RT Journal Article SR Electronic(1) A1 Ghaznavi-Rad, Ehsanollah A1 Nor Shamsudin, Mariana A1 Sekawi, Zamberi A1 van Belkum, Alex A1 Neela, VasanthakumariYR 2010 T1 A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus JF Journal of Medical Microbiology, VO 59 IS 10 SP 1135 OP 1139 DO https://doi.org/10.1099/jmm.0.021956-0 PB Microbiology Society, SN 1473-5644, AB A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay. SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.021956-0