1887

Abstract

Epidemiological studies in both humans and animals conducted in Mexico have shown that the isolation frequency of O157 : H7 is low. In a previous study, IgG antibodies against . O157, O7 and O116 LPS were found in serum samples from children and adults with no previous history of . O157 : H7 infection. The present study was designed to determine whether a similar immune response against . O157 : H7 and other antigenically related bacteria was present in bovine serum samples. A total of 310 serum samples from different herds in Mexico was analysed by microagglutination assays against different enterobacterial antigens, including . O157. Microagglutination assays were positive against . O7 (55 %), O116 (76 %) and O157 (36 %), (15 %), serotype Urbana (14 %) and subsp. (40 %). These results were confirmed using a specific ELISA with purified LPS. A positive reaction was observed against the LPS of . O7 (29 %), O116 (12 %) and O157 (22 %), . (4 %), Urbana (13 %) and subsp. (12 %). Serum absorption studies of positive serum samples indicated the existence of at least three common epitopes shared by the LPS of . O7, O116 and O157, and two others between . O157 and Urbana and . subsp. . A bactericidal assay against . O157 : H7 using 31 bovine serum samples was performed, and 22 (71 %) of these serum samples gave positive results. The data demonstrated that bovine serum showed a response against different enterobacteria, including O157, and that this response could be due to the presence of shared epitopes in the LPS of these organisms.

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2007-11-01
2024-03-28
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