1887

Journal of Medical Microbiology logo

Volume 52, Issue 4

Involvement of nitric oxide donor compounds in the bactericidal activity of human neutrophils Free

Abstract

The bactericidal activity of human neutrophils against extracellular and facultatively intracellular bacteria was studied in the presence of the nitric oxide (NO) donors sodium nitroprusside (SNP) and 3-morpholinosydnonimine (SIN-1), a molsidomine metabolite. SNP and molsidomine are drugs commonly used as nitrovasodilators in coronary heart disease. It is demonstrated here that the NO donor compounds themselves did not affect the viability and survival of the bacterial strains tested. Neither SNP nor SIN-1 had any effect on the process of bacteria ingestion. In contrast, NO donors enhanced the ability of neutrophils to kill , and Anatum. However, strains differed in their susceptibility to SNP- and SIN-1-mediated killing by neutrophils. Removal of the superoxide anion reduced the bactericidal activity of SNP- and SIN-1-treated neutrophils against and . Anatum. This suggests that the NO derivatives formed in the reaction of NO generated from donors with the reactive oxygen species released by phagocytosed neutrophils potentiate the bactericidal activity of human neutrophils . The above original observation discussed here suggests clinical significance for the treatment of patients with nitrovasodilators in the course of coronary heart disease therapy.

  • Published Online:
© 2003 Society for General Microbiology
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.04974-0
2003-04-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/52/4/303.html?itemId=/content/journal/jmm/10.1099/jmm.0.04974-0&mimeType=html&fmt=ahah

