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Volume 59, Issue 4

Typing of the pilus-protein-encoding FCT region and biofilm formationas novel parameters in epidemiological investigations of isolates from various infection sites Free

Abstract

is an important human pathogen for whichan association between infection site and selected epidemiological or functionalmarkers has previously been suggested. However, the studies involved oftenused strains with an insufficiently defined clinical background and laboratoryhistory. Thus, the major goal of the present study was to investigate theserelationships in 183 prospectively collected, well-defined, low-passage isolatesfrom a North-East German centre for tertiary care. For each isolate the clinicalbackground (91 respiratory, 71 skin and 21 invasive isolates) andantibiotic-resistance pattern was recorded. All isolates were classified accordingto their type, antibiotic-resistance and PFGE pattern (I restriction analysis of genomic DNA). As novel discriminatorymethods we performed a PCR-based typing of the pilus-protein-encoding FCTregion (FCT) and biofilm-formation phenotyping in various culturemedia. Forty-one isolates were found to be resistant to at least one of thetested antibiotics. typing revealed , , , , and as themost frequent types in our collection. The novel FCT typing showed isolatesencoding FCT types 4 and 2 to be the most common. Overall 113 strains withunique combinations of and FCT types, antibiotic-resistance andPFGE patterns were identified. The majority of all isolates revealed an associationof biofilm-formation capacity with growth media. Comparing all results forpotential associations, no correlation could be established between the anatomicalsite of isolation and the or the FCT type. There was no relationshipbetween biofilm formation and type, antibiotic-resistance orPFGE patterns. However, a novel association between biofilm formation andFCT type became obvious among strains from our collection.

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  • Accepted:
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Keyword(s): CLSI, Clinical and Laboratory Standards Institute and GAS, group A streptococci
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2010-04-01
2024-04-23
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Typing of the pilus-protein-encoding FCT region and biofilm formationas novel parameters in epidemiological investigations of Streptococcuspyogenes isolates from various infection sites
J. Med. Microbiol. 59, 442 (2010); https://doi.org/10.1099/jmm.0.013581-0
/content/journal/jmm/10.1099/jmm.0.013581-0
/content/journal/jmm/10.1099/jmm.0.013581-0
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