1887

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Volume 67, Issue 3

Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning Free

Abstract

Micro-organisms are important triggers of peri-implant inflammation and analysing their diversity is necessary for peri-implantitis treatment. This study aimed to analyse and compare the microbiota associated with individuals with peri-implantitis, as well as clinically healthy implant sites.

Subgingival biofilm samples were taken from 10 individuals with peri-implantitis and from at least 1 clinically healthy implant. DNA was extracted and bacterial 16S rRNA genes were amplified using universal primers. After cloning the PCR-products, amplified inserts of positive clones were digested using restriction endonucleases, and the chosen clones were sequenced. The 16S rDNA-sequences were compared to those from the public sequence databases GenBank, EMBL and DDBJ to determine the corresponding taxa.

Differing distributions of taxa belonging to the phyla , , , , , , and TM 7 were detected in both the healthy implant (HI) and the peri-implantitis (PI) groups. A significantly higher relative abundance of phylum , as well as of the species were found in the PI group (<0.05). The putative periodontal red complex () was also detected at significantly higher levels in the PI group (<0.05), whereas the yellow group, as well as the species tended to be associated with the HI group.

A shift in the healthy subgingival microbiota was shown in peri-implantitis-associated biofilm. Anaerobic Gram-negative periopathogens, including and , seem to play an important role in peri-implantitis development and should be considered in treatment and prevention strategies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): culture-independent method , microbiota , peri-implant disease , subgingival biofilm and subgingival microbial complexes
© 2018 The Authors
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2018-03-01
2024-04-19
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Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning
J. Med. Microbiol. 67, 332 (2018); https://doi.org/10.1099/jmm.0.000682
/content/journal/jmm/10.1099/jmm.0.000682
/content/journal/jmm/10.1099/jmm.0.000682
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