Modified use of real-time PCR detection of group B Streptococcus in pregnancy
Rabaan, Ali A. and Saunar, Justin V. and Bazzi, Ali M. and Soriano, Joan L.,, 66, 1516-1520 (2017),
doi = https://doi.org/10.1099/jmm.0.000604,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= The CDC recommends antenatal screening of vaginal/rectal samples for Streptococcus agalactiae at 35–37 weeks' gestation, with intra-partum antibiotic prophylaxis for positive cases. We tested a modified use of the Cepheid Xpert GBS real-time PCR kit on enrichment cultures from 554 vaginal/rectal swabs compared to the current subculturing gold standard method. Swabs were inoculated on polymyxin nalidixic acid agar plates, and Todd–Hewitt enrichment broth cultures were examined daily for growth. Todd–Hewitt broth culture was also used for Xpert GBS. There was 92.06 % agreement between the subculture and PCR methods. Sensitivity of Xpert GBS was 100 %, specificity was 89.40 %, positive predictive value was 75.96 % and negative predictive value was 100 %. Colonization incidence was higher with younger (≤24 years) or older (≥35 years) maternal age. Modified use of the Cepheid Xpert GBS would assist rapid diagnosis of S. agalactiae colonization and facilitate timely and appropriate assignment to intra-partum antibiotic prophylaxis.,
language=,
type=