1887
  1. Home
  2. Publications
  3. Journal of Medical Microbiology
  4. Volume 55, Issue 11
  5. Article

f An outbreak of psittacosis due to genotype A in a veterinary teaching hospital

HTML
49.29 Kb
PDF
99.43 Kb
  • Authors: Edou R. Heddema1,7 ,  Erik J. van Hannen2 ,  Birgitta Duim1 ,  Bartelt M. de Jongh2 ,  Jan A. Kaan3 ,  Rob van Kessel4 ,  Johannes T. Lumeij5 ,  Caroline E. Visser1 , Christina M. J. E. Vandenbroucke-Grauls1,6
    • VIEW AFFILIATIONS
    • 1 1Department of Medical Microbiology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands 2 2Department of Medical Microbiology and Immunology, St Antonius Hospital, Nieuwegein, The Netherlands 3 3Department of Medical Microbiology and Immunology, Diakonessen Hospital, Utrecht, The Netherlands 4 4Department of Infectious Diseases and Hygiene, Municipal Health Service, Utrecht, The Netherlands 5 5Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Division of Avian and Exotic Animal Medicine, University of Utrecht, Utrecht, The Netherlands 6 6Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, The Netherlands
  • Edou R. Heddema
 [email protected]
  • First Published Online: 01 November 2006, Journal of Medical Microbiology 55: 1571-1575, doi: 10.1099/jmm.0.46692-0
  • Subject: Clinical Microbiology And Virology
  • Received:
  • Accepted:
  • Cover date:
Preview this:
Free Preview thumbnail Magnify
Zoom in
Zoomout

An outbreak of psittacosis due to genotype A in a veterinary teaching hospital, Page 1 of 1

| /docserver/preview/fulltext/jmm/55/11/1571-1.gif

  • An outbreak of psittacosis in a veterinary teaching hospital was recognized in December 2004. Outbreak management was instituted to evaluate the extent of the outbreak and to determine the avian source. Real-time PCR, serologic testing and sequencing of the gene of were performed. Sputum samples from patients, throat-swab samples from exposed students and staff, and faecal specimens from parrots and pigeons were tested. In this outbreak, 34 % (10/29) of the tested individuals were infected. The clinical features of the infection ranged from none to sepsis with multi-organ failure requiring intensive-care-unit admission. genotype A was identified as the outbreak strain. Parrots, recently exposed to a group of cockatiels coming from outside the teaching facility, which were used in a practical class, appeared to be the source of the outbreak. One of the tested pigeons harboured an unrelated genotype B strain. The microbiological diagnosis by real-time PCR on clinical specimens allowed for rapid outbreak management; subsequent genotyping of the isolates identified the avian source. Recommendations are made to reduce the incidence and extent of future outbreaks.

  • The GenBank/EMBL/DDBJ accession numbers for the sequences of the gene of the OSV outbreak strain and the unrelated CLV pigeon isolate are DQ230095 and DQ230096, respectively.

  • † VU University Medical Centre, Department of Medical Microbiology and Infection Control, PO Box 7057, 1007 MB Amsterdam, The Netherlands.

