Typing of the pilus-protein-encoding FCT region and biofilm formationas novel parameters in epidemiological investigations of Streptococcuspyogenes isolates from various infection sites Köller, Thomas and Manetti, Andrea Guido Oreste and Kreikemeyer, Bernd and Lembke, Cordula and Margarit, Immaculada and Grandi, Guido and Podbielski, Andreas,, 59, 442-452 (2010), doi = https://doi.org/10.1099/jmm.0.013581-0, publicationName = Microbiology Society, issn = 0022-2615, abstract= Streptococcus pyogenes is an important human pathogen for whichan association between infection site and selected epidemiological or functionalmarkers has previously been suggested. However, the studies involved oftenused strains with an insufficiently defined clinical background and laboratoryhistory. Thus, the major goal of the present study was to investigate theserelationships in 183 prospectively collected, well-defined, low-passage isolatesfrom a North-East German centre for tertiary care. For each isolate the clinicalbackground (91 respiratory, 71 skin and 21 invasive isolates) andantibiotic-resistance pattern was recorded. All isolates were classified accordingto their emm type, antibiotic-resistance and PFGE pattern (SmaI restriction analysis of genomic DNA). As novel discriminatorymethods we performed a PCR-based typing of the pilus-protein-encoding FCTregion (FCT) and biofilm-formation phenotyping in various culturemedia. Forty-one isolates were found to be resistant to at least one of thetested antibiotics. emm typing revealed emm28, emm12, emm1, emm4, emm89 and emm2 as themost frequent types in our collection. The novel FCT typing showed isolatesencoding FCT types 4 and 2 to be the most common. Overall 113 strains withunique combinations of emm and FCT types, antibiotic-resistance andPFGE patterns were identified. The majority of all isolates revealed an associationof biofilm-formation capacity with growth media. Comparing all results forpotential associations, no correlation could be established between the anatomicalsite of isolation and the emm or the FCT type. There was no relationshipbetween biofilm formation and emm type, antibiotic-resistance orPFGE patterns. However, a novel association between biofilm formation andFCT type became obvious among strains from our collection., language=, type=