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Volume 153, Issue 11

Responses to arsenate stress by sp. strain CNB-1 at genetic and proteomic levels Free

Abstract

sp. strain CNB-1, a chloronitrobenzene-degrading bacterium, was demonstrated to possess higher arsenate tolerance as compared with the mutant strain CNB-2. pCNB1, a plasmid harboured by CNB-1 but not CNB-2, contained the genetic cluster , which putatively encodes arsenate-resistance regulator, family II arsenate reductase, arsenite efflux pump and family I arsenate reductase, respectively, in strain CNB-1. The -negative could gain arsenate resistance by transformation with or , indicating that these two genes might express functional forms of arsenate reductases. Intriguingly, when CNB-1 cells were exposed to arsenate, the transcription of and was measurable by RT-PCR, but only ArsP was detectable at protein level. To explore the proteins responding to arsenate stress, CNB-1 cells were cultured with and without arsenate and differential proteomics was carried out by two-dimensional PAGE (2-DE) and MALDI-TOF MS. A total of 31 differential 2-DE spots were defined upon image analysis and 23 proteins were identified to be responsive specifically to arsenate. Of these spots, 18 were unique proteins. These proteins were identified to be phosphate transporters, heat-shock proteins involved in protein refolding, and enzymes participating in carbon and energy metabolism.

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  • Accepted:
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  • Published Online:
Keyword(s): 2-DE, two-dimensional PAGE , ARS, arsenate-resistance system , PMF, peptide mass fingerprint , RND, resistance-nodulation-cell division and TFA, trifluoroacetic acid.
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2007-11-01
2024-04-25
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Responses to arsenate stress by Comamonas sp. strain CNB-1 at genetic and proteomic levels
Microbiology 153, 3713 (2007); https://doi.org/10.1099/mic.0.2007/011403-0
/content/journal/micro/10.1099/mic.0.2007/011403-0
/content/journal/micro/10.1099/mic.0.2007/011403-0
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