus (MRSA) infections.Methodology. This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae.Results. DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 µmol l−1, and minimum bactericidal concentration ranging between 69 and 277 µmol l−1. It significantly reduced bacterial biofilm survival – by 22–63 % (at MIC, 10×MIC or 100×MIC) as compared to the 25–42 % reduction by vancomycin (P<0.0001) – and severely affected biofilm cell structures, leading to damaged architecture and the formation of amorphous cell clusters. Treatment with DAC (MIC/4) decreased bacterial adhesion to HaCaT keratinocytes from 1 to 5 h (P<0.0001). Finally, DAC demonstrated low toxicity in G. mellonella at its effective anti-biofilm concentrations.Conclusion. These findings open new avenues for the study of this curcumin derivative as an excellent prototype with anti-MRSA activity. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000494