1887

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Volume 68, Issue 8

Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil Free

Abstract

Human bocavirus (HBoV) is a DNA virus that is mostly associated with respiratory infections. However, because it has been found in stool samples, it has been suggested that it may be a causative agent for human enteric conditions. This underpins the continuous search for HBoVs, especially after the introduction of the rotavirus vaccine due to acute gastroenteritis cases related to emergent viruses, as HBoVs are more likely to be found in this post-vaccine scenario. Therefore, the aim of this study is to demonstrate the prevalence of HBoV in children aged less than 10 years with acute gastroenteritis in Brazil from November 2011 to November 2012.

Stool samples from hospitalized children ≤10 years old who presented symptoms of acute gastroenteritis were analysed for the presence of rotavirus A (RVA) by an enzyme-linked immunosorbent assay (ELISA), and for HBoV DNA by nested PCR.

HBoV positivity was detected in 24.0 % (54/225) of samples. Two peaks of HBoV detection were observed in November 2011 and from July to September 2012. Co-infections between HBoV and rotavirus A were identified in 50.0 % (27/54) of specimens. Phylogenetic analysis identified the presence of HBoV-1 (94.8 %), HBoV-2 (2.6 %) and HBoV-3 (2.6 %) species, with only minor variations among them.

Our findings provide evidence for the circulation of most HBoV genotypes (except HBoV-4) in the North Region of Brazil at a considerable rate and further investigations are necessary to improve our knowledge in the context of HBoV infections and their role in gastrointestinal diseases.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): acute gastroenteritis , Brazil , children and human bocavirus
© 2019 The Authors
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2019-08-01
2024-04-20
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References

