1887

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Volume 66, Issue 7

Characterization of high level ampicillin- and aminoglycoside-resistant enterococci isolated from non-hospital sources Free

Abstract

High level ampicillin- and aminoglycoside-resistant enterococci are being increasingly reported from non-hospital sources. This study was carried out to characterize these strains from non-hospital sources in Nigeria.

A collection of isolated from vegetables, soil, farm animals and manure and observed to be resistant to ampicillin (=63) and gentamicin (=37) discs, were screened for resistance to high levels of ampicillin and aminoglycoside using E-test strips. Putative high level ampicillin- and aminoglycoside-resistant strains were screened for 5 and aminoglycoside modifying enzyme genes, respectively, by PCR. The C-terminal region of the amplified 5 gene was also sequenced.

Five (5/63) and thirty-five (35/37) of the ampicillin- and aminoglycoside-resistant strains were identified as high level ampicillin- and aminoglycoside-resistant strains, respectively, based on the MIC results. The amplified 5 gene from the high level ampicillin-resistant isolates displayed 96–99 % nucleotide sequence similarity with the reference strains and three novel insertions (500Glu→Leu, 502Asp→Arg and 614Ile→Phe) in the amino acid sequence. Aminoglycoside modifying enzyme genes ″) (100 %), (88.8 %), (90 %) and (40 %) were detected among the high level aminoglycoside-resistant isolates.

This is the first report on the characterization of high level ampicillin- and aminoglycoside-resistant among animals and vegetables in Nigeria. The results show that non-hospital sources can constitute a reservoir for potential dissemination of these strains and genes to humans via the food chain or by direct contact.

  • Received:
  • Accepted:
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Keyword(s): Enterococcus faecium , high level aminoglycoside resistance , high level ampicillin resistance , Nigeria , non-human sources and vegetables
© 2017 The Authors
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2017-07-01
2024-04-19
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Characterization of high level ampicillin- and aminoglycoside-resistant enterococci isolated from non-hospital sources
J. Med. Microbiol. 66, 1027 (2017); https://doi.org/10.1099/jmm.0.000518
/content/journal/jmm/10.1099/jmm.0.000518
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