1887

Abstract

Several species of Aeromonas produce the enzyme CphA metallo-β-lactamase. This study describes an isolate of Aeromonas hydrophila harbouring an acquired gene encoding the carbapenemase GES-24. This isolate was obtained from an inpatient in Okinawa, Japan, with no apparent record of travelling overseas. The minimum inhibitory concentrations of carbapenems against this isolate were 8 µg ml for imipenem and 16 µg ml for meropenem. Recombinant GES-24 hydrolyzed all of the tested β-lactams, including imipenem and meropenem. The genomic environment surrounding bla GES-24 was intI1-bla GES-24 -aac(6′)-IIc-qacEdelta1-sulI-orfX-tetR-tetE. This is the first report of A. hydrophila producing a GES-type carbapenemase.

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2018-10-05
2024-04-23
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References

  1. Parker JL, Shaw JG. Aeromonas spp. clinical microbiology and disease. J Infect 2011; 62:109–118 [View Article][PubMed]
    [Google Scholar]
  2. Rossolini GM, Zanchi A, Chiesurin A, Amicosante G, Satta G et al. Distribution of cphA or related carbapenemase-encoding genes and production of carbapenemase activity in members of the genus Aeromonas. Antimicrob Agents Chemother 1995; 39:346–349 [View Article][PubMed]
    [Google Scholar]
  3. Tada T, Shrestha B, Miyoshi-Akiyama T, Shimada K, Ohara H et al. NDM-12, a novel New Delhi metallo-β-lactamase variant from a carbapenem-resistant Escherichia coli clinical isolate in Nepal. Antimicrob Agents Chemother 2014; 58:6302–6305 [View Article][PubMed]
    [Google Scholar]
  4. Gomi R, Matsuda T, Yamamoto M, Chou PH, Tanaka M et al. Characteristics of carbapenemase-producing Enterobacteriaceae in wastewater revealed by genomic analysis. Antimicrob Agents Chemother 2018; 62:e0250102517 [View Article][PubMed]
    [Google Scholar]
  5. Rodríguez-Martínez JM, Machuca J, Cano ME, Calvo J, Martínez-Martínez L et al. Plasmid-mediated quinolone resistance: two decades on. Drug Resist Updat 2016; 29:13–29 [View Article][PubMed]
    [Google Scholar]
  6. Pawlowski AC, Stogios PJ, Koteva K, Skarina T, Evdokimova E et al. The evolution of substrate discrimination in macrolide antibiotic resistance enzymes. Nat Commun 2018; 9:112 [View Article][PubMed]
    [Google Scholar]
  7. Girlich D, Poirel L, Nordmann P. Diversity of clavulanic acid-inhibited extended-spectrum β-lactamases in Aeromonas spp. from the Seine River, Paris, France. Antimicrob Agents Chemother 2011; 55:1256–1261 [View Article][PubMed]
    [Google Scholar]
  8. Neuwirth C, Siebor E, Robin F, Bonnet R. First occurrence of an IMP metallo-beta-lactamase in Aeromonas caviae: IMP-19 in an isolate from France. Antimicrob Agents Chemother 2007; 51:4486–4488 [View Article][PubMed]
    [Google Scholar]
  9. Libisch B, Giske CG, Kovács B, Tóth TG, Füzi M. Identification of the first VIM metallo-beta-lactamase-producing multiresistant Aeromonas hydrophila strain. J Clin Microbiol 2008; 46:1878–1880 [View Article][PubMed]
    [Google Scholar]
  10. Hughes HY, Conlan SP, Lau AF, Dekker JP, Michelin AV et al. Detection and whole-genome sequencing of carbapenemase-producing Aeromonas hydrophila isolates from routine perirectal surveillance culture. J Clin Microbiol 2016; 54:1167–1170 [View Article][PubMed]
    [Google Scholar]
  11. Anandan S, Gopi R, Devanga Ragupathi NK, Muthuirulandi Sethuvel DP, Gunasekaran P et al. First report of blaOXA-181-mediated carbapenem resistance in Aeromonas caviae in association with pKP3-A: Threat for rapid dissemination. J Glob Antimicrob Resist 2017; 10:310–314 [View Article][PubMed]
    [Google Scholar]
  12. Montezzi LF, Campana EH, Corrêa LL, Justo LH, Paschoal RP et al. Occurrence of carbapenemase-producing bacteria in coastal recreational waters. Int J Antimicrob Agents 2015; 45:174–177 [View Article][PubMed]
    [Google Scholar]
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