1887

Abstract

The present study aimed to characterize cell damage caused by vaginal isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and 4 from women with recurrent vulvovaginal candidiasis (RVVC). We evaluated the ability of to adhere to human cervical cancer cells (SiHa), the yeast–SiHa cell interactions and cell damage. All of the clinical isolates presented a high adhesion capacity on SiHa cells. However, clinical isolates from symptomatic women (VVC and RVVC) had higher filamentation after contact (24 h) with SiHa cells and a greater capacity to cause cell damage (>80 %). Clinical isolates from symptomatic women had greater potential to invade SiHa cells, suggesting that they are more pathogenic than AS isolates.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000547
2017-08-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/8/1225.html?itemId=/content/journal/jmm/10.1099/jmm.0.000547&mimeType=html&fmt=ahah

References

  1. Sobel JD. Vulvovaginal candidosis. Lancet 2007; 369:1961–1971 [View Article][PubMed]
    [Google Scholar]
  2. Hasan F, Xess I, Wang X, Jain N, Fries BC. Biofilm formation in clinical Candida isolates and its association with virulence. Microbes Infect 2009; 11:753–761 [View Article][PubMed]
    [Google Scholar]
  3. Barousse MM, van der Pol BJ, Fortenberry D, Orr D, Fidel PL. Vaginal yeast colonisation, prevalence of vaginitis, and associated local immunity in adolescents. Sex Transm Infect 2004; 80:48–53 [View Article][PubMed]
    [Google Scholar]
  4. Workowski KA, Bolan GA. Centers for Disease Control and Prevention Sexually transmitted diseases treatment guidelines. MMWR Recomm Rep 2015; 64:75–78
    [Google Scholar]
  5. Ferraza M, Maluf MLF, Consolaro MEL, Shinobu CS, Svidzinski TIE et al. Characterization of yeasts isolated from the vagina and their association with vulvovaginal candidíasis in two cities of the South of Brazil. Rev Bras Ginecol Obstet 2005; 27:58–63 [CrossRef]
    [Google Scholar]
  6. Gunther LS, Martins HP, Gimenes F, Abreu AL, Consolaro ME et al. Prevalence of Candida albicans and non-albicans isolates from vaginal secretions: comparative evaluation of colonization, vaginal candidiasis and recurrent vaginal candidiasis in diabetic and non-diabetic women. Sao Paulo Med J 2014; 132:116–120 [View Article][PubMed]
    [Google Scholar]
  7. Taguti Irie MM, Lopes Consolaro ME, Aparecida Guedes T, Donatti L, Valéria Patussi E et al. A simplified technique for evaluating the adherence of yeasts to human vaginal epithelial cells. J Clin Lab Anal 2006; 20:195–203 [View Article][PubMed]
    [Google Scholar]
  8. Alves CT, Silva S, Pereira L, Williams DW, Azeredo J et al. Effect of progesterone on Candida albicans vaginal pathogenicity. Int J Med Microbiol 2014; 304:1011–1017 [View Article][PubMed]
    [Google Scholar]
  9. Ratti BA, Godoy JS, de Souza Bonfim Mendonça P, Bidóia DL, Nakamura TU et al. Microbicidal activity of neutrophils is inhibited by isolates from recurrent vaginal candidiasis (RVVC) caused by Candida albicans through fungal thioredoxin reductase. Cell Immunol 2015; 293:22–29 [View Article][PubMed]
    [Google Scholar]
  10. Akimoto-Gunther L, Bonfim-Mendonça PS, Takahachi G, Irie MM, Miyamoto S et al. Highlights regarding host predisposing factors to recurrent vulvovaginal candidiasis: chronic stress and reduced antioxidant capacity. PLoS One 2016; 11:e0158870 [View Article][PubMed]
    [Google Scholar]
  11. Giolo MP, Svidzinski TIE. Epidemiology and laboratory diagnosis of candidemia. J Bras Pato Med Lab 2010; 46:225–234 [CrossRef]
    [Google Scholar]
  12. Khan MSA, Ahmad I, Aqil F, Owais M, Shahid M et al. Virulence and pathogenicity of fungal pathogens with special reference to Candida albicans. Combating Fungal Infections Springer; 2010 pp. 21–45 [CrossRef]
    [Google Scholar]
  13. Karkowska-Kuleta J, Rapala-Kozik M, Kozik A. Fungi pathogenic to humans: molecular bases of virulence of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. Acta Biochim Pol 2009; 56:211–224[PubMed]
    [Google Scholar]
  14. Soll DR. Candida biofilms: is adhesion sexy?. Curr Biol 2008; 18:R717–R720 [View Article][PubMed]
    [Google Scholar]
  15. Grubb SE, Murdoch C, Sudbery PE, Saville SP, Lopez-Ribot JL et al. Candida albicans-endothelial cell interactions: a key step in the pathogenesis of systemic candidiasis. Infect Immun 2008; 76:4370–4377 [View Article][PubMed]
    [Google Scholar]
  16. Wilson D, Naglik JR, Hube B. The missing link between Candida albicans Hyphal Morphogenesis and Host Cell Damage. PLoS Pathog 2016; 12:e1005867 [View Article][PubMed]
    [Google Scholar]
  17. Rast TJ, Kullas AL, Southern PJ, Davis DA. Human epithelial cells discriminate between commensal and pathogenic interactions with Candida albicans. PLoS One 2016; 11:e0153165 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000547
Loading
/content/journal/jmm/10.1099/jmm.0.000547
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error