1887

Abstract

Cardiac infections caused by the foodborne bacterium represent a significant but poorly studied facet of disease. It is not known whether cardiac infections stem solely from host susceptibility, or whether bacterial isolates exist that exhibit a tropism for cardiac tissue. Here we examine the cardio-invasive capacity of a recent cardiac case strain (07PF0776) as well as nine additional outbreak and clinical isolates. Mice infected with the cardiac isolate 07PF0776 had 10-fold more bacteria recovered from heart tissue than those infected with strain 10403S, a well-characterized clinical isolate originally obtained from a human skin lesion. Additional isolates exhibited varied capacities to colonize the hearts of mice; however, those with the highest efficiency of mouse cardiac invasion also demonstrated the highest levels of bacterial invasion in cultured myoblast cells. Our findings strongly suggest that subpopulations of strains have acquired an enhanced ability to target and invade the myocardium.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.027185-0
2011-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/60/4/423.html?itemId=/content/journal/jmm/10.1099/jmm.0.027185-0&mimeType=html&fmt=ahah

References

  1. Adler A., Fimbres A., Marcinak J., Johnson A., Zheng X., Hasegawa S., Shulman S. T. 2009; Inflammatory pseudotumor of the heart caused by Listeria monocytogenes infection. J Infect 58:161–163 [CrossRef]
    [Google Scholar]
  2. Alonzo F. III, Port G. C., Cao M., Freitag N. E. 2009; The posttranslocation chaperone PrsA2 contributes to multiple facets of Listeria monocytogenes pathogenesis. Infect Immun 77:2612–2623 [CrossRef]
    [Google Scholar]
  3. Antolín J., Gutierrez A., Segoviano R., López R., Ciguenza R. 2008; Endocarditis due to Listeria : description of two cases and review of the literature. Eur J Intern Med 19:295–296 [CrossRef]
    [Google Scholar]
  4. Berenguer J., Solera J., Diaz M. D., Moreno S., López-Herce J. A., Bouza E. 1991; Listeriosis in patients infected with human immunodeficiency virus. Rev Infect Dis 13:115–119 [CrossRef]
    [Google Scholar]
  5. Bierne H., Sabet C., Personnic N., Cossart P. 2007; Internalins: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes . Microbes Infect 9:1156–1166 [CrossRef]
    [Google Scholar]
  6. Bishop D. K., Hinrichs D. J. 1987; Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements. J Immunol 139:2005–2009
    [Google Scholar]
  7. Brouqui P., Raoult D. 2001; Endocarditis due to rare and fastidious bacteria. Clin Microbiol Rev 14:177–207 [CrossRef]
    [Google Scholar]
  8. Brusch J. L. 2001; Cardiac infections in the immunosuppressed patient. Infect Dis Clin North Am 15:613–638 xi [CrossRef]
    [Google Scholar]
  9. de Bruijn M. F., van Vianen W., Ploemacher R. E., Bakker-Woudenberg I. A., Campbell P. A., van Ewijk W., Leenen P. J. 1998; Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting. J Immunol Methods 217:27–39 [CrossRef]
    [Google Scholar]
  10. Edman D. C., Pollock M. B., Hall E. R. 1968; Listeria monocytogenes L forms. I. Induction maintenance, and biological characteristics. J Bacteriol 96:352–357
    [Google Scholar]
  11. Graves L. M., Swaminathan B. 2001; PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. Int J Food Microbiol 65:55–62 [CrossRef]
    [Google Scholar]
  12. Graves L. M., Hunter S. B., Ong A. R., Schoonmaker-Bopp D., Hise K., Kornstein L., DeWitt W. E., Hayes P. S., Dunne E. other authors 2005; Microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the United States to contaminated hot dogs and establishment of molecular subtyping-based surveillance for Listeria monocytogenes in the PulseNet network. J Clin Microbiol 43:2350–2355 [CrossRef]
    [Google Scholar]
  13. Haddad F., Berry G., Doyle R. L., Martineau P., Leung T. K., Racine N. 2007; Active bacterial myocarditis: a case report and review of the literature. J Heart Lung Transplant 26:745–749 [CrossRef]
    [Google Scholar]
  14. Hardy J., Francis K. P., DeBoer M., Chu P., Gibbs K., Contag C. H. 2004; Extracellular replication of Listeria monocytogenes in the murine gall bladder. Science 303:851–853 [CrossRef]
    [Google Scholar]
  15. Hardy J., Margolis J. J., Contag C. H. 2006; Induced biliary excretion of Listeria monocytogenes . Infect Immun 74:1819–1827 [CrossRef]
    [Google Scholar]
  16. Hardy J., Chu P., Contag C. H. 2009; Foci of Listeria monocytogenes persist in the bone marrow. Dis Model Mech 2:39–46 [CrossRef]
    [Google Scholar]
  17. Hill E. E., Herijgers P., Herregods M. C., Peetermans W. E. 2006; Evolving trends in infective endocarditis. Clin Microbiol Infect 12:5–12 [CrossRef]
    [Google Scholar]
  18. Hood S., Baxter R. H. 1999; Listeria endocarditis causing aortic root abscess and a fistula to the left atrium. Scott Med J 44:117–118
    [Google Scholar]
  19. Hyland K. V., Asfaw S. H., Olson C. L., Daniels M. D., Engman D. M. 2008; Bioluminescent imaging of Trypanosoma cruzi infection. Int J Parasitol 38:1391–1400 [CrossRef]
