Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children
Dias, Cícero A. and Teixeira, Lúcia Martins and Carvalho, Maria da Glória and Beall, Bernard,, 56, 1185-1188 (2007),
doi = https://doi.org/10.1099/jmm.0.47347-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6 %) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.,
language=,
type=