References

  1. Armstrong R. 2001; The physiological role and pharmacological potential of nitric oxide in neutrophil activation. Int Immunopharmacol 1:1501–1512 [CrossRef]
     [Google Scholar]
  2. Babior B. M. 1999; NADPH oxidase: an update. Blood 93:1464–1476
     [Google Scholar]
  3. Bogdan C, Röllinghoff M, Diefenbach A. 2000; Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity. Curr Opin Immunol 12:64–76 [CrossRef]
     [Google Scholar]
  4. Brunelli L, Crow J. P, Beckman J. S. 1995; The comparative toxicity of nitric oxide and peroxynitrite to Escherichia coli . Arch Biochem Biophys 316:327–334 [CrossRef]
     [Google Scholar]
  5. Chmiela M, Wadstrom T, Folkesson H, Planeta Malecka I, Czkwianianc E, Rechcinski T, Rudnicka W. 1998; Anti-Lewis X antibody and Lewis X–anti-Lewis X immune complexes in Helicobacter pylori infection. Immunol Lett 61:119–125 [CrossRef]
     [Google Scholar]
  6. Clancy R. M, Amin A. R, Abramson S. B. 1998; The role of nitric oxide in inflammation and immunity. Arthritis Rheum 41:1141–1151 [CrossRef]
     [Google Scholar]
  7. Cochran J. B, Genovese F, Ogura S, Teti G, Cook J. A. 1999; Effect of nitric oxide donors and nitric oxide synthase inhibitors in neonatal rat endotoxic shock. Biochem Pharmacol 58:687–691 [CrossRef]
     [Google Scholar]
  8. Dahlgren C, Karlsson A. 1999; Respiratory burst in human neutrophils. J Immunol Methods 232:3–14 [CrossRef]
     [Google Scholar]
  9. De Groote M. A, Fang F. C. 1995; NO inhibitions: antimicrobial properties of nitric oxide. Clin Infect Dis 21:Suppl. 2S162–S165
     [Google Scholar]
  10. De Groote M. A, Granger D, Xu Y, Campbell G, Prince R, Fang F. C. 1995; Genetic and redox determinants of nitric oxide cytotoxicity in a Salmonella typhimurium model. Proc Natl Acad Sci U S A 92:6399–6403
     [Google Scholar]
  11. Doulias P. T, Barbouti A, Galaris D, Ischiropoulos H. 2001; SIN-1-induced DNA damage in isolated human peripheral blood lymphocytes as assessed by single cell gel electrophoresis (comet assay). Free Radic Biol Med 30:679–685 [CrossRef]
     [Google Scholar]
  12. Eiserich J. P, Hristova M, Cross C. E, Jones A. D, Freeman B. A, Halliwell B, Van der Vliet A. 1998; Formation of nitric oxide-derived inflammatory oxidants by myeloperoxidase in neutrophils. Nature 391:393–397 [CrossRef]
     [Google Scholar]
  13. Fang F. C. 1997; Mechanisms of nitric oxide-related antimicrobial activity. J Clin Invest 99:2818–2825 [CrossRef]
     [Google Scholar]
  14. Feelisch M. J. 1991; The biochemical pathways of nitric oxide formation from nitrovasodilators: appropriate choice of exogenous NO donors and aspects of preparation and handling of aqueous solutions. J Cardiovasc Pharmacol 17:Suppl. 3S25–S33
     [Google Scholar]
  15. Fierro I. M, Barja-Fidalgo C, Cunha F. Q, Ferreira S. H. 1996; The involvement of nitric oxide in the anti- Candida albicans activity of rat neutrophils. Immunology 89:295–300 [CrossRef]
     [Google Scholar]
  16. Forslund T, Sundqvist T. 1995a; Nitric oxide regulates the chemiluminescence from stimulated human neutrophils. APMIS 103:813–817 [CrossRef]
     [Google Scholar]
  17. Forslund T, Sundqvist T. 1995b; Nitric oxide reduces hydrogen peroxide production from human polymorphonuclear neutrophils. Eur J Clin Invest 25:9–14 [CrossRef]
     [Google Scholar]
  18. Forslund T, Sundqvist T. 1997; Nitric oxide-releasing particles inhibit phagocytosis in human neutrophils. Biochem Biophys Res Commun 233:492–495 [CrossRef]
     [Google Scholar]
  19. Green L. C, Wagner D. A, Glogowski J, Skipper P. L, Wishnok J. S, Tannenbaum S. R. 1982; Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 126:131–138 [CrossRef]
     [Google Scholar]
  20. Janero D. R. 2000; Nitric oxide (NO)-related pharmaceuticals: contemporary approaches to therapeutic NO modulation. Free Radic Biol Med 28:1495–1506 [CrossRef]
     [Google Scholar]
  21. Jones R. D, Hancock J. T, Morice A. H. 2000; NADPH oxidase: a universal oxygen sensor?. Free Radic Biol Med 29:416–424 [CrossRef]
     [Google Scholar]
  22. Kumins N. H, Hunt J, Gamelli R. L, Filkins J. P. 1997; Molsidomine increases endotoxic survival and decreases cytokine production. Shock 7:200–205 [CrossRef]
     [Google Scholar]
  23. Larfars G, Gyllenhammar H. 1998; Stimulus-dependent transduction mechanisms for nitric oxide release in human polymorphonuclear neutrophil leukocytes. J Lab Clin Med 132:54–60 [CrossRef]
     [Google Scholar]
  24. Malawista S. E, Montgomery R. R, van Blaricom G. 1992; Evidence for reactive nitrogen intermediates in killing of staphylococci by human neutrophil cytoplasts. A new microbicidal pathway for polymorphonuclear leukocytes. J Clin Invest 90:631–636
     [Google Scholar]
  25. Miller R. A, Britigan B. E. 1997; Role of oxidants in microbial pathophysiology. Clin Microbiol Rev 10:1–18
     [Google Scholar]
  26. Pacelli R, Wink D. A, Cook J. A, Krishna M. C, DeGraff W, Friedman N, Tsokos M, Samuni A, Mitchell J. B. 1995; Nitric oxide potentiates hydrogen peroxide-induced killing of Escherichia coli . J Exp Med 182:1469–1479 [CrossRef]
     [Google Scholar]
  27. Saran M, Beck-Speier I, Fellerhoff B, Bauer G. 1999; Phagocytic killing of microorganisms by radical processes: consequences of the reaction of hydroxyl radicals with chloride yielding chlorine atoms. Free Radic Biol Med 26:482–490 [CrossRef]
     [Google Scholar]
  28. Squadrito G. L, Pryor W. A. 1998; Oxidative chemistry of nitric oxide: the role of superoxide, peroxynitrite, and carbon dioxide. Free Radic Biol Med 25:392–403 [CrossRef]
     [Google Scholar]
  29. Stevens M. G, Olsen S. C. 1993; Comparative analysis of using MTT and XTT in colorimetric assays for quantitating bovine neutrophil bactericidal activity. J Immunol Methods 157:225–231 [CrossRef]
     [Google Scholar]
  30. Umeki S. 1994; Activation factors of neutrophil NADPH oxidase complex. Life Sci 55:1–13
     [Google Scholar]
  31. Verhoef J, Visser M. R. 1993; Neutrophil phagocytosis and killing: normal function and microbial evasion. In The Neutrophils pp 109–137 Edited by Abramson J. S., Wheeler J. G. Oxford, New York & Tokyo: IRL Press;
     [Google Scholar]
  32. Yamamoto T, Bing R. J. 2000; Nitric oxide donors. Proc Soc Exp Biol Med 225:200–206 [CrossRef]
     [Google Scholar]
  33. Yan L, Vandivier R. W, Suffredini A. F, Danner R. L. 1994; Human polymorphonuclear leukocytes lack detectable nitric oxide synthase activity. J Immunol 153:1825–1834
     [Google Scholar]
  34. Zhu L, Gunn C, Beckman J. S. 1992; Bactericidal activity of peroxynitrite. Arch Biochem Biophys 298:452–457 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.04974-0
Loading
Involvement of nitric oxide donor compounds in the bactericidal activity of human neutrophils in vitro
J. Med. Microbiol. 52, 303 (2003); https://doi.org/10.1099/jmm.0.04974-0
/content/journal/jmm/10.1099/jmm.0.04974-0
/content/journal/jmm/10.1099/jmm.0.04974-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error