  1. Troeger C, Forouzanfar M, Rao PC, Khalil I, Brown A et al. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the global burden of Disease Study 2015. Lancet Infect Dis 2017; 17:909–948 [View Article]
     [Google Scholar]
  2. Nicholson MR, Van Horn GT, Tang YW, Vinjé J, Payne DC et al. Using multiplex molecular testing to determine the etiology of acute gastroenteritis in children. J Pediatr 2016; 176:50–56 [View Article]
     [Google Scholar]
  3. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA 2005; 102:12891–12896 [View Article]
     [Google Scholar]
  4. Albuquerque MCM, Rocha LN, Benati FJ, Soares CC, Maranhão AG et al. Human bocavirus infection in children with gastroenteritis, Brazil. Emerg Infect Dis 2007; 13:1756–1758 [View Article]
     [Google Scholar]
  5. Guido M, Tumolo MR, Verri T, Romano A, Serio F et al. Human bocavirus: current knowledge and future challenges. World J Gastroenterol 2016; 22:8684–8697 [View Article]
     [Google Scholar]
  6. International Committee on taxonomy of viruses (ICTV). https://talk.ictvonline.org/taxonomy/ 2018 Feb 19
  7. Gurda BL, Parent KN, Bladek H, Sinkovits RS, DiMattia MA et al. Human bocavirus capsid structure: insights into the structural repertoire of the parvoviridae. J Virol 2010; 84:5880–5889 [View Article]
     [Google Scholar]
  8. Cashman O, O’Shea H. Detection of human bocaviruses 1, 2 and 3 in Irish children presenting with gastroenteritis. Arch Virol 2012; 157:1767–1773 [View Article]
     [Google Scholar]
  9. Jartti T, Hedman K, Jartti L, Ruuskanen O, Allander T et al. Human bocavirus-the first 5 years. Rev Med Virol 2012; 22:46–64 [View Article]
     [Google Scholar]
  10. Ong DSY, Schuurman R, Heikens E. Human bocavirus in stool: a true pathogen or an innocent bystander?. J Clin Virol 2016; 74:45–49 [View Article]
     [Google Scholar]
  11. Schildgen O. Human bocavirus: lessons learned to date. Pathogens 2013; 2:1–12 [View Article]
     [Google Scholar]
  12. De R, Liu L, Qian Y, Zhu R, Deng J et al. Risk of acute gastroenteritis associated with human bocavirus infection in children: a systematic review and meta-analysis. PLoS One 2017; 12:e0184833 [View Article]
     [Google Scholar]
  13. Campos GS, Silva Sampaio ML, Menezes ADL, Tigre DM, Moura Costa LF et al. Human bocavirus in acute gastroenteritis in children in Brazil. J Med Virol 2016; 88:166–170 [View Article]
     [Google Scholar]
  14. La Rosa G, Della Libera S, Iaconelli M, Donia D, Cenko F et al. Human bocavirus in children with acute gastroenteritis in Albania. J Med Virol 2016; 88:906–910 [View Article]
     [Google Scholar]
  15. Lasure N, Gopalkrishna V. Molecular epidemiology and clinical severity of human bocavirus (HBoV) 1-4 in children with acute gastroenteritis from Pune, Western India. J Med Virol 2017; 89:17–23 [View Article]
     [Google Scholar]
  16. Alcalá AC, Pérez K, Blanco R, González R, Ludert JE et al. Molecular detection of human enteric viruses circulating among children with acute gastroenteritis in Valencia, Venezuela, before rotavirus vaccine implementation. Gut Pathog 2018; 10:6 [View Article]
     [Google Scholar]
  17. Kim A, Chang JY, Shin S, Yi H, Moon JS et al. Epidemiology and factors related to clinical severity of acute gastroenteritis in hospitalized children after the introduction of rotavirus vaccination. J Korean Med Sci 2017; 32:465–474 [View Article]
     [Google Scholar]
  18. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM et al. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28:495–503
     [Google Scholar]
  19. Kapoor A, Simmonds P, Slikas E, Li L, Bodhidatta L et al. Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis 2010; 201:1633–1643 [View Article]
     [Google Scholar]
  20. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
     [Google Scholar]
  21. Ayres M, Ayres Jr M, Ayres D, Santos A. BIOESTAT –Aplicações estatísticas nas áreas das ciências bio-médicas, 5th ed. Belém,PA: Ong Mamiraua; 2007
     [Google Scholar]
  22. Portes SAR, Carvalho-Costa FA, Rocha MS, Fumian TM, Maranhão AG et al. Enteric viruses in HIV-1 seropositive and HIV-1 seronegative children with diarrheal diseases in Brazil. Plos One 2017; 12:e0183196 [View Article]
     [Google Scholar]
  23. Sousa TT, Souza M, Fiaccadori FS, Borges AMT, Costa PS et al. Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil. Mem Inst Oswaldo Cruz 2012; 107:800–804 [View Article]
     [Google Scholar]
  24. Zhou T, Chen Y, Chen J, Hu P, Zheng T et al. Prevalence and clinical profile of human bocavirus in children with acute gastroenteritis in Chengdu, West China, 2012–2013. J Med Virol 2017; 89:1743–1748 [View Article]
     [Google Scholar]
  25. Alam MM, Khurshid A, Shaukat S, Sharif S, Suleman RM et al. ‘Human bocavirus in Pakistani children with gastroenteritis'. J Med Virol 2015; 87:656–663 [View Article]
     [Google Scholar]
  26. Han TH, Kim CH, Park SH, Kim EJ, Chung JY et al. Detection of human bocavirus-2 in children with acute gastroenteritis in South Korea. Arch Virol 2009; 154:1923–1927 [View Article]
     [Google Scholar]
  27. Arthur JL, Higgins GD, Davidson GP, Givney RC, Ratcliff RM. A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog 2009; 5:e1000391 [View Article]
     [Google Scholar]
  28. Khamrin P, Thongprachum A, Shimizu H, Okitsu S, Mizuguchi M et al. Detection of human bocavirus 1 and 2 from children with acute gastroenteritis in Japan. J Med Virol 2012; 84:901–905 [View Article]
     [Google Scholar]
  29. Levican J, Navas E, Orizola J, Avendaño LF, Gaggero A. Human bocavirus in children with acute gastroenteritis, Chile, 1985–2010. Emerg Infect Dis 2013; 19:1877–1880 [View Article]
     [Google Scholar]
  30. Santos N, Peret TCT, Humphrey CD, Albuquerque MCM, Silva RC et al. Human bocavirus species 2 and 3 in Brazil. J Clin Virol 2010; 48:127–130 [View Article]
     [Google Scholar]
  31. Schildgen O, Müller A, Simon A. Human bocavirus and gastroenteritis. Emerg Infect Dis 2007; 13:1620–1621 [View Article]
     [Google Scholar]
  32. Tymentsev A, Tikunov A, Zhirakovskaia E, Kurilschikov A, Babkin I et al. Human bocavirus in hospitalized children with acute gastroenteritis in Russia from 2010 to 2012. Infect Genet Evol 2016; 37:143–149 [View Article]
     [Google Scholar]
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Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil
J. Med. Microbiol. 68, 1233 (2019); https://doi.org/10.1099/jmm.0.001026
/content/journal/jmm/10.1099/jmm.0.001026
/content/journal/jmm/10.1099/jmm.0.001026
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