High frequency of the combined presence of QRDR mutations and PMQR determinants in multidrug-resistant Klebsiella pneumoniae and Escherichia coli isolates from nosocomial and community-acquired infections

Bruna Fuga Araújo; Paola Amaral de Campos; Sabrina Royer; Melina Lorraine Ferreira; Iara Rossi Gonçalves; Deivid William da Fonseca Batistão; Daiane Silva Resende; Cristiane Silveira de Brito; Paulo Pinto Gontijo-Filho; Rosineide Marques Ribas

doi:10.1099/jmm.0.000551

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f High frequency of the combined presence of QRDR mutations and PMQR determinants in multidrug-resistant Klebsiella pneumoniae and Escherichia coli isolates from nosocomial and community-acquired infections

Authors: Bruna Fuga Araújo¹ , Paola Amaral de Campos¹ , Sabrina Royer¹ , Melina Lorraine Ferreira¹ , Iara Rossi Gonçalves¹ , Deivid William da Fonseca Batistão² , Daiane Silva Resende³ , Cristiane Silveira de Brito¹ , Paulo Pinto Gontijo-Filho¹ , Rosineide Marques Ribas¹
- VIEW AFFILIATIONS
- ¹ 1Institute of Biomedical Sciences (ICBIM), Laboratory of Molecular Microbiology, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil ² 2School of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil ³ 3Faculty of João Pinheiro City, Minas Gerais, Brazil
*Correspondence: Bruna Fuga Araújo, [email protected]
First Published Online: 04 August 2017, Journal of Medical Microbiology 66: 1144-1150, doi: 10.1099/jmm.0.000551
Subject: Clinical Microbiology

Received: 28/03/2017
Accepted: 30/06/2017
Cover date: 01/08/2017

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Plasmid-mediated quinolone resistance (PMQR) determinants combined with mutations in quinolone resistance-determining regions (QRDRs) and clonal dissemination were investigated in 40 fluoroquinolone-resistant Klebsiella pneumoniae and Escherichia coli isolates from nosocomial and community-acquired infections. We observed nucleotide substitutions in gyrA (Ser83Ile, Val37Leu, Lys154Arg, Ser171Ala, Ser19Asn, Ile198Val, Ser83Tyr, Ser83Leu, Asp87Asn and Asp87Gly) and parC genes (Ser80Ile, Glu84Lys, Ala129Ser, Val141Ala and Glu84Gly). Two novel substitutions were detected in the gyrA gene (Val37Leu and Ile198Val). The presence of PMQR genes predominated in community isolates (55.5 %). In addition to the frequent presence of the class 1 integron in isolates from community-acquired infections, the genetic similarity results obtained by PFGE showed high genomic diversity. This study suggests that management of multidrug-resistant Enterobacteriaceae isolates from the community are a possible source of genetic mobile elements that carry genes that confer resistance to fluoroquinolones. More attention should be paid to the surveillance of community-acquired infections.

One supplementary table and one supplementary figure are available with the online Supplementary Material.

Keyword(s): multidrug-resistance, plasmid-mediated quinolone resistance (PMQR), Escherichia coli, Klebsiella pneumoniae, quinolone resistance determining regions (QRDR) mutations

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