1887

Abstract

. Herpes simplex virus (HSV) is a common lifelong sexually transmitted infection. HSV-1 typically manifests as oral cold sores, while HSV-2 is more traditionally associated with sexual transmission and infection. We have developed a real-time PCR (Trioplex) for the simultaneous detection of HSV-1 and -2 and the bacterial phage internal control (IC) MS2.

. To determine the performance of the Trioplex method and resolve discrepancies, 178 clinical specimens from cutaneous and mucocutaneous sources were tested using 3 different methods; virus culture with direct fluorescent antibody (DFA) immunostaining, Trioplex and a commercially available HSV analyte-specific reagent (ASR).

. HSV Trioplex was significantly more sensitive than virus culture (89 and 67 % HSV 1/2, respectively) and comparable to the commercial assay (<0.001). Cost analysis revealed that the Trioplex reduced cost by 80  % compared to cell culture.

. The implementation of the HSV Trioplex improved the detection turnaround time from 3–10 days to 2.5 h, thus streamlining Herpes detection, improving sensitivity and reducing laboratory costs.

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2019-05-01
2024-04-20
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