Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay
Cherkaoui, Abdessalam and Ceroni, Dimitri and Emonet, Stéphane and Lefevre, Yan and Schrenzel, Jacques,, 58, 65-68 (2009),
doi = https://doi.org/10.1099/jmm.0.47707-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.,
language=,
type=