1887

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Volume 58, Issue 11

Characterization of multidrug-resistant and extended-spectrum -lactamase-producing strains from Malaysian hospitals Free

Abstract

The emergence of multidrug-resistant (MDR) and extended-spectrum -lactamase (ESBL)-producing poses a serious antibiotic management problem as resistance genes are easily transferred from one organism to another. Fifty-one strains of isolated from sporadic cases in various hospitals throughout Malaysia were analysed by antimicrobial susceptibility testing, PCR detection of ESBL-encoding genes and DNA fingerprinting. Although 27 of the 51 strains were MDR (i.e. resistant to three or more classes of antibiotics), the majority of the strains (98 %) were sensitive to imipenem. PCR detection using ESBL gene-specific primers showed that 46 of the strains harboured , 19 harboured , 5 harboured and 4 harboured . Class 1 integron-encoded integrase was detected in 21 of the 51 strains and amplification of the integron 5′CS region showed the presence of several known antibiotic resistance gene cassettes of various sizes. Results of conjugation and transformation experiments indicated that some of the ESBL-encoding genes (i.e. , and ) were transmissible and were likely plasmid-encoded. DNA fingerprinting using PFGE and PCR-based methods indicated that the 51 strains were genetically diverse and heterogeneous.

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Keyword(s): DDST, double-disc synergy test , ERIC-PCR, enterobacterial repetitive intergenic consensus PCR , ESBL, extended-spectrum β-lactamase , MDR, multidrug resistant , RAPD, random amplification of polymorphic DNA and REP-PCR, repetitive-sequence-based PCR
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2009-11-01
2024-04-19
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Characterization of multidrug-resistant and extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals
J. Med. Microbiol. 58, 1463 (2009); https://doi.org/10.1099/jmm.0.011114-0
/content/journal/jmm/10.1099/jmm.0.011114-0
/content/journal/jmm/10.1099/jmm.0.011114-0
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