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Volume 67, Issue 6

Identification and prevalence of RND family multidrug efflux pump AB genes in isolates from swine manure in China Free

Abstract

The resistance/nodulation/cell division (RND) family multidrug efflux pump, OqxAB, has been identified as one of the leading mechanisms of plasmid-mediated quinolone resistance and has become increasingly prevalent among in recent years. However, AB genes have not yet been reported in isolates. The aim of the present study was to identify the AB genes and investigate their prevalence among from swine manure in China.

The AB genes were screened in 87 isolates by PCR. The transferability of the AB genes in was determined by conjugation experiments. The genetic environment of AB genes was investigated by cloning experiments, PCR mapping and sequencing.

A high prevalence (86.2 %) of olaquindox resistance was observed in and 98.9 % isolates exhibited multidrug-resistance phenotypes. The occurrence of A and B in was also high (79.3 and 65.5 %, respectively). Sequence analysis of the cloned fragment indicated that the AB cassette was linked to an incomplete Tn5 transposon containing and flanked by IS [ISAB-IS]. The AB–-positive transconjugant or transformant showed resistance or reduced susceptibility to enrofloxacin, ciprofloxacin, olaquindox, mequindox, florfenicol, neomycin and kanamycin.

This is the first time that the AB genes have been identified in from swine manure. The genetic linkage of AB– in has not been described before. The high prevalence of AB genes in suggests that it may constitute a reservoir for AB genes and pose a potential threat to public health.

  • Received:
  • Accepted:
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Keyword(s): aph(3')-IIa , Enterococcus , Olaquindox resistance , oqxAB and RND multidrug efflux pump
© 2018 The Authors
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2018-06-01
2024-04-19
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Identification and prevalence of RND family multidrug efflux pump oqxAB genes in Enterococci isolates from swine manure in China
J. Med. Microbiol. 67, 733 (2018); https://doi.org/10.1099/jmm.0.000736
/content/journal/jmm/10.1099/jmm.0.000736
/content/journal/jmm/10.1099/jmm.0.000736
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