@article{mbs:/content/journal/jmm/10.1099/jmm.0.45773-0, author = "van Dissel, Jaap T and de Groot, Nanda and Hensgens, Charles MH and Numan, Sandra and Kuijper, Ed J and Veldkamp, Peter and van 't Wout, Jan", title = "Bovine antibody-enriched whey to aid in the prevention of a relapse of Clostridium difficile-associated diarrhoea: preclinical and preliminary clinical data", journal= "Journal of Medical Microbiology", year = "2005", volume = "54", number = "2", pages = "197-205", doi = "https://doi.org/10.1099/jmm.0.45773-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45773-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "In a pilot study, the feasibility of immune whey protein concentrate (40 %; immune WPC-40) to aid the prevention of relapse of Clostridium difficile diarrhoea was evaluated. Immune WPC-40 was made from milk after immunization of Holstein–Frisian cows with C. difficile-inactivated toxins and killed whole-cell C. difficile. Immune WPC-40 contained a high concentration of specific sIgA antibodies, and was effective in neutralizing the cytotoxic effect of C. difficile toxins in cell assays in vitro. Immune WPC-40 conferred protection from otherwise lethal C. difficile-associated caecitis in hamsters. To obtain preliminary data in humans, 16 patients (10 male; median 57 years) with toxin- and culture-confirmed C. difficile diarrhoea were enrolled in an uncontrolled cohort study. Nine had a history of relapsing C. difficile diarrhoea. After completion of standard antibiotic treatment, the patients received immune WPC-40 TID for 2 weeks; it was well tolerated and no treatment-related adverse effects were observed. In all but one case, C. difficile toxins had disappeared from the faeces upon completion of treatment. During a follow-up period of median 333 days (range 35 days to 1 year), none of the patients had suffered another episode of C. difficile diarrhoea. These preliminary data suggest that immune whey protein concentrate-40 may be of help in the prevention of relapse of C. difficile diarrhoea.", }