An improved protocol for pulsed-field gel electrophoresis typing of Clostridium difficile
Alonso, R and Martín, A and Peláez, T and Marín, M and Rodríguez-Creixéms, M and Bouza, E,, 54, 155-157 (2005),
doi = https://doi.org/10.1099/jmm.0.45808-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= Pulsed-field gel electrophoresis (PFGE) is the ‘gold standard’ technique for bacterial typing and has proved to be discriminatory and reproducible for typing Clostridium difficile. Nevertheless, a high proportion of strains are non-typable by this technique due to the degradation of the DNA during the process. The introduction of several modifications in the PFGE standard procedure increased typability from 40 % (90 isolates) to 100 % (220 isolates) while maintaining the high degree of discrimination and reproducibility of the technique.,
language=,
type=