%0 Journal Article %A Kottom, Theodore J. %A Hebrink, Deanne M. %A Limper, Andrew H. %T Binding of Pneumocystis carinii to the lung epithelial cell receptor HSPA5 (GRP78) %D 2018 %J Journal of Medical Microbiology, %V 67 %N 12 %P 1772-1777 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000864 %K Pneumocystis Epithelium Adherence Receptor %I Microbiology Society, %X The importance of lung macrophages in Pneumocystis–host interaction is well known, but little is known about the initial binding/colonization of the airway epithelium. Our prior studies have documented cell-signalling events that occur following binding of the organisms to lung epithelial cells; however, the receptors that mediate Pneumocystis attachment to lung surfaces have not yet been fully defined. Using affinity chromatography, we identified heat shock protein 5 (HSPA5), also known as GRP78, as a potential host receptor that may have relevance in Pneumocystis lung colonization. Pneumocystis carinii (Pc) organisms not only bound HSPA5 on a rat lung epithelial cell line, but also on primary rat airway epithelial cells (AECs). Furthermore, Pc bound CHO1 cells overexpressing HSPA5 more than the CHO1 parent line alone, supporting a role for Pc–HSPA5 protein interaction in mediating organism attachment. These results provide new insights into the interactions of Pneumocystis with host lung epithelium. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000864