1887

Abstract

A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome (SCC) in meticillin-resistant (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCC types that we tested were correctly characterized by our PCR assay. SCC type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type.

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2010-10-01
2024-03-28
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References

  1. Boye K., Bartels M. D., Andersen I. S., Møller J. A., Westh H. 2007; A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCC mec types I–V. Clin Microbiol Infect 13:725–727 [CrossRef]
    [Google Scholar]
  2. Chongtrakool P., Ito T., Ma X. X., Kondo Y., Trakulsomboon S., Tiensasitorn C., Jamklang M., Chavalit T., Song J. H., Hiramatsu K. 2006; Staphylococcal cassette chromosome mec (SCC mec ) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob Agents Chemother 50:1001–1012 [CrossRef]
    [Google Scholar]
  3. Cookson B. D., Robinson D. A., Monk A. B., Murchan S., Deplano A., de Ryck R., Struelens M. J., Scheel C., Fussing V. other authors 2007; Evaluation of molecular typing methods in characterizing a European collection of epidemic methicillin-resistant Staphylococcus aureus strains: the HARMONY collection. J Clin Microbiol 45:1830–1837 [CrossRef]
    [Google Scholar]
  4. Ghaznavi-Rad E., Shamsudin M. N., Sekawi Z., Khoon L. Y., Aziz M. N., Hamat R. A., Othman N., Chong P. P., van Belkum A. other authors 2010; Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia. J Clin Microbiol 48:867–872 [CrossRef]
    [Google Scholar]
  5. Hartman B. J., Tomasz A. 1984; Low-affinity penicillin-binding protein associated with β -lactam resistance in Staphylococcus aureus . J Bacteriol 158:513–516
    [Google Scholar]
  6. Hsu L. Y., Loomba-Chlebicka N., Koh Y. L., Tan T. Y., Krishnan P., Lin R. T., Tee N. W., Fisher D. A., Koh T. H. 2007; Evolving EMRSA-15 epidemic in Singapore hospitals. J Med Microbiol 56:376–379 [CrossRef]
    [Google Scholar]
  7. Huang Y. C., Su L. H., Wu T. L., Lin T. Y. 2006; Changing molecular epidemiology of methicillin-resistant Staphylococcus aureus bloodstream isolates from a teaching hospital in Northern Taiwan. J Clin Microbiol 44:2268–2270 [CrossRef]
    [Google Scholar]
  8. Ito T., Katayama Y., Hiramatsu K. 1999; Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob Agents Chemother 43:1449–1458
    [Google Scholar]
  9. Ito T., Katayama Y., Asada K., Mori N., Tsutsumimoto K., Tiensasitorn C., Hiramatsu K. 2001; Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus . Antimicrob Agents Chemother 45:1323–1336 [CrossRef]
    [Google Scholar]
  10. Ito T., Okuma K., Ma X. X., Yuzawa H., Hiramatsu K. 2003; Insights on antibiotic resistance of Staphylococcus aureus from its whole genome : genomic island SCC. Drug Resist Updat 6:41–52 [CrossRef]
    [Google Scholar]
  11. Ito T., Ma X. X., Takeuchi F., Okuma K., Yuzawa H., Hiramatsu K. 2004; Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC . Antimicrob Agents Chemother 48:2637–2651 [CrossRef]
    [Google Scholar]
  12. IWG-SCC 2009; Classification of staphylococcal cassette chromosome mec (SCC mec ): guidelines for reporting novel SCC mec elements. Antimicrob Agents Chemother 53:4961–4967 [CrossRef]
    [Google Scholar]
  13. Jevons M. P. 1961; “Celbenin”-resistant staphylococci. BMJ 1:124–125
    [Google Scholar]
  14. Kondo Y., Ito T., Ma X. X., Watanabe S., Kreiswirth B. N., Etienne J., Hiramatsu K. 2007; Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr , and major differences in junkyard regions. Antimicrob Agents Chemother 51:264–274 [CrossRef]
    [Google Scholar]
  15. Lim T. T., Chong F. N., O'Brien F. G., Grubb W. B. 2003; Are all community methicillin-resistant Staphylococcus aureus related? A comparison of their mec regions. Pathology 35:336–343
