1887

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Volume 55, Issue 6

Identification of complex pathogens by rapid-cycle PCR with fluorescent hybridization probes Free

Abstract

Members of the complex are important bacterial pathogens in cystic fibrosis (CF) patients. The complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. In this study, a new approach was developed for the rapid identification of genomovar I, (genomovar II), (lineage III-A and III-B), (genomovar IV) and (genomovar V), which cause the large majority of infections in CF patients. The method was based on the detection of differences in the gene sequence by using rapid-cycle PCR and genomovar-specific fluorescence resonance energy transfer (FRET) probes. The genomovar status of all 39 complex strains tested (genomovars I–V) was identified by melting-curve analysis. Each FRET probe produced a specific fluorescence signal only with the respective genomovar, and not with other complex strains and spp. The identification system was easy to handle and revealed complex genomovar I–V status from culture isolates within about 1 h.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CF, cystic fibrosis and FRET, fluorescence resonance energy transfer
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2006-06-01
2024-04-25
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Identification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes
J. Med. Microbiol. 55, 721 (2006); https://doi.org/10.1099/jmm.0.46457-0
/content/journal/jmm/10.1099/jmm.0.46457-0
/content/journal/jmm/10.1099/jmm.0.46457-0
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