
oa Accuracy of a commercial multiplex PCR for the diagnosis of bacterial vaginosis
- Authors: Charlotte van der Veer1 , Robin van Houdt2 , Alje van Dam1,3 , Henry de Vries1,4,5 , Sylvia Bruisten1,5,*
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1 1Department of Infectious Diseases, Public Health Service Amsterdam, Amsterdam, the Netherlands 2 2Department of Medical Microbiology and Infection Prevention, VU University Medical Center, Amsterdam, the Netherlands 3 3Department of Medical Microbiology, OLVG General Hospital, Amsterdam, the Netherlands 4 4Department of Dermatology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands 5 5Amsterdam Infection and Immunity Institute, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands
- *Correspondence: Sylvia Bruisten [email protected]
- First Published Online: 09 July 2018, Journal of Medical Microbiology 67: 1265-1270, doi: 10.1099/jmm.0.000792
- Subject: Disease, Diagnosis and Diagnostics
- Received:
- Accepted:
- Cover date:
- This is an open access article published by the Microbiology Society under the Creative Commons Attribution License




Accuracy of a commercial multiplex PCR for the diagnosis of bacterial vaginosis, Page 1 of 1
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Purpose. Bacterial vaginosis (BV) is a common clinical condition characterized by odorous vaginal discharge, vaginal itching and/or burning. BV can occur when vaginal lactobacilli are depleted and replaced by diverse anaerobic bacteria. We evaluated a commercial multiplex PCR (ATRiDA) for the diagnosis of BV.
Methods. Cervicovaginal samples were included from women reporting urogenital symptoms and from women notified for sexually transmitted infections (STI) – who were not (necessarily) symptomatic. Clinical BV diagnoses were obtained from electronic patient files. The ATRiDA test measures the loads of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus species in relation to overall bacterial load. The ATRiDA test outcome was compared to the clinical BV diagnosis and to vaginal microbiota composition, determined by 16SrRNA gene sequencing.
Results. We included samples from 185 women reporting urogenital symptoms, of whom 81 had BV and 93 women who were notified for an STI, of whom 16 had BV. Overall, compared to the clinical BV diagnosis, the ATRiDA test demonstrated high sensitivity (96.9 %) and moderate specificity (70.2 %). The negative predictive value was high (>97.3). The positive predictive value differed by study group and was highest in women reporting urogenital symptoms (78.2 %). Sequencing showed that 54 % of women who had an ATRiDA BV-positive test outcome, but who were not clinically diagnosed with BV, had diverse anaerobic vaginal microbiota (asymptomatic vaginal dysbiosis).
Conclusion. The ATRiDA test is a sensitive method for the detection of BV but, given the high occurrence of asymptomatic vaginal dysbiosis, a positive test outcome should be interpreted together with clinical symptoms.
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Three supplementary tables are available with the online version of this article.
- Keyword(s): multiplex PCR, bacterial vaginosis, vaginal microbiota, molecular diagnostics
© 2018 The Authors | Published by the Microbiology Society
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