    [Google Scholar]
  16. Lindsay J. A., Holden M. T. 2004; Staphylococcus aureus : superbug, super genome?. Trends Microbiol 12:378–385 [CrossRef]
    [Google Scholar]
  17. Ma X. X., Galiana A., Pedreira W., Mowszowicz M., Christophersen I., Machiavello S., Lope L., Benaderet S., Buela F. other authors 2005; Community-acquired methicillin-resistant Staphylococcus aureus , Uruguay. Emerg Infect Dis 11:973–976
    [Google Scholar]
  18. Martineau F., Picard F. J., Roy P. H., Ouellette M., Bergeron M. G. 1998; Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus . J Clin Microbiol 36:618–623
    [Google Scholar]
  19. Milheiriço C., Oliveira D. C., de Lencastre H. 2007a; Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus : ‘SCC mec IV multiplex’. J Antimicrob Chemother 60:42–48 [CrossRef]
    [Google Scholar]
  20. Milheiriço C., Oliveira D. C., de Lencastre H. 2007b; Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus . Antimicrob Agents Chemother 51:3374–3377 [CrossRef]
    [Google Scholar]
  21. Ministry of Health 2008 National Surveillance of Antibiotic Resistance Report Malaysia: Ministry of Health; http://www.imr.gov.my/report/Summary%20of%20antibiotic%20resistance%202008.doc
    [Google Scholar]
  22. Nadig S., Ramachandra Raju S., Arakere G. 2010; Epidemic meticillin-resistant Staphylococcus aureus (EMRSA-15) variants detected in healthy and diseased individuals in India. J Med Microbiol 59:815–821 [CrossRef]
    [Google Scholar]
  23. Naimi T. S., LeDell K. H., Como-Sabetti K., Borchardt S. M., Boxrud D. J., Etienne J., Johnson S. K., Vandenesch F., Fridkin S. other authors 2003; Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA 290:2976–2984 [CrossRef]
    [Google Scholar]
  24. Nor Shamsudin M., Sekawi Z., van Belkum A., Neela V. 2008; First community-acquired meticillin-resistant Staphylococcus aureus in Malaysia. J Med Microbiol 57:1180–1181 [CrossRef]
    [Google Scholar]
  25. Okuma K., Iwakawa K., Turnidge J. D., Grubb W. B., Bell J. M., O'Brien F. G., Coombs G. W., Pearman J. W., Tenover F. C. other authors 2002; Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J Clin Microbiol 40:4289–4294 [CrossRef]
    [Google Scholar]
  26. Oliveira D. C., de Lencastre H. 2002; Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus . Antimicrob Agents Chemother 46:2155–2161 [CrossRef]
    [Google Scholar]
  27. Oliveira D. C., Tomasz A., de Lencastre H. 2001; The evolution of pandemic clones of methicillin-resistant Staphylococcus aureus : identification of two ancestral genetic backgrounds and the associated mec elements. Microb Drug Resist 7:349–361 [CrossRef]
    [Google Scholar]
  28. Oliveira D. C., Milheirico C., De Lencastre H. 2006; Redefining a structural variant of staphylococcal cassette chromosome mec , SCC mec type VI. Antimicrob Agents Chemother 50:3457–3459 [CrossRef]
    [Google Scholar]
  29. Pérez-Roth E., Lorenzo-Díaz F., Batista N., Moreno A., Méndez-Alvarez S. 2004; Tracking methicillin-resistant Staphylococcus aureus clones during a 5-year period (1998 to 2002) in a Spanish hospital. J Clin Microbiol 42:4649–4656 [CrossRef]
    [Google Scholar]
  30. Shore A., Rossney A. S., Keane C. T., Enright M. C., Coleman D. C. 2005; Seven novel variants of the staphylococcal chromosomal cassette mec in methicillin-resistant Staphylococcus aureus isolates from Ireland. Antimicrob Agents Chemother 49:2070–2083 [CrossRef]
    [Google Scholar]
  31. Zhang K., McClure J. A., Elsayed S., Louie T., Conly J. M. 2005; Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus . J Clin Microbiol 43:5026–5033 [CrossRef]
    [Google Scholar]
  32. Zhang K., McClure J. A., Elsayed S., Conly J. M. 2009; Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus . Antimicrob Agents Chemother 53:531–540 [CrossRef]
    [Google Scholar]
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