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Volume 66, Issue 11

CHROMagar COL-: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens Free

Abstract

A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant and spp. (CHROMagar COL- was developed, evaluated and compared to an existing selective bacterial culture medium (SuperPolymyxin).

The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples. Susceptibility to COL and POL B was determined by agar dilution and broth microtitre dilution. The lower limit for the detection of COL-resistant organisms was also calculated for both CHROMagar COL- and SuperPolymyxin media. The ability to isolate and correctly differentiate COL-resistant organisms within mixed cultures was also assessed and compared using both media.

Using CHROMagar COL- Gram-negative pathogens (=71) with intrinsic (=8) or acquired COL (=63) resistance were recovered with 100 % specificity down to the lower limit of detection of 10 colony-forming units (c.f.u.). The growth on SuperPolymyxin was similar, but notably weaker for COL-resistant non-fermentative bacteria ( and ). CHROMagar COL- was also more sensitive in supporting the growth of with COL resistance associated with the carriage of

CHROMagar COL- is a sensitive and specific medium for the growth of COL-resistant bacterial pathogens. Due to the low limit of detection (10 c.f.u.), it may be useful as a primary isolation medium in the surveillance and recovery of COL-resistant bacteria from complex human, veterinary and environmental samples, especially those with plasmid-mediated MCR-1 or novel mechanisms of polymyxin resistance.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CHROMagar COL-APSE , colistin , MCR-1 and resistance
© 2017 The Authors
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2017-11-01
2024-04-25
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References

  1. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016; 16:161–168 [View Article][PubMed]
     [Google Scholar]
  2. Nation RL, Li J, Cars O, Couet W, Dudley MN et al. Framework for optimisation of the clinical use of colistin and polymyxin B: the Prato polymyxin consensus. Lancet Infect Dis 2015; 15:225–234 [View Article][PubMed]
     [Google Scholar]
  3. Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Eurosurveillance 2016; 21:pii-30280 [View Article]
     [Google Scholar]
  4. Nordmann P, Jayol A, Poirel L. A universal culture medium for screening polymyxin-resistant gram-negative isolates. J Clin Microbiol 2016; 54:1395–1399 [View Article][PubMed]
     [Google Scholar]
  5. Clinical and Laboratory Standards Institute Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 9th ed. vol. 32 Wayne, Pennsylvania, USA: CLSI; 2012 pp. M07–A9
     [Google Scholar]
  6. Clinical and Laboratory Standards Institute Performance Standards For Antimicrobial Susceptibility Testing; supplement M100S , 25th ed. Wayne, Pennsylvania, USA: CLSI; 2015
     [Google Scholar]
  7. European Committee on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters. EUCAST 2017Version 7.1
     [Google Scholar]
  8. Miles AA, Misra SS, Irwin JO. The estimation of the bactericidal power of the blood. J Hyg 1938; 38:732–749 [View Article][PubMed]
     [Google Scholar]
  9. Jayol A, Nordmann P, Brink A, Poirel L. Heteroresistance to colistin in Klebsiella pneumoniae associated with alterations in the PhoPQ regulatory system. Antimicrob Agents Chemother 2015; 59:2780–2784 [View Article][PubMed]
     [Google Scholar]
  10. Bergen PJ, Forrest A, Bulitta JB, Tsuji BT, Sidjabat HE et al. Clinically relevant plasma concentrations of colistin in combination with imipenem enhance pharmacodynamic activity against multidrug-resistant Pseudomonas aeruginosa at multiple inocula. Antimicrob Agents Chemother 2011; 55:5134–5142 [View Article][PubMed]
     [Google Scholar]
  11. Phee L, Hornsey M, Wareham DW. In vitro activity of daptomycin in combination with low-dose colistin against a diverse collection of gram-negative bacterial pathogens. Eur J Clin Microbiol Infect Dis 2013; 32:1291–1294 [View Article][PubMed]
     [Google Scholar]
  12. Agersø Y, Torpdahl M, Zachariasen C, Seyfarth A, Hammerum AM et al. Tentative colistin epidemiological cut-off value for Salmonella spp. Foodborne Pathog Dis 2012; 9:367–369 [View Article][PubMed]
     [Google Scholar]
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CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens
J. Med. Microbiol. 66, 1554 (2017); https://doi.org/10.1099/jmm.0.000602
/content/journal/jmm/10.1099/jmm.0.000602
/content/journal/jmm/10.1099/jmm.0.000602
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