1887

Abstract

The aims of this study were to identify hydrogen sulfide (HS)-producing bacteria among tongue biofilm microflora and to investigate the relationship between bacterial flora and HS levels in mouth air. Oral malodour levels in 10 subjects (age 21–56 years) were assessed by gas chromatography, and Breathtron and organoleptic scores. Based on these assessments, subjects were divided into two groups: an odour group and a no/low odour group. Tongue coatings were sampled and spread onto Fastidious Anaerobe Agar plates containing 0.05 % cysteine, 0.12 % glutathione and 0.02 % lead acetate, and were then incubated anaerobically at 37 °C for 2 weeks. Bacteria forming black or grey colonies were selected as HS-producing phenotypes. The numbers of total bacteria ( < 0.005) and HS-producing bacteria ( < 0.05) in the odour group were significantly larger than those in the no/low odour group. Bacteria forming black or grey colonies (126 isolates from the odour group; 242 isolates from the no/low odour group) were subcultured, confirmed as producing HS and identified according to 16S rRNA gene sequencing. Species of (38.1 % in odour group; 46.3 % in no/low odour group), (25.4 %; 17.7 %) and (10.3 %; 7.8 %) were the predominant HS-producing bacteria in both the odour and no/low odour groups. These results suggest that an increase in the number of HS-producing bacteria in the tongue biofilm is responsible for oral malodour, although the bacterial composition of tongue biofilm was similar between the two groups.

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2005-09-01
2024-03-28
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References

