@article{mbs:/content/journal/jmm/10.1099/jmm.0.007351-0, author = "Pi, Borui and Yu, Meihong and Chen, Yagang and Yu, Yunsong and Li, Lanjuan", title = "Distribution of the ACME-arcA gene among meticillin-resistant Staphylococcus haemolyticus and identification of a novel ccr allotype in ACME-arcA-positive isolates", journal= "Journal of Medical Microbiology", year = "2009", volume = "58", number = "6", pages = "731-736", doi = "https://doi.org/10.1099/jmm.0.007351-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.007351-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "MRSH, meticillin-resistant Staphylococcus haemolyticus", keywords = "ACME, arginine catabolic mobile element", keywords = "MRSA, meticillin-resistant Staphylococcus aureus", keywords = "CNS, coagulase-negative staphylococci", abstract = "The aim of this study was to investigate the prevalence and characteristics of ACME (arginine catabolic mobile element)-arcA-positive isolates among meticillin-resistant Staphylococcus haemolyticus (MRSH). ACME-arcA, native arcA and SCCmec elements were detected by PCR. Susceptibilities to 10 antimicrobial agents were compared between ACME-arcA-positive and -negative isolates by chi-square test. PFGE was used to investigate the clonal relatedness of ACME-arcA-positive isolates. The phylogenetic relationships of ACME-arcA and native arcA were analysed using the neighbour-joining methods of mega software. A total of 42 (47.7 %) of 88 isolates distributed in 13 PFGE types were positive for the ACME-arcA gene. There were no significant differences in antimicrobial susceptibility between ACME-arcA-positive and -negative isolates. A novel ccr allotype (ccrAB SHP) was identified in ACME-arcA-positive isolates. Among 42 ACME-arcA-positive isolates: 8 isolates harboured SCCmec V, 8 isolates harboured class C1 mec complex and ccrAB SHP; 22 isolates harbouring class C1 mec complex and 4 isolates harbouring class C2 mec complex were negative for all known ccr allotypes. The ACME-arcA-positive isolates were first found in MRSH with high prevalence and clonal diversity, which suggests a mobility of ACME within MRSH. The results from this study revealed that MRSH is likely to be one of the potential reservoirs of ACME for Staphylococcus aureus.", }