1887

Abstract

In this study, we evaluated the Sofia Streptococcus pneumoniae FIA test (Quidel Corporation, San Diego, CA, USA), a new immunofluorescence-based lateral flow test for the qualitative detection of S. pneumoniae antigen in urine or cerebrospinal fluid specimens. The analyses of 100 non-concentrated urine samples (including 50 samples from S. pneumoniae cases) showed a sensitivity and specificity (95 % CI) of, respectively, 66.0 % (52.2–77.6) and 100.0 % (92.9–100.0) for the Sofia test, and 62.0 % (48.2–74.1) and 98.0 % (89.5–99.7) for the BinaxNOW SPN Antigen Card. There were no significant differences in sensitivity and specificity between the tests (McNemar’s tests, P=0.625 and P=1.000). In conclusion, this study indicates that the Streptococcus pneumoniae FIA test shows similar sensitivity and specificity rates compared to the BinaxNOW SPN Antigen Card.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000877
2018-11-07
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/67/12/1743.html?itemId=/content/journal/jmm/10.1099/jmm.0.000877&mimeType=html&fmt=ahah

References

  1. van der Poll T, Opal SM. Pathogenesis, treatment, and prevention of pneumococcal pneumonia. Lancet 2009; 374:1543–1556 [View Article][PubMed]
    [Google Scholar]
  2. Laupland KB, Church DL. Population-based epidemiology and microbiology of community-onset bloodstream infections. Clin Microbiol Rev 2014; 27:647–664 [View Article][PubMed]
    [Google Scholar]
  3. Sinclair A, Xie X, Teltscher M, Dendukuri N. Systematic review and meta-analysis of a urine-based pneumococcal antigen test for diagnosis of community-acquired pneumonia caused by Streptococcus pneumoniae. J Clin Microbiol 2013; 51:2303–2310 [View Article][PubMed]
    [Google Scholar]
  4. Mandell L. Prospective randomised study to compare empirical treatment versus targeted treatment on the basis of the urine antigen results in hospitalised patients with community-acquired pneumonia. Thorax 2010; 65:93–94 [View Article][PubMed]
    [Google Scholar]
  5. Yu VL, Stout JE. Rapid diagnostic testing for community-acquired pneumonia: can innovative technology for clinical microbiology be exploited?. Chest 2009; 136:1618–1621 [View Article][PubMed]
    [Google Scholar]
  6. Marcos MA, Jiménez de Anta MT, de La Bellacasa JP, González J, Martínez E et al. Rapid urinary antigen test for diagnosis of pneumococcal community-acquired pneumonia in adults. Eur Respir J 2003; 21:209–214 [View Article][PubMed]
    [Google Scholar]
  7. Vicente D, López-Olaizola M, de La Caba I, Cilla G. Evaluation of Sofia Fluorescent immunoassay analyzer for pneumococcal urinary antigen detection in hospitalized patients with community-acquired pneumonia. J Microbiol Methods 2017; 141:25–27 [View Article][PubMed]
    [Google Scholar]
  8. Watanabe H, Uruma T, Tazaki G, Kikuchi R, Tsuji T et al. Clinical factors associated with negative urinary antigen tests implemented for the diagnosis of community-acquired pneumococcal pneumonia in adult patients. Med Princ Pract 2015; 24:189–194 [View Article][PubMed]
    [Google Scholar]
  9. Rosón B, Fernández-Sabé N, Carratalà J, Verdaguer R, Dorca J et al. Contribution of a urinary antigen assay (Binax NOW) to the early diagnosis of pneumococcal pneumonia. Clin Infect Dis 2004; 38:222–226 [View Article][PubMed]
    [Google Scholar]
  10. Domínguez J, Galí N, Blanco S, Pedroso P, Prat C et al. Detection of Streptococcus pneumoniae antigen by a rapid immunochromatographic assay in urine samples. Chest 2001; 119:243–249 [View Article][PubMed]
    [Google Scholar]
  11. Gutiérrez F, Masiá M, Rodríguez JC, Ayelo A, Soldán B et al. Evaluation of the immunochromatographic Binax NOW assay for detection of Streptococcus pneumoniae urinary antigen in a prospective study of community-acquired pneumonia in Spain. Clin Infect Dis 2003; 36:286–292 [View Article][PubMed]
    [Google Scholar]
  12. Smith MD, Derrington P, Evans R, Creek M, Morris R et al. Rapid diagnosis of bacteremic pneumococcal infections in adults by using the Binax NOW Streptococcus pneumoniae urinary antigen test: a prospective, controlled clinical evaluation. J Clin Microbiol 2003; 41:2810–2813 [View Article][PubMed]
    [Google Scholar]
  13. Said MA, Johnson HL, Nonyane BA, Deloria-Knoll M, O'Brien KL et al. Estimating the burden of pneumococcal pneumonia among adults: a systematic review and meta-analysis of diagnostic techniques. PLoS One 2013; 8:e60273 [View Article][PubMed]
    [Google Scholar]
  14. Murdoch DR, Laing RT, Mills GD, Karalus NC, Town GI et al. Evaluation of a rapid immunochromatographic test for detection of Streptococcus pneumoniae antigen in urine samples from adults with community-acquired pneumonia. J Clin Microbiol 2001; 39:3495–3498 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000877
Loading
/content/journal/jmm/10.1099/jmm.0.000877
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error