Evaluation of the Cobas u 701 microscopy analyser compared with urine culture in screening for urinary tract infection

Si Hyun Kim; Sae Am Song; Sang-Hwa Urm; Joong-Ki Kook; Hye Ran Kim; Dongeun Yong; Jun Yong Choi; Jeong Hwan Shin

doi:10.1099/jmm.0.000553

Tools

Add to My Favorites
You must be logged in to use this functionality
Create Content Alert

Create Correction Alert
Export citations
- BibT_EX
- Endnote
- Zotero
- Plain Text
- RefWorks
- Mendeley
Recommend to library

You must fill out fields marked with: *

Librarian details Name: *

Email: *

Your details Name: *

Email: *

Department: *

Why are you recommending this title?

Select reason:
I need to refer to this publication frequently

This publication is an essential resource for my studies/research

I'll refer my students to this publication

I'm an author/editor/contributor to this publication

I'm a member of the publication's editorial board

Other:

eventtype:PERSONALISATION;jsessionid:_FzVMqq4rVEGy9f1ddQ6u1X9.x-sgm-live-02;itemid:http://sgm.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000553;timestamp:1550425976432

Invalid site public key

Invalid site private key

Invalid request cookie

Incorrect. Try again.

Unabled to verify parameters

Could not contact recaptcha for validation

I am not a robot *

776794535

Journal of Medical Microbiology — Recommend this title to your library

Thank you
Your recommendation has been sent to your librarian.
Request Permission
Order Reprint

f Evaluation of the Cobas u 701 microscopy analyser compared with urine culture in screening for urinary tract infection

Authors: Si Hyun Kim^1,2,† , Sae Am Song^1,† , Sang-Hwa Urm³ , Joong-Ki Kook⁴ , Hye Ran Kim¹ , Dongeun Yong⁵ , Jun Yong Choi⁶ , Jeong Hwan Shin^1,2
- VIEW AFFILIATIONS
- ¹ 1Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea ² 2Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Republic of Korea ³ 3Department of Preventive Medicine, Inje University College of Medicine, Busan, Republic of Korea ⁴ 4Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea ⁵ 5Department of Laboratory Medicine and Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, Seoul, Republic of Korea ⁶ 6Division of Infectious Disease, Department of Internal Medicine and AIDS Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
*Correspondence: Jeong Hwan Shin, [email protected]
First Published Online: 04 August 2017, Journal of Medical Microbiology 66: 1110-1113, doi: 10.1099/jmm.0.000553
Subject: Clinical Microbiology

Received: 28/03/2017
Accepted: 02/07/2017
Cover date: 01/08/2017

Preview this:

Evaluation of the Cobas u 701 microscopy analyser compared with urine culture in screening for urinary tract infection, Page 1 of 1

< Previous page | Next page > /docserver/preview/fulltext/jmm/66/8/1110_jmm000553-1.gif

Purpose. A new automated Cobas u 701 microscopy analyser for urine sediment examination was introduced. The aim of this study was to evaluate the analyser in comparison with urine culture in screening for urinary tract infection (UTI).

Methodology. A total of 852 urine specimens submitted for culture were included in this study. Urine sediment examination was performed using the Cobas u 701 microscopy analyser. The results of the bacteria (BAC) and yeast (YEA) analyses were compared with the results from urine culture as a method for UTI screening. In addition, we compared the BAC results with white blood cells (WBCs) and leukocyte and nitrite measurement in the Cobas u 601 system.

Results. Of the 852 urine specimens, 16.1 % (N=137) were positive by urine culture, yielding 130 bacteria from 124 specimens and 14 yeasts from 14 specimens. The Cobas u 701 microscopy analyser provided no result for 52 specimens because of their high turbidity. The sensitivity, specificity, positive predictive value and negative predictive value were 85.8, 69.4, 33.1 and 96.5 %, respectively. For YEA, these figures were 100, 91.9, 15.8 and 100 %, respectively. The areas under the curve for BAC and WBCs were 0.827 [95 % confidence interval (CI) 0.799, 0.852] and 0.727 (95 % CI 0.695, 0.757), respectively. The sensitivity of the leukocyte and nitrite was 63.5 and 54.6 %, respectively.

Conclusion. The Cobas u 701 microscopy analyser showed good diagnostic performance. It can be used for rapid screening for UTI and can reduce the number of cultures required.

†
These authors contributed equally to this work.

