Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis
Yamazaki-Matsune, Wataru and Taguchi, Masumi and Seto, Kazuko and Kawahara, Ryuji and Kawatsu, Kentaro and Kumeda, Yuko and Kitazato, Miyoshi and Nukina, Masafumi and Misawa, Naoaki and Tsukamoto, Teizo,, 56, 1467-1473 (2007),
doi = https://doi.org/10.1099/jmm.0.47363-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.,
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