RT Journal Article SR Electronic(1) A1 Ulrich, Ricky L A1 DeShazer, David A1 Brueggemann, Ernst E A1 Hines, Harry B A1 Oyston, Petra C A1 Jeddeloh, Jeffrey AYR 2004 T1 Role of quorum sensing in the pathogenicity of Burkholderia pseudomallei JF Journal of Medical Microbiology, VO 53 IS 11 SP 1053 OP 1064 DO https://doi.org/10.1099/jmm.0.45661-0 PB Microbiology Society, SN 1473-5644, AB Burkholderia pseudomallei is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the in vivo pathogenicity of B. pseudomallei via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that B. pseudomallei encodes a minimum of three luxI and five luxR homologues that are involved in animal pathogenicity. Mass spectrometry analysis of culture supernatants revealed that wild-type B. pseudomallei and the luxI mutants synthesized numerous signalling molecules, including N-octanoyl-homoserine lactone, N-decanoyl-homoserine lactone, N-(3-hydroxyoctanoyl)-l-homoserine lactone, N-(3-hydroxydecanoyl)-l-homoserine lactone and N-(3-oxotetradecanoyl)-l-homoserine lactone, which was further confirmed by heterologous expression of the B. pseudomallei luxI alleles in Escherichia coli. Mutagenesis of the B. pseudomallei QS system increased the time to death and reduced organ colonization of aerosolized BALB/c mice. Further, intraperitoneal challenge of Syrian hamsters with the B. pseudomallei QS mutants resulted in a significant increase in the LD50. Using semi-quantitative plate assays, preliminary analysis suggests that QS does not affect lipase, protease and phospholipase C biosynthesis/secretion in B. pseudomallei. The findings of the investigation demonstrate that B. pseudomallei encodes multiple luxIR genes, and disruption of the QS alleles reduces animal pathogenicity, but does not affect exoproduct secretion., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45661-0