Keyword(s): Cobas u 701, urine culture, urinary tract infection

1. Finkelstein R, Kassis E, Reinhertz G, Gorenstein S, Herman P. Community-acquired urinary tract infection in adults: a hospital viewpoint. J Hosp Infect 1998;38:193–202 [CrossRef][PubMed]
[Google Scholar]
2. Kim Z, Song KE, Lee W-K. A case of Trichosporon asahii Fungemia with urinary tract infection as a primary focus. Ann Clin Microbiol 2015;18:56–59 [CrossRef]
[Google Scholar]
3. Stalenhoef JE, van Dissel JT, van Nieuwkoop C. Febrile urinary tract infection in the emergency room. Curr Opin Infect Dis 2015;28:106–111 [CrossRef][PubMed]
[Google Scholar]
4. Johansson C, Molander U, Milsom I, Ekelund P. Association between urinary incontinence and urinary tract infections, and fractures in postmenopausal women. Maturitas 1996;23:265–271 [CrossRef][PubMed]
[Google Scholar]
5. Foxman B. Recurring urinary tract infection: incidence and risk factors. Am J Public Health 1990;80:331–333 [CrossRef][PubMed]
[Google Scholar]
6. Broeren MA, Bahçeci S, Vader HL, Arents NL. Screening for urinary tract infection with the Sysmex UF-1000i urine flow cytometer. J Clin Microbiol 2011;49:1025–1029 [CrossRef][PubMed]
[Google Scholar]
7. Hsiao C-Y, Yang H-Y, Chang C-H, Lin H-L, Wu C-Y et al. Risk factors for development of septic shock in patients with urinary tract infection. Biomed Res Int 2015;2015:1–7 [CrossRef]
[Google Scholar]
8. Kim Y, Park KG, Lee K, Park YJ. Direct identification of urinary tract pathogens from urine samples using the Vitek MS system based on matrix-assisted laser desorption ionization-time of flight mass spectrometry. Ann Lab Med 2015;35:416–422 [CrossRef][PubMed]
[Google Scholar]
9. Gutiérrez-Fernández J, Riazzo C, Sanbonmatsu S, de Dios Luna J, Sorlózano A et al. Sysmex UF-1000i performance for screening yeasts in urine. APMIS 2014;122:324–328 [CrossRef][PubMed]
[Google Scholar]
10. Monsen T, Rydén P. Flow cytometry analysis using sysmex UF-1000i classifies uropathogens based on bacterial, leukocyte, and erythrocyte counts in urine specimens among patients with urinary tract infections. J Clin Microbiol 2015;53:539–545 [CrossRef][PubMed]
[Google Scholar]
11. Regeniter A, Haenni V, Risch L, Köchli HP, Colombo JP et al. Urine analysis performed by flow cytometry: reference range determination and comparison to morphological findings, dipstick chemistry and bacterial culture results – a multicenter study. Clin Nephrol 2001;55:384–392[PubMed]
[Google Scholar]
12. Kim SY, Kim YJ, Lee SM, Hwang SH, Kim HH et al. Evaluation of the Sysmex UF-100 urine cell analyzer as a screening test to reduce the need for urine cultures for community-acquired urinary tract infection. Am J Clin Pathol 2007;128:922–925 [CrossRef][PubMed]
[Google Scholar]
13. Koken T, Aktepe OC, Serteser M, Samli M, Kahraman A et al. Determination of cut-off values for leucocytes and bacteria for urine flow cytometer (UF-100) in urinary tract infections. Int Urol Nephrol 2002;34:175–178 [CrossRef][PubMed]
[Google Scholar]
14. Zaman Z, Roggeman S, Verhaegen J. Unsatisfactory performance of flow cytometer UF-100 and urine strips in predicting outcome of urine cultures. J Clin Microbiol 2001;39:4169–4171 [CrossRef][PubMed]
[Google Scholar]
15. Manoni F, Fornasiero L, Ercolin M, Tinello A, Ferrian M et al. Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections. Diagn Microbiol Infect Dis 2009;65:103–107 [CrossRef][PubMed]
[Google Scholar]
16. Pieretti B, Brunati P, Pini B, Colzani C, Congedo P et al. Diagnosis of bacteriuria and leukocyturia by automated flow cytometry compared with urine culture. J Clin Microbiol 2010;48:3990–3996 [CrossRef][PubMed]
[Google Scholar]
17. Kadkhoda K, Manickam K, Degagne P, Sokolowski P, Pang P et al. UF-1000i flow cytometry is an effective screening method for urine specimens. Diagn Microbiol Infect Dis 2011;69:130–136 [CrossRef][PubMed]
[Google Scholar]
18. Chien TI, Kao JT, Liu HL, Lin PC, Hong JS et al. Urine sediment examination: a comparison of automated urinalysis systems and manual microscopy. Clin Chim Acta 2007;384:28–34 [CrossRef][PubMed]
[Google Scholar]

http://sgm.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000553

Supplementary Data

Data loading....

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000553

2017-08-04

2019-02-17

Full text loading...

/deliver/fulltext/jmm/66/8/1110.html?itemId=/content/journal/jmm/10.1099/jmm.0.000553&mimeType=html&fmt=ahah

Author and Article Information

This Journal

/content/journal/jmm/10.1099/jmm.0.000553

dcterms_title,dcterms_subject,pub_serialTitle

pub_serialIdent:journal/jmm AND -contentType:BlogPost

10

4
Other Society Journals

/content/journal/jmm/10.1099/jmm.0.000553

dcterms_title,dcterms_subject

-pub_serialIdent:journal/jmm AND -contentType:BlogPost

10

4
PubMed
Google Scholar

Figure data loading....

f Evaluation of the Cobas u 701 microscopy analyser compared with urine culture in screening for urinary tract infection

Supplementary Data

Author and Article Information

This Journal

Other Society Journals

PubMed

Google Scholar

Footnotes:

Validated antibodies in this article