@article{mbs:/content/journal/jmm/10.1099/jmm.0.000551, author = "Araújo, Bruna Fuga and Campos, Paola Amaral de and Royer, Sabrina and Ferreira, Melina Lorraine and Gonçalves, Iara Rossi and Batistão, Deivid William da Fonseca and Resende, Daiane Silva and Brito, Cristiane Silveira de and Gontijo-Filho, Paulo Pinto and Ribas, Rosineide Marques", title = "High frequency of the combined presence of QRDR mutations and PMQR determinants in multidrug-resistant Klebsiella pneumoniae and Escherichia coli isolates from nosocomial and community-acquired infections", journal= "Journal of Medical Microbiology", year = "2017", volume = "66", number = "8", pages = "1144-1150", doi = "https://doi.org/10.1099/jmm.0.000551", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000551", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "Escherichia coli", keywords = "quinolone resistance determining regions (QRDR) mutations", keywords = "multidrug-resistance", keywords = "plasmid-mediated quinolone resistance (PMQR)", keywords = "Klebsiella pneumoniae", abstract = "Plasmid-mediated quinolone resistance (PMQR) determinants combined with mutations in quinolone resistance-determining regions (QRDRs) and clonal dissemination were investigated in 40 fluoroquinolone-resistant Klebsiella pneumoniae and Escherichia coli isolates from nosocomial and community-acquired infections. We observed nucleotide substitutions in gyrA (Ser83Ile, Val37Leu, Lys154Arg, Ser171Ala, Ser19Asn, Ile198Val, Ser83Tyr, Ser83Leu, Asp87Asn and Asp87Gly) and parC genes (Ser80Ile, Glu84Lys, Ala129Ser, Val141Ala and Glu84Gly). Two novel substitutions were detected in the gyrA gene (Val37Leu and Ile198Val). The presence of PMQR genes predominated in community isolates (55.5 %). In addition to the frequent presence of the class 1 integron in isolates from community-acquired infections, the genetic similarity results obtained by PFGE showed high genomic diversity. This study suggests that management of multidrug-resistant Enterobacteriaceae isolates from the community are a possible source of genetic mobile elements that carry genes that confer resistance to fluoroquinolones. More attention should be paid to the surveillance of community-acquired infections.", }