@article{mbs:/content/journal/jmm/10.1099/jmm.0.029868-0, author = "Khosravi, Yalda and Tay, Sun Tee and Vadivelu, Jamuna", title = "Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia", journal= "Journal of Medical Microbiology", year = "2011", volume = "60", number = "7", pages = "988-994", doi = "https://doi.org/10.1099/jmm.0.029868-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.029868-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "In this study, 90 non-replicate imipenem-resistant Pseudomonas aeruginosa (IRPA) Malaysian isolates collected between October 2005 and March 2008 were subjected to a screening test for detection of the integron and the gene cassette. Class 1 integrons were detected in 54 IRPA clinical isolates, whilst three isolates contained class 2 integrons. Analysis of the gene cassettes associated with the class 1 integrons showed the detection of accC1 in isolates carrying bla IMP-7 and aacA7 in isolates carrying bla VIM-2. aadA6 was detected in two isolates carrying bla IMP-4. Using random amplification of polymorphic DNA analysis, 14 PCR fingerprint patterns were generated from the 32 isolates carrying metallo-β-lactamase (MBL) genes (35.5 %), whilst 20 patterns were generated from the 58 non-MBL gene isolates (64.4 %). Based on the differences in the fingerprinting patterns, two clusters (A and B) were identified among the MBL-producing isolates. Cluster A comprised 18 isolates (56 %) carrying the bla VIM gene, whereas cluster B comprised 14 (44 %) isolates carrying the bla IMP gene. The non-MBL isolates were divided into clusters C and D. Cluster C comprised 22 non-MBL isolates harbouring class 1 integrons, whilst cluster D consisted of three isolates carrying class 2 integrons. These findings suggest that the class 1 integron is widespread among P. aeruginosa isolated in Malaysia and that characterization of cassette arrays of integrons will be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes.", }