SGM | Published by the Microbiology Society

  • Boom, R., Sol, C. J., Salimans, M. M., Jansen, C. L., Wertheim-Van Dillen, P. M. & van der Noordaa, J. ( 1990; ). Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28, 495–503.
     [Google Scholar]
  • Boom, R., Sol, C., Weel, J., Lettinga, K., Gerrits, Y., van Breda, A. & Wertheim-van Dillen, P. ( 2000; ). Detection and quantitation of human cytomegalovirus DNA in faeces. J Virol Methods 84, 1–14.[CrossRef]
    [Google Scholar]
  • Bourke, S. J., Carrington, D., Frew, C. E., Stevenson, R. D. & Banham, S. W. ( 1989; ). Serological cross-reactivity among chlamydial strains in a family outbreak of psittacosis. J Infect 19, 41–45.[CrossRef]
    [Google Scholar]
  • Bush, R. M. & Everett, K. D. ( 2001; ). Molecular evolution of the Chlamydiaceae. Int J Syst Evol Microbiol 51, 203–220.
     [Google Scholar]
  • Geens, T., Desplanques, A., Van Loock, M., Bonner, B. M., Kaleta, E. F., Magnino, S., Andersen, A. A., Everett, K. D. & Vanrompay, D. ( 2005; ). Sequencing of the Chlamydophila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method. J Clin Microbiol 43, 2456–2461.[CrossRef]
    [Google Scholar]
  • Heddema, E. R., Beld, M., de Wever, B., Langerak, A. A. J., Pannekoek, Y. & Duim, B. ( 2006a; ). Development of an internally controlled real-time PCR assay for detection of Chlamydophila psittaci in the LightCycler 2.0 system. Clin Microbiol Infect 12, 571–575.[CrossRef]
    [Google Scholar]
  • Heddema, E. R., ter Sluis, S., Buijs, J. A., Vandenbroucke-Grauls, C. M. J. E., Van Wijnen, J. H. & Visser, C. E. ( 2006b; ). Prevalence of Chlamydophila psittaci in fecal droppings from feral pigeons in Amsterdam, The Netherlands. Appl Environ Microbiol 72, 4423–4425.[CrossRef]
    [Google Scholar]
  • Huminer, D., Samra, Z., Weisman, Y. & Pitlik, S. ( 1988; ). Family outbreaks of psittacosis in Israel. Lancet 2, 615–618.
     [Google Scholar]
  • Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  • Menendez, R., Cordoba, J., de la Cuadra, P., Cremades, M. J., Lopez-Hontagas, J. L., Salavert, M. & Gobernado, M. ( 1999; ). Value of the polymerase chain reaction assay in noninvasive respiratory samples for diagnosis of community-acquired pneumonia. Am J Respir Crit Care Med 159, 1868–1873.[CrossRef]
    [Google Scholar]
  • Persson, K. & Haidl, S. ( 2000; ). Evaluation of a commercial test for antibodies to the chlamydial lipopolysaccharide (Medac) for serodiagnosis of acute infections by Chlamydia pneumoniae (TWAR) and Chlamydia psittaci. APMIS 108, 131–138.[CrossRef]
    [Google Scholar]
  • Ramirez, J. A., Ahkee, S., Tolentino, A., Miller, R. D. & Summersgill, J. T. ( 1996; ). Diagnosis of Legionella pneumophila, Mycoplasma pneumoniae, or Chlamydia pneumoniae lower respiratory infection using the polymerase chain reaction on a single throat swab specimen. Diagn Microbiol Infect Dis 24, 7–14.[CrossRef]
    [Google Scholar]
  • Schlossberg, D., Delgado, J., Moore, M. M., Wishner, A. & Mohn, J. ( 1993; ). An epidemic of avian and human psittacosis. Arch Intern Med 153, 2594–2596.[CrossRef]
    [Google Scholar]
  • Schneeberger, P. M., Dorigo-Zetsma, J. W., van der Zee, A., van Bon, M. & van Opstal, J. L. ( 2004; ). Diagnosis of atypical pathogens in patients hospitalized with community-acquired respiratory infection. Scand J Infect Dis 36, 269–273.[CrossRef]
    [Google Scholar]
  • Scientific Committee on Animal Health and Animal Welfare ( 2002; ). Avian Chlamydiosis as a Zoonotic Disease and Risk Reduction Strategies. Report no. SANCO/AH/R26/2002. Brussels: European Commission, Health and Consumer Protection Directorate-General. http://europa.eu.int/comm/food/fs/sc/scah/out73_en.pdf
  • Smith, K. A., Bradley, K. K., Stobierski, M. G. & Tengelsen, L. A. ( 2005; ). Compendium of measures to control Chlamydophila psittaci (formerly Chlamydia psittaci) infection among humans (psittacosis) and pet birds, 2005. J Am Vet Med Assoc 226, 532–539.[CrossRef]
    [Google Scholar]
  • Vanrompay, D., Butaye, P., Sayada, C., Ducatelle, R. & Haesebrouck, F. ( 1997; ). Characterization of avian Chlamydia psittaci strains using omp1 restriction mapping and serovar-specific monoclonal antibodies. Res Microbiol 148, 327–333.[CrossRef]
    [Google Scholar]
  • Verkooyen, R. P., Van Lent, N. A., Mousavi Joulandan, S. A., Snijder, R. J., van den Bosch, J. M., Van Helden, H. P. & Verbrugh, H. A. ( 1997; ). Diagnosis of Chlamydia pneumoniae infection in patients with chronic obstructive pulmonary disease by micro-immunofluorescence and ELISA. J Med Microbiol 46, 959–964.[CrossRef]
    [Google Scholar]
  • Verkooyen, R. P., Willemse, D., Hiep-van Casteren, S. C., Joulandan, S. A., Snijder, R. J., van den Bosch, J. M., Van Helden, H. P., Peeters, M. F. & Verbrugh, H. A. ( 1998; ). Evaluation of PCR, culture, and serology for diagnosis of Chlamydia pneumoniae respiratory infections. J Clin Microbiol 36, 2301–2307.
     [Google Scholar]
  • Wong, K. H., Skelton, S. K. & Daugharty, H. ( 1994; ). Utility of complement fixation and microimmunofluorescence assays for detecting serologic responses in patients with clinically diagnosed psittacosis. J Clin Microbiol 32, 2417–2421.
     [Google Scholar]
  • Yung, A. P. & Grayson, M. L. ( 1988; ). Psittacosis – a review of 135 cases. Med J Aust 148, 228–233.
     [Google Scholar]
http://sgm.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46692-0
Loading

Supplementary Data

 Data loading....

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46692-0
2006-11-01
2019-02-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/11/1571.html?itemId=/content/journal/jmm/10.1099/jmm.0.46692-0&mimeType=html&fmt=ahah

Author and Article Information

 Figure data loading....

Footnotes:

Validated antibodies in this article

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error