  1. Awano S., Gohara K., Kurihara E., Ansai T., Takehara T. 2002; The relationship between the presence of periodontopathogenic bacteria in saliva and halitosis. Int Dent J 52:212–216 [CrossRef]
    [Google Scholar]
  2. Ayers K. M., Colquhoun A. N. 1998; Halitosis: causes, diagnosis, and treatment. N Z Dent J 94:156–160
    [Google Scholar]
  3. Claesson R., Edlund M. B., Persson S., Carlsson J. 1990; Production of volatile sulfur compounds by various Fusobacterium species. Oral Microbiol Immunol 5:137–142 [CrossRef]
    [Google Scholar]
  4. De Boever E. H., Loesche W. J. 1995; Assessing the contribution of anaerobic microflora of the tongue to oral malodor. J Am Dent Assoc 126:1384–1393 [CrossRef]
    [Google Scholar]
  5. Distler W., Kröncke A. 1981; Acid formation by mixed cultures of dental plaque bacteria Actinomyces and Veillonella . Arch Oral Biol 26:123–126 [CrossRef]
    [Google Scholar]
  6. Donaldson A. C., McKenzie D., Riggio M. P., Hodge P. J., Rolph H., Flanagan A., Bagg J. 2005; Microbiological culture analysis of the tongue anaerobic microflora in subjects with and without halitosis. Oral Dis 11 (Suppl. 1):61–63
    [Google Scholar]
  7. Frisken K. W., Higgins T., Palmer J. M. 1990; The incidence of periodontopathic microorganisms in young children. Oral Microbiol Immunol 5:43–45 [CrossRef]
    [Google Scholar]
  8. Gordon D. F. Jr, Gibbons R. J. 1966; Studies of the predominant cultivable microorganisms from the human tongue. Arch Oral Biol 11:627–632 [CrossRef]
    [Google Scholar]
  9. Greenman J. 1999; Microbial aetiology of halitosis. In Dental Plaque Revisited. Oral Biofilms in Health and Disease pp 419–442 Edited by Newman H. N, Wilson M. Cardiff: BioLine;
    [Google Scholar]
  10. Hartley M. G., El-Maaytah M. A., McKenzie C., Greenman J. 1996; The tongue microbiota of low odour and malodorous individuals. Microb Ecol Health Dis 9:215–223 [CrossRef]
    [Google Scholar]
  11. Hartley G., McKenzie C., Greenman J., El-Maaytah M. A., Scully C., Porter S. 1999; Tongue microbiota and malodour.Effects of metronidazole mouthrinse on tongue microbiota and breath odour. Microb Ecol Health Dis 11:226–233
    [Google Scholar]
  12. Kazor C. E., Mitchell P. M., Lee A. M., Stokes L. N., Loesche W. J., Dewhirst F. E., Paster B. J. 2003; Diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients. J Clin Microbiol 41:558–563 [CrossRef]
    [Google Scholar]
  13. Kleinberg I., Westbay G. 1990; Oral malodor. Crit Rev Oral Biol Med 1:247–259
    [Google Scholar]
  14. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E, Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  15. Loesche W. J., Kazor C. 2002; Microbiology and treatment of halitosis. Periodontol 2000; 28:256–279 [CrossRef]
    [Google Scholar]
  16. Mager D. L., Ximenez-Fyvie L. A., Haffajee A. D., Socransky S. S. 2003; Distribution of selected bacterial species on intraoral surfaces. J Clin Periodontol 30:644–654 [CrossRef]
    [Google Scholar]
  17. Milnes A. R., Bowden G. H., Gates D., Tate R. 1993; Predominant cultivable microorganisms on the tongue of preschool children. Microb Ecol Health Dis 6:229–235 [CrossRef]
    [Google Scholar]
  18. Miyake Y., Iwai T., Sugai M., Miura M., Suginaka H., Nagasaka N. 1991; Incidence and characterization of Staphylococcus aureus from the tongues of children. J Dent Res 70:1045–1047 [CrossRef]
    [Google Scholar]
  19. Miyazaki H., Sakao S., Katoh Y., Takehara T. 1995; Correlation between volatile sulphur compounds and certain oral health measurements in the general population. J Periodontol 66:679–684 [CrossRef]
    [Google Scholar]
  20. Nakano Y., Yoshimura M., Koga T. 2002; Correlation between oral malodor and periodontal bacteria. Microbes Infect 4:679–683 [CrossRef]
    [Google Scholar]
  21. Nara F. 1977; The relationship between the halitosis and oral conditions of the periodontal patients. J Jpn Assoc Periodontol 19:100–108 [CrossRef]
    [Google Scholar]
  22. O'Leary T. J., Drake R. B., Naylor J. E. 1972; The plaque control record. J Periodontol 43:38–39 [CrossRef]
    [Google Scholar]
  23. Paryavi-Gholami F., Minah G. E., Turng B. F. 1999; Oral malodor in children and volatile sulfur compound-producing bacteria in saliva: preliminary microbiological investigation. Pediatr Dent 21:320–324
    [Google Scholar]
  24. Persson S., Edlund M. B., Claesson R., Carlsson J. 1990; The formation of hydrogen sulfide and methyl mercaptan by oral bacteria. Oral Microbiol Immunol 5:195–201 [CrossRef]
    [Google Scholar]
  25. Pianotti R., Lachette S., Dills S. 1986; Desulfuration of cysteine and methionine by Fusobacterium nucleatum . J Dent Res 65:913–917 [CrossRef]
    [Google Scholar]
  26. Roldan S., Herrera D., Sanz M. 2003; Biofilms and the tongue: therapeutical approaches for the control of halitosis. Clin Oral Investig 7:189–197 [CrossRef]
    [Google Scholar]
  27. Rosenberg M. 1996; Clinical assessment of bad breath: current concepts. J Am Dent Assoc 127:475–482 [CrossRef]
    [Google Scholar]
  28. Schaal K. P. 1986; Genus Actinomyces . In Bergey's Manual of Systematic Bacteriology vol 2 pp 1383–1418 Edited by Sneath P. H. A, Mair N. S, Sharpe M. E, Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  29. Scully C., Porter S., Greenman J. 1994; What to do about halitosis. BMJ 308:217–218 [CrossRef]
    [Google Scholar]
  30. Shah H. N., Collins D. M. 1990; Prevotella , a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides . Int J Syst Bacteriol 40:205–208 [CrossRef]
    [Google Scholar]
  31. Shibuya K. 2001; Constituents and origins of physiological malodor. J Dent Health 51:778–792
    [Google Scholar]
  32. Shimura M., Yasuno Y., Iwakura M., Shimada Y., Sakai S., Suzuki K., Sakamoto S. 1996; A new monitor with a zinc-oxide thin film semiconductor sensor for the measurement of volatile sulfur compounds in mouth air. J Periodontol 67:396–402 [CrossRef]
    [Google Scholar]
  33. van Winkelhoff A. J., van der Velden U., Winkel E. G., de Graaff J. 1986; Black-pigmenting Bacteroides and motile organisms on oral mucosal surfaces in individuals with and without periodontal breakdown. J Periodontal Res 21:434–439 [CrossRef]
    [Google Scholar]
  34. Yaegaki K., Sanada K. 1992a; Biochemical and clinical factors influencing oral malodor in periodontal patients. J Periodontol 63:783–789 [CrossRef]
    [Google Scholar]
  35. Yaegaki K., Sanada K. 1992b; Volatile sulfur compounds in mouth air from clinically healthy subjects and patients with periodontal disease. J Periodontal Res 27:233–238 [CrossRef]
    [Google Scholar]
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