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Volume 67, Issue 6

Enterotoxin gene profile of methicillin-resistant isolates from dogs, humans and the environment Free

Abstract

This study aimed to detect and identify staphylococcal enterotoxin (SE) genes in methicillin-resistant (MRSP) strains from different sources, and to investigate the relationship between their sequence types (STs) and SE gene patterns.

The profiles of 17 SE genes in 93 MRSP strains isolated from dogs (=43), humans (=18) and the environment (=32) were detected by PCR. Multilocus sequence typing (MLST), SCC typing and pulsed-field gel electrophoresis (PFGE) were used to analyse the clonal relatedness between the molecular type and SE gene profiles.

The human MRSP strains harboured the greatest number of SE genes (12/17; and ) compared to those from dogs (5/17; and ) and environmental sources (3/17; and ). Using MLST and PFGE, different SE gene profiles were found within the same clonal type.

We show that isolates of MRSP vary in their virulence gene profiles, depending on the source from which they have been isolated. This insight should encourage the development of appropriate monitoring and mitigation strategies to reduce the transmission of MRSP in veterinary hospitals and households.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): dog , environment , human , Staphylococcal enterotoxins and Staphylococcus pseudintermedius
© 2018 The Authors
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2018-06-01
2024-04-19
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Enterotoxin gene profile of methicillin-resistant Staphylococcus pseudintermedius isolates from dogs, humans and the environment
J. Med. Microbiol. 67, 866 (2018); https://doi.org/10.1099/jmm.0.000748
/content/journal/jmm/10.1099/jmm.0.000748
/content/journal/jmm/10.1099/jmm.0.000748
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