    [Google Scholar]
  20. Kales C. P., Holzman R. S. 1990; Listeriosis in patients with HIV infection: clinical manifestations and response to therapy. J Acquir Immune Defic Syndr 3:139–143
    [Google Scholar]
  21. Khan K. M., Pao W., Kendler J. 2001; Epidural abscess and vertebral osteomyelitis caused by Listeria monocytogenes : case report and literature review. Scand J Infect Dis 33:714–716 [CrossRef]
    [Google Scholar]
  22. Lee S. D., Wu C. C., Kuo W. W., Lin J. A., Hwang J. M., Lu M. C., Chen L. M., Hsu H. H., Wang C. K. other authors 2006; Porphyromonas gingivalis -related cardiac cell apoptosis was majorly co-activated by p38 and extracellular signal-regulated kinase pathways. J Periodontal Res 41:39–46 [CrossRef]
    [Google Scholar]
  23. Lindholm A. C. 2008; Prosthetic valve Listeria endocarditis caused septic cerebral embolism. Lakartidningen 105:2670–2671
    [Google Scholar]
  24. Llanwarne N., Badic B., Delugeau V., Landen S. 2007; Spontaneous splenic rupture associated with Listeria endocarditis. Am J Emerg Med 25:1086.e3–1086.e5
    [Google Scholar]
  25. Luo Y., Lee A., Shen H., Radice G. L. 2003; Altering tissue tropism of Listeria monocytogenes by ectopically expressing human E-cadherin in transgenic mice. Microb Pathog 35:57–62 [CrossRef]
    [Google Scholar]
  26. Makaryus A. N., Yang R., Cohen R., Rosman D., Mangion J., Kort S. 2004; A rare case of Listeria monocytogenes presenting as prosthetic valve bacterial endocarditis and aortic root abscess. Echocardiography 21:423–427 [CrossRef]
    [Google Scholar]
  27. McCue M. J., Moore E. E. 1979; Myocarditis with microabscess formation caused by Listeria monocytogenes associated with myocardial infarct. Hum Pathol 10:469–472 [CrossRef]
    [Google Scholar]
  28. Mead P. S., Slutsker L., Dietz V., McCaig L. F., Bresee J. S., Shapiro C., Griffin P. M., Tauxe R. V. 1999; Food-related illness and death in the United States. Emerg Infect Dis 5:607–625 [CrossRef]
    [Google Scholar]
  29. Mueller K. J., Freitag N. E. 2005; Pleiotropic enhancement of bacterial pathogenesis resulting from the constitutive activation of the Listeria monocytogenes regulatory factor PrfA. Infect Immun 73:1917–1926 [CrossRef]
    [Google Scholar]
  30. O'Neil H. S., Marquis H. 2006; Listeria monocytogenes flagella are used for motility, not as adhesins, to increase host cell invasion. Infect Immun 74:6675–6681 [CrossRef]
    [Google Scholar]
  31. Pocar M., Passolunghi D., Moneta A., Donatelli F. 2009; Fulminant prosthetic valve endocarditis caused by Listeria monocytogenes . Eur J Cardiothorac Surg 36:1077 [CrossRef]
    [Google Scholar]
  32. Rua Galisteo O., Keituqwa Yáñez I., López Sánchez L. 2007; Listeria induced ventriculitis. Med Intensiva 31:50–51 [CrossRef]
    [Google Scholar]
  33. Seeliger H. P. R., Höhne K. 1979; Serotyping of Listeria monocytogenes and related species. Methods Microbiol 13:31–49
    [Google Scholar]
  34. Shetron-Rama L. M., Mueller K., Bravo J. M., Bouwer H. G., Way S. S., Freitag N. E. 2003; Isolation of Listeria monocytogenes mutants with high-level in vitro expression of host cytosol-induced gene products. Mol Microbiol 48:1537–1551 [CrossRef]
    [Google Scholar]
  35. Sun A. N., Camilli A., Portnoy D. A. 1990; Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread. Infect Immun 58:3770–3778
    [Google Scholar]
  36. Swaminathan B., Gerner-Smidt P. 2007; The epidemiology of human listeriosis. Microbes Infect 9:1236–1243 [CrossRef]
    [Google Scholar]
  37. Tyler K. M., Luxton G. W., Applewhite D. A., Murphy S. C., Engman D. M. 2005; Responsive microtubule dynamics promote cell invasion by Trypanosoma cruzi . Cell Microbiol 7:1579–1591 [CrossRef]
    [Google Scholar]
  38. Van Stelten A., Simpson J. M., Ward T. J., Nightingale K. K. 2010; Revelation by single-nucleotide polymorphism genotyping that mutations leading to a premature stop codon in inlA are common among Listeria monocytogenes isolates from ready-to-eat foods but not human listeriosis cases. Appl Environ Microbiol 76:2783–2790 [CrossRef]
    [Google Scholar]
  39. Vázquez-Boland J. A., Kuhn M., Berche P., Chakraborty T., Domínguez-Bernal G., Goebel W., González-Zorn B., Wehland J., Kreft J. 2001; Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 14:584–640 [CrossRef]
    [Google Scholar]
  40. Weaver R. 1989; Morphological, physiological and biochemical characterization. In Isolation of and Identification of Listeria monocytogenes , 1st edn. pp 39–43 Edited by Jones G. L. CDC Laboratory Manual;
    [Google Scholar]
  41. Wollert T., Pasche B., Rochon M., Deppenmeier S., van den Heuvel J., Gruber A. D., Heinz D. W., Lengeling A., Schubert W. D. 2007; Extending the host range of Listeria monocytogenes by rational protein design. Cell 129:891–902 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.027185-0
Loading
/content/journal/jmm/10.1099/jmm.0.027185-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error