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Volume 60, Issue 1

Validation of the genital challenge pig model for testing recombinant protein vaccines Free

Abstract

is a Gram-negative obligate intracellular bacterial pathogen that is the leading cause of bacterial sexually transmitted disease in humans in developing countries. A vaccination programme is considered to be the best approach to reduce the prevalence of infections. However, there are still no commercial vaccines. In order to develop effective vaccines, it is important to identify those antigens that elicit a protective immune response, and to develop new and adequate methods and adjuvants for effective vaccine delivery, as conventional methods have failed to induce protective immunity. In order to test different vaccine candidates, animal models are needed. Former studies have used non-primate monkeys, mice or guinea pig infection models. The present study used a pig model for testing recombinant protein vaccines. Two recombinant proteins, polymorphic membrane protein G (PmpG), and secretion and cellular translocation protein C (SctC), were tested for their ability to create protection in a pig challenge model. The vaccines were administered subcutaneously with GNE adjuvant. Six weeks later, animals were challenged intravaginally with serovar E. After a further 4 weeks, the pigs were euthanized. PmpG-immunized pigs were better protected than pigs immunized with the less promising SctC candidate vaccine antigen. Interestingly, significant protection was apparently not correlated with a strong humoral immune response upon subcutaneous immunization. In conclusion, the pig model is useful for studying the efficacy of vaccine candidates against genital human infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): EB, elementary body , HRP, horseradish peroxidase , MOMP, major outer-membrane protein , p.i., post-infection , Pmp, polymorphic membrane protein , RB, reticulate body , SctC, secretion and cellular translocation protein C , T3SS, type 3 secretion system and TCID50, 50 % tissue culture infective dose
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2011-01-01
2024-04-18
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Validation of the Chlamydia trachomatis genital challenge pig model for testing recombinant protein vaccines
J. Med. Microbiol. 60, 117 (2011); https://doi.org/10.1099/jmm.0.024448-0
/content/journal/jmm/10.1099/jmm.0.024448-0
/content/journal/jmm/10.1099/jmm.0